Protocol #01
DNA isolation
Using ZymoBIOMICS™ 96 MagBead DNA Kit for isolating DNA from swabs
Prep:
 01 Vortex the collection tube with the swab for several seconds, follow by quick spin.
 02 Transfer 250 µL of the supernatant from the collection tube to the ZR BashingBead™
Lysis Tubes.
 03 Add 750 µL of ZymoBIOMICS™ Lysis Solution the Lysis Tubes. Vortex at maximum
speed for 10 minutes at the RT.
 04 Centrifuge the tubes at 10 000 g for 1 minute.
 05 Transfer 200 µL of the supernatant into a new 1.5 mL tube and add 200 µL of
ZymoBIOMICS™ MagBinding Buffer.
 06 Dispense 25 μl of ZymoBIOMICS™ MagBinding Beads to each well
 07 Transfer the tubes to a magnetic stand until the beads pellet, then aspirate and discard
the supernatant and remove the tube from the stand.
 08 Dispense 500 μl of ZymoBIOMICS™ MagBinding Buffer and mix well by pipette.
 09 Transfer the tubes to a magnetic stand until the beads pellet, then aspirate and discard
the supernatant and remove the tube from the stand.
 10 Dispense 500 μl of ZymoBIOMICS™ MagWash 1 and mix well by pipette.
 11 Transfer the tubes to a magnetic stand until the beads pellet, then aspirate, discard
the supernatant, and remove the tube from the stand.
 12 Dispense 900 μl of ZymoBIOMICS™ MagWash 2 and mix well by pipette.
 13 Transfer the tubes to a magnetic stand until the beads pellet, then aspirate, discard
the supernatant, and remove the tube from the stand.
 14 Repeat the wash (Steps 12-13)
 15 Dispense 50 μl of ZymoBIOMICS™ DNase/RNase Free Water and mix well by pipette.
 16 Transfer the tubes to a magnetic stand until the beads pellet.
 17 Transfer the supernatant (containing the eluted DNA) to a clean tube.