C8545 Developmental Biology
Lesson 3
Early Development of Amphibians and Amniotes
Jan Hejátko
Functional Genomics and Proteomics of Plants
CEITEC
and
National Centre for the Biomolecular Research,
Faculty of Science
Masaryk University, Brno
hejatko@sci.muni.cz, www.ceitec.eu
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Outline of Lesson 3
Early Development of Amphibians and Amniotes
 Oogenesis in amphibians
 Blastula formation and dorsoventral axis fomation in amphibians
 cleavage of Xenopus zygote (video)
 Gastrulation
 gastrulation of amphibians (video)
 Neurulation
 neurulation in Xenopus (video)
 Oogenesis in amniotes - chicken
 Gastrulation in amniotes – chicken
 early and late gastrulation in chicken (video)
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Outline of Lesson 3
Early Development of Amphibians and Amniotes
 Gastrulation in amniotes – chicken
 early and late gastrulation in chicken (video)
 Formation of extraembryonic tissues in amniotes – chicken
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Outline of Lesson 3
Early Development of Amphibians and Amniotes
 Oogenesis in amphibians
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Oogonia, the germ cells in the ovary of amphibians, undergo mitosis, allowing to
increase the cell number around 10.000 times.
In humans, the oogonia form limited population that does not undergo mitosis, while
in frogs, the oogonia divide, allowing thus formation of many thousands of eggs
during annual breeding season. Thus, the frogs oogonia are real stem cells that is
not the case in humans
Primary oogonium undergoes 4 additional rounds of mitosis, leading to formation of
nest of 16 cells, connected via cytoplasmic bridges.
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Huynh and Johnston., Curr Biol (2004)
Kartáčové
chromozómy
Oogenesis in Xenopus vs. oogenesis in Drosophila
Zárodečný
váček
Vitellogenin in the bloodstream
Animal pole
Vegetal pole
These cells start to grow, enter meiosis and are called primary oocytes. In contrast to
Drosophila, where only one of the oogonium progeny (oocyte) will undergo meiosis (see the
inset on the right-hand side and the Lesson 02), here all 16 cells will develop into complete
egg. The nucleus of oocytes is called germinal vesicle (similarly to Drosophila).
During extended prophase I (about 2 weeks) the recombination occurs.
Chromosomes get uncoiled and will develop into brush-like form - they are called the
lampbrush chromosomes. These uncoiled chromosome regions contain the
transcriptionally active DNA that allows high level of RNA production needed for oocyte
growth (see also later).
Each primary oocyte becomes surrounded by follicle cells, the chromosomes recompact
and the yolk becomes stored. Yolk is produced in the liver and yolk precursor vitellogenin is
transported by bloodstream.
In the oocyte, vitellogenin is processed to the yolk platelets.
There is apparent polarity in the oocyte. The nucleus is located in the hemisphere
containing more pigment granules, at the animal pole, while yolk accumulates at the
opposite, vegetal pole.
There is no anterioposterior axis formed yet, however, there is apparent animal-vegetal
pole asymmetry. The vegetal pole corresponds to the position of previously formed
cytoplasmic bridge.
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Huynh and Johnston., Curr Biol (2004)
Sperm entry in the animal hemisphere
determines the future dorsoanterior position
The mechanisms of the oocyte polarization and early axis formatioon are conserved
in Drosophila and vertebrates as discussed in the previous lesson. The figure above
shows similarities between the early steps of Drosophila and Xenopus oogenesis.
In both Drosophila and Xenopus, the oocyte inherits an anterior–posterior axis of
symmetry from the cyst divisions.
Specific cytoplasmic components (green) accumulate in a depression above the
nucleus (red). The oocyte then polarises along this axis when the somatic follicle
cells surround it. It is suggested that a signal coming from the follicle cells may
trigger this polarisation.
This polarisation is clearly seen in Drosophila with the translocation of specific
cytoplasmic proteins, mRNAs and the centrosomes to the posterior of the oocyte
(black arrow). The situation is less clear in Xenopus, as the cell rounds up and
seems to lose any polarity. However, it is proposed that the same components that
were located above the nucleus after the cyst division, are now part of the Balbiani
body on the vegetal side (green sphere).
At the following stage, these components migrate to the posterior/vegetal cortex of
the oocyte. This has been clearly demonstrated in Xenopus, and is here
hypothesized for Drosophila . Both oocytes then enter the vitellogenic stages
(Huynh and Johnston., Curr Biol , 2004).
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Internal Transcribed Spacer (ITS)
5’ External Transcribed Sequence (ETS)
3’ ETS
Nuceloli located (approx. 1500)
Outside the nucleoli
20K vs. 400 copies in
oocytes and somatic
cells, respectively
Cytosol
Nucleus
The tremendous growth of the amphibian egg (1,3 mm in Xenopus) is ensured by the
synthesis of the proteins that are produced on multiple ribosomes (thus, not imported from
nurse cells like is the case of Drosophila).
For that purpose, the genes encoding ribosomal RNA (rRNA) are amplified specifically in
the egg (genes for 28S,18S and 5.8S rRNA are located in1500 extra nucleoli in the egg).
28S,18S and 5.8S rRNA are encoded by single 45S rRNA gene. 45S rRNA contains ITS
(for Internal Transcribed Spacer) that refers to a piece of non-functional RNA situated
between structural ribosomal RNAs (rRNA) on a common precursor transcript.
Read from 5' to 3', this polycistronic rRNA precursor transcript contains the 5' external
transcribed sequence (5' ETS), 18S rRNA, ITS1, 5.8S rRNA, ITS2, 26S rRNA and finally
the 3'ETS. During rRNA maturation, ETS and ITS pieces are excised and as non-functional
maturation by-products rapidly degraded.
In case of 5s rRNA (which are not located in nucleoli), specific genes for 5sRNA are
activated in the egg (there is a specific sequence of those egg-specific 5s RNAs, which are
of 19,600 copies in comparison to approx. 400 copies of somatic cell 5s rRNA genes.
The number of nucleoli increases. In nucleoli, the ribozomal RNA genes are located,
transcribed, new ribozomes are assembled and transported to the cytosol.
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Hormonální stimulace
hormony hypofýzy
(gonádotropin)
Zárodečný váček
Intracellular perceived progesterone
signal induces oocyte maturation.
Progesteron
Folicle
disassembles,
release to oviduct
• sperm cell nucleus
• centrosome
Sperm
cell
entry
The fully grown oocyte resides in the ovary. External conditions affect production of pituitary
gonadotropins, which induce production of progesterone by the follicle cells.
Progesterone signal is sensed by the specific receptors in the oocyte and induces oocyte
maturation.
Germinal vesicle breaks down, the nucleolema disintegrates and meiosis I proceeds.
Follicle disassembles and mature oocyte is released to the oviduct, transported to cloaca
and spawned. In Xenopus, the fertlization takes place in the prophase of meiosis II.
Spermatogenesis takes place in the seminiferous tubules of the male. Spermatogonia act
as stem cells, mitotic descendants differentiate and enter meiosis, forming thus four haploid
spermatids.
Most of the information at the time of fertlization resides in the cytoplasm of the egg.
Besides its DNA, sperm cell contributes centrosome.
Acquiring of centrosome is sufficient to activate further development events in the haploid
egg. However, amphibian eggs are easy to activate even via pricking with the pin.
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Outline of Lesson 3
Early Development of Amphibians and Amniotes
 Oogenesis in amphibians
 Blastula formation and dorsoventral axis formation in amphibians
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• Completion of meiosis II
• Syngamy
Just before fertlization, sperm accumulates preferentially at the animal cortex.
However, the sperm might enter the egg anywhere in the animal hemisphere.
After the sperm cell and centrozome gets inside the egg cell, male centrozome
becomes an organizing centre of female tubulin that will form a growing sperm
aster.
Oocyte completes meiosis II and syngamy occurs.
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Prospective ventral pole
Prospective dorsal pole
Male centrosome
Zóna
střihu Šedý
srpek
At the moment of fertilization, there is still no asymmetry, no dorsoventral or
anteroposterior axis as is the case of egg in Drospohila, but only animal and vegetal
poles could be distinguished.
However, the fertilization breaks this radial symmetry and via the sperm aster
formation it induces acquisition of the bilateral symmetry. Fertilization also starts the
aligning of the microtubules in a shear zone in a direction necessary for the cortex
cytoplasm rotation. The sperm entry point defines the plane of rotation (the
meridian) that goes through the sperm entry point and the animal and vegetal poles.
In the case of parthenogenesis, the movement starts in random meridian to induce
the bilateral symmetry.
For the future formation of dorsoventral axis, the movement of the egg cortex with
regard to the core is critical. The movement of the cortex (about 4 um thick layer at
the egg periphery) starts at about 0,4t-0,8t, where t is the time between fertilization
and first cleavage of the egg.
The gray crescent zone gets cross dissected by the first cleavage (see the video)
and it will became the place of high gastrulation activity (see later).
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Motor molecules
located along the microtubules
The movement is facilitated by the arrays of microtubules that are oriented in
parallel with the egg surface and located at the interface between cortex and the
core.
There are motor molecules associated with the microtubules that are responsible for
the movement. The movement is oriented towards the positive pole of
microotubules, which point towards the future dorsal pole of the embryo.
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Zbytnělá cementová žláza
(adhezivní orgán)
If the rotation is disturbed (via e.g. UV light, which prevents microtubule
polymerization), there is no dorsal structures development (the embryo will contain
a gut and blood cells, but no muscles or nervous system.
Vice versa, if the movement is stimulated by heavy water, hyperdorsalized embryos
are formed.
Finally, modification of the movement via e.g. centrifugal forces, extra cortical
rotation could be imposed, leading to Siamese twins formation.
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16-64 cells: morula
Complete cell divisions
(including cytokinesis)
First 12 rounds of cell
divisions at high peace
(every 15 min. each)
Morus nigra (mulberry)
212 (approx. 4000) cells
128 cells: blastula
Early after fertilization, the cleavage of the egg starts.
In the stage between 16-64 cells the embryo resembles the fruit of mulberry,
therefore it’s being called morula (from Latin “morus”).
In the stage of 128 cells blastocoel appears and the embryo is called blastula.
During this stage, egg becomes a “DNA factory” that produces DNA, proteins and
plasma membrane components at a very high speed.
In comparison to Drosophila, where syncitium is formed followed by cellularization,
in amphibians the complete cell division (i.e. including cytokinesis) takes place. DNA
polymerases, histone mRNA and membrane precursors are stockpiled in the egg
during oogenesis.
First 12 rounds of cell divisions occurs at a high peace, every 15 minutes each,
leading to formation of 212 cells (approx. 4000 cells).
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Dělící rýha
Important molecular events precede further development at the midblastula
transition (MBT)
• Induction of transcription
• Acquiring potential of cell motility
• Slowing down the cell division cycle
Newly formed cells are divided by cleavage furrows that introduce part of the “old”
plasma membrane into the cell-to-cell interfaces. At the position of the old
membrane, the cells are connected with what is called “tight junctions” (for the
underlying molecular mechanisms see in later chapters).
At the stage of midblastula transition (MBT), substantial molecular changes occur
that precede the following developmental events (i.e. developmental potential
specification of different blastula portions).
First, there is an induction of transcription, second, some cells acquire potential
of motility and third, the cell division cycle slows down (G1 and G2 phases
become a part of the cell cycle).
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Outline of Lesson 3
Early Development of Amphibians and Amniotes
 Oogenesis in amphibians
 Blastula formation and dorsoventral axis fomation in amphibians
 cleavage of Xenopus zygote (video)
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Budoucí
předledvina
Somite
Notochord
Leading edge mesoderm
Vedoucí okraj mezodermu
Fate map of midblastula, as
defined by transplantation
experiments
The invagination during
gastrulation starts here
At the stage of midblastula, the fate map (i.e. the developmental fate of differently
stained blastula cells) correspond to the egg, as there are no cell movements yet.
Originally defined fate map of Xenopus (above) has recently been updated, with
slight modifications of the prospective ventral and dorsal poles (below).
The specification map (or also competence map) is obtained by explantation
experiments, i.e. pieces of the blastula are transferred to other embryos or to the
tissue culture medium and allowed to develop into pre-specified tissue. However,
the competence of cells to form specific tissue after excision from embryo might
differ in comparison to the intact embryo (see next slide).
Leading edge mezoderm (LEM) starts the invagination during gastrulation (see
later).
Experiments by Connie Lane and Bill Smith suggest that the prospect ventral and
dorsal poles are located closer to the vegetal and animal poles, respectively (shown
by arrows pointing to these new positions designated as V’ and D’).
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When the cells from the animal pole (animal cap cells) are removed, they are able
to from ciliated epidermis in the tissue culture medium.
The cells originating from the vegetal pole form gutlike endoderm cells.
When the cells are combined, however, they will produce mesodermal structures.
These findings let to a hypothesis that vegetal pole cells are source of graded
inducing factor, which induces mesoderm formation. Recent findings suggest that
this is more complex with generalized induction of mesoderm and anterior
endoderm followed by secondary induced signal that allows dorsal mesoderm
formation.
This concept of induction signals, however, does not exclude predetermination
based on the egg deposited signaling molecules. This seems to be the case, at
least partially.
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Outline of Lesson 3
Early Development of Amphibians and Amniotes
 Oogenesis in amphibians
 Blastula formation and dorsoventral axis fomation in amphibians
 cleavage of Xenopus zygote (video)
 Gastrulation in amphibians
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Blastopor
(prvoústa)
Budoucí
nervová trubice
Dorzální
IMZ(Spémanův
organizátor)
Involuting marginal zone
Involující marginální zóna (IMZ)
Non-involuting
marginal zone
Neinvolující
marginální zóna
(NIMZ) Limit of
involution
Hranice
involuce
Gastrulation starts in the position that is approximately opposite to the sperm entry
and where the vegetal cortex got over the original animal pole (see the red arrow in
the inset).
Then, the so called bottle cells start invaginate, leading to the blastopore formation
that is delimited by the blastopore lip.
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Entry of sperm
cell
Dorzální okraj
(prvoúst)
The bottle cells at the dorsal lip become narrow and attenuated, which allows their
ingression deeper into the other cell layers. Thanks to the pressure of surrounding
cells the bottle cells appear to pull on their thin cell “necks”.
The anatomical structure arising from this invagination is known as blastopore. The
beginning dimple is called the dorsal lip of the blastopore and it occurs in the
position where due to the rotation of the cortex, the interface between original
vegetal and animal portions of the egg get opposed to each other (see the red and
blue arrows, respectively).
Once involuted, the deep marginal zone cells actively migrate, moving the
archenteron anteriorly.
The invaginating cells of the dorsal and ventral lip develop into mesoderm,
surface cells of the majority of the former animal pole develop into epidermal
and neural ectoderm and the cells developing from the majority of the former
vegetal pole will develop into endoderm.
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Oblast epibolie (radiální interkalace)
Dorzální okraj prvoúst
Ventrální
okraj prvoúst
Žloutková
zátka
deep marginal cells move anteriorly
The invagination of the vegetal pole cells leads to the tensions that result into
extension of the portion of the embryo surface, a process called epiboly.
The movement of deep marginal cells anteriorly leads to the collapse of the
blastocoel, while new interior cavity called archenteron is formed.
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Complex movement of the cells precedes the invagination, leading to abortion of the
blastocoel and new interior cavity formation, the archenteron.
Dorsal portion of the embryo undergoes epiboly and neurulation is finished (for the
more detailed description of neurulation see later text).
At the end of gastrulation in amphibians, similarly to Drosophila there is an internal
cavity, lumen of the future gut surrounded by endoderm. In contrast to Drosophila,
the internal cavity is blind ended, with the future mouth being developed on the
closed pole.
The surface is created by epidermal and neural ectoderm and between them there
is remaining intermediate mesodermal layer.
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Outline of Lesson 3
Early Development of Amphibians and Amniotes
 Oogenesis in amphibians
 Blastula formation and dorsoventral axis fomation in amphibians
 cleavage of Xenopus zygote (video)
 Gastrulation in amphibians
 gastrulation of amphibians (video)
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Radiální interkalace Mediolaterální interkalace
The process of epiboly is connected with the surface extension. This is allowed by
cell layers intercalation, leading to the reduction of the cell layer thickness, but
increasing its surface. There is radial and mesodermal intercalation.
The radial intercalation leads to the intercalation of surface cells into the deeper
cell layers, while the mediolateral intercalation allows convergence of the cells
from the same layer leading thus to both narrowing of the cell layers in the
mediolateral direction and their extension
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Dorsal IMZ
(Spemann organizer)
Dorzální IMZ
(Spémanův organizátor)
Cells adjacent to the dorsal lip of the blastopore act as a so called Speman
organizer.
When the cells of the Speman organizer are transplanted to other embryo, they are
able to induce secondary invagination, leading to the second axis formation with
secondary neural tube and adjacent muscles and some other structures, finally
resulting into conjoined twin embryo.
As shown in experiments with distinctly pigmented embryos, most of the secondary
axis tissue originates from the host. Thus, the Speman organizer is able to induce
change of the developmental fate in the neighbouring tissue.
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Outline of Lesson 3
Early Development of Amphibians and Amniotes
 Oogenesis in amphibians
 Blastula formation and dorsoventral axis fomation in amphibians
 cleavage of Xenopus zygote (video)
 Gastrulation
 gastrulation of amphibians (video)
 Neurulation
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Neurální brázdička
(rýha)
Tvořící se
neurální val
Neurální ploténka
Dorsal mesoderm acts as an inductor of the dorsal ectoderm formation that turns
into neural tube, the process called neurulation. The molecular mechanism of this
process will be discussed later.
The cells of the dorsal ectoderm are induced by the notochordal mesoderm to
change their shape from square to rectangular, forming what is called neural plate.
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Change of the cell shape due to microfilament action
Due to microfilament action, the neural plate cells change their shape that is one of
the processes necessary for the rolling of the plate into neural tube. The two outer
edges of the plate approach one other in the dorsal midline.
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Buňky neurální lišty
Fůzované
neurální valy
Místa ohybu
Finally the two “lips” of the plate meet and fuse.
In some other vertebrates, e.g. in fishes, the neural tube is formed in a different
way-the chord cells form a rod, which then becomes whole in the center.
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Outline of Lesson 3
Early Development of Amphibians and Amniotes
 Oogenesis in amphibians
 Blastula formation and dorsoventral axis fomation in amphibians
 cleavage of Xenopus zygote (video)
 Gastrulation
 gastrulation of amphibians (video)
 Neurulation
 neurulation in Xenopus (video)
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Mezentérium
(v dospělosti
peritoneum)
Ledviny
Aorta
Somit
Struna hřbetní
(chorda)
Neurální trubice
Genitální lišta
Epidermis
Prvostřevo
Coelom
Somatopleura
Somatic mesoderm
Splanchnopleura
Splanchnic mesoderm
Mesoderm and endoderm will form many organs. The pattern of transforming an
epithelial sheet into a tube or sphere is repeated in the formation of many organs of
the body.
The endoderm of the archenteron (epithelium) will bulge out (evaginate) in various
places along its length into the adjacent mesoderm and will form salivary glands,
lung, liver, pancreas and thyroid.
The dorsal most mesoderm subjacent to the midline of the neural plate undergoes
intercalation, stack of cells swell and form what is called notochord. Notochord
structure is typical for vertebrates, it is significant skeletal structure in lower
vertebrates but becomes calcified and buried within the bony spinal column of
terrestrial vertebrates.
On each side of the notochord, mesoderm will develop into bilaterally symmetric
blocks called somites. Many other structures derive from somites, e.g. most of the
skeletal muscle, all dermis and cartilage surrounding the spinal chord.
More ventrolateral mesoderm will splits into two layers, the more peripheral that
associates with the outer ectoderm forms somatic mesoderm layer, the other
mesoderm layer covers endoderm of the developing gut, forming thus splanchnic
mesoderm. The space between these two layers is called coelom.
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Outline of Lesson 3
Early Development of Amphibians and Amniotes
 Oogenesis in amphibians
 Blastula formation and dorsoventral axis fomation in amphibians
 cleavage of Xenopus zygote (video)
 Gastrulation in amphibians
 gastrulation of amphibians (video)
 Neurulation
 neurulation in Xenopus (video)
 Oogenesis in amniotes - chicken
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Infundibulum
(nálevka)
Magnum
Isthmus (úžina)
Děloha
Vejcovod
Vaječník
Zárodečný terčík
Oogenesis occurs in the specialized female reproductive tract. Ovary produces
oocytes that undergoes meiosis and accumulates yolk that is produced in the liver.
The cytoplasm is located aside as a small coherent disc.
Ones the oocyte is released from the ovary, it is fertlized and starts travel through
the oviduct that contains specialized departments.
In the magnum, white is synthesized and blastodisc, another term of blastoderm
that undergoes meroblastic or partial cleavage, is formed. White consists mainly of
ovalbumin and lysozym that has antibacterial effects.
In the isthmus, the complex membrane constitutes around the white and in the
uterus, and elaborate calcium carbonate shell is deposited around the egg.
Cells of the upper portion of blastodisc, called epiblast are divided from the
remaining yolk by subgerminal cavity. During passing through the oviduct, some
cells of the epiblast shed and are falling down to the cavity, where they die. That
leads to the thinning of the central area, area pelucida.
The cells at the periphery of the blastodisc are syncitial and are called marginal
zone (see also following slide).
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Outline of Lesson 3
Early Development of Amphibians and Amniotes
 Oogenesis in amphibians
 Blastula formation and dorsoventral axis fomation in amphibians
 cleavage of Xenopus zygote (video)
 Gastrulation
 gastrulation of amphibians (video)
 Neurulation
 neurulation in Xenopus (video)
 Oogenesis in amniotes – chicken
 Blastula formation in amniotes - chicken
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Migrující buňky
hypoblastu
Rýhovací
dutina
Žloutek
Posteriorní
marginální zóna
Area
opaca
Area
pellucida
Primární hypoblast
Sekundární hypoblast
Egg laying:
60,000 cells,
about 1 mm in
diameter
At the time of the egg laying, the blastodisc consists of about 60,000 cells and is
about 1 mm in diameter. The central part of the blastodisc is more transparent,
because its constituent cells have less yolk and it is called area pelucida. There are
more yolky cells at the periphery, called area opaca.
Though mostly radial symmetric, the developmental bias is already predetermined
as shown by surgical experiments (see the next slide).
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The future posterior pole could be morphologically recognized as a thickening at the
periphery of blastodisc (in the marginal zone).
Then, the blastodisc was cutted in pieces and let to develop in the culture. The
percentage of pieces that were able to form axis under these conditions was scored.
These experiment have clearly shown that individual zones of blastodisc are already
prespecificed at that developmental stage.
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Migrující buňky
hypoblastu
Rýhovací
dutina
Žloutek
Posteriorní
marginální zóna
Area
opaca
Area
pellucida
Primární hypoblast
Sekundární hypoblast
Köllerův srpek
There is a role of graviotropism in the
anteroposterior axis formation in birds
(endoblast)
During development, the area pelucida becomes two-layered structure.
At the prospective posterior end, there is located proliferative tissue called Köller’s
sickle. From this region, a new cellular layer advances, forming thus a lower tissue
layer, called hypoblast.
Cells detaching from the anterior portion of the area pelucida might be incorporated
into the developing hypoblast (sometimes called also endoblast).
Gravitropism plays a role in the specification of the anteroposterior axis. In the
oviduct, egg is always oriented with the posterior pole up (see the inset).
When the eggs were removed during that process and reoriented, the prospective
posterior pole always appears at the upper side of the egg. The underlying
molecular mechanism is still not clear. Possibly, the shading of cells from the
epiblast might be somehow connected, as it always occurs at the prospective
posterior end.
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Blastoderm
(blastodisc)
Zárodečný terčík
Does hypoblast determine the
anteroposterior axis of epiblast?
Köllerův
srpek
What is the type of inductive interaction between lower layers of the blastodisc and
epiblast in the determination of the further development is not completely clear.
Recently, the original idea that hypoblast induces anteroposterior axis in the epiblast
was challenged by the series of surgical experiments.
When the hypoblast region of different size was rotated towards developing epiblast
and cultured, no changes were apparent in the primitive streak formation. This
suggests that anteroposterior axis formation in epiblast is already predetermined at
that developmental stage (see the slide).
However, other experiments support some level of communication between
hypoblast and epiblast. When the hypoblast was removed together with marginal
zone, axis formation failed. On the other hand, leaving either hypoblast or the
marginal zone present allows axis formation to occur.
Thus, very probably, hypoblast and marginal zone are a source of the inductive
signal, similarly to the situation of the mesoderm formation in amphibians. The
molecular determinants are being recently identified and will be discussed in later
lessons.
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Outline of Lesson 3
Early Development of Amphibians and Amniotes
 Gastrulation in amniotes – chicken
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Hensenův uzel
Area opaca
Primitvní
proužek
Area pellucida
Gastrulation in birds starts by the oriented movement of cells from the lateral
portions of the posterior epiblast towards the midline. That suggests that the
individual cells have already predetermined whether they will move or not, in other
words, they already acquired they future identity (see also the video).
The migrating cells create in the midline a thickening what is called primitive
streak. The anterior end of the streak is called Hensen’s node that plays an
important organizing role (see later).
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Primitive streak plays a similar role like the bottle cells in case of amniotes.
This is the place where gastrulation starts, and via complicated cell movements allowing to develop
all three germ layers: ectoderm (the cells that will remain on the surface of epiblast), endoderm (the
movement of these cells displaces hypoblast cells laterally) and mesoderm (the cells in between).
Importantly, notice that all embryonic tissue originate from epiblast. The epiblast cells migrating
and diving into the hypoblast displace the original hypoblast to the margins, where it develops into
extraembryonic endoderm (see below).
Cells at the margins of the the area opaca divide and blastoderm edges spread all over the enormous
yolk. That allow future formation of extraembryonnic tissues that will become a fundament for the
membranes surrounding the whole embryo-extraembryonic ectoderm (originating from marginal
zone), extraembryonic endoderm (from original hypoblast) and extraembryonic mesoderm (from
middle layer of cells).
The Hansen’s node is an organizing center of similar importance as is the Spemann organizer in
amniotes.
It is a hub of complex morphogenic activties and cell interactions. E.g. it is a structural way station for
passing cells and a centre of cell proliferation. The cells spread from here forming the notochord and
brain (what is called a “head process”).
The Hansen’s node starts moving towards the posterior end of the primitive streak and leaves behind
differentiated neural plate, notochord and the developing somites.
Hansen’s node was shown to have similar function as have the cells of the dorsal lip in amniotes
(Spemann organizer). Transplantation of the Hansen’s node leads to the ectopic formation of neural
tube and somites that are of host origin; thus, it is a primary inducer.
Hansen’s node could be surgically extirpated (removed); however, ones the tissue heal, new node
regenerates in a position where the tissues knit together. Thus, the Hansen’s node is a
selforganizing morphogenic centre, probably based on the interactions of surrounding tissues.
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Somatický
mezoderm
Splanchnický
mezoderm
Střední
mezoderm Somity
Nervová
trubice
In a cross section of the developing chicken embryo, all the germ layers and organ
anlagen (earliest beginnings of an organ, from German) are almost identical in
comparison to Xenopus embryo:
Neural tube and notochord could be distinguished at the apical portion and bilateral
somites are apparent.
More laterally, intermediate mesoderm forms that will develop into kidney.
Most laterally, the mesoderm forms thin spreading sheets that, again similarly to
amphibians, form two layers, somatic mesoderm (fusion of the laterall mesoderm
and ectoderm) and splanchnic mesoderm (fusion of the laterall mesoderm and
entoderm). The space between them is coelom.
In comparison to amphibians (see the inset), the endodermal gut is missing and
forms later.
The second major difference is the future formation of extraembryonic membranes
that will enclose the embryo and that demonstrate an adaptation of terrestrial
vertebrates.
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Outline of Lesson 3
Early Development of Amphibians and Amniotes
 Gastrulation in amniotes – chicken
 early and late gastrulation in chicken (video)
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Outline of Lesson 3
Early Development of Amphibians and Amniotes
 Gastrulation in amniotes – chicken
 early and late gastrulation in chicken (video)
 Formation of extraembryonic tissues in amniotes - chicken
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Extraembryonální coelom
Somatopleura
Splanchnopleura
Endoderm
Žloutek
Origin of extraembryonic tissue
Via complicated invaginations and movements, four membrane sacs are formed of
the extraembryonic tissue. The formation of the extraembryonic sacs represent
adaptation for the terrestrial environment.
First, the double layered surroundings of the embryo are formed from the ectodermsomatic
mesoderm. The ectoderm-mesoderm layer in the zona pellucida region
starts movements that undercut margins of the embryo and demarcate thus the
extraembryuonic tissue from the embryo proper.
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Hlavový ohyb amnionu
Ocasní ohyb amnionu
Extraembryonální coelom
Somatopleura
Splanchnopelura
Endoderm
Žloutek
The extraembryonic tissue layers grow up and cover the embryo from both head
and tail poles. The newly formed double ectoderm-mesoderm layer that starts to
cover the embryo is called amnion.
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Embryo
Střevo
Amniová dutina
Amnion
Alantois
Seróza (chorion)
Extraembryonální
endoderm
Future yolk sac
Budoucí
žloutkový váček
Further growth of the head and tail folds of the amnion leads to the complete
coverage of the embryo into what is called amniotic cavity and the double layers of
the amnion eventually separate.
The outer ectoderm-mesoderm layer will eventually enclose the entire mass of the
egg and become epithelial layer under the shell through which gas can exchange.
This layer is called chorion.
The extraembryonic endoderm (originating from the hypoblast in the area opaca
and area pellucida, see above) together with splanchnic mesoderm cover the yolk
and become highly vascularized. The resulting structure is called yolk sac. The
enzymes in the yolk sac allow mobilization of the enclosed yolk and via the blood
stream the nutrients are supplied to the embryo.
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Amniová
dutina
Chorioamniová
dutina
Vychlýpením zadního střeva
vzniká budoucí alantois
(splanchnic)
uric acid accumulation
The evagination of the hindgut that is composed of endoderm and splanchnic
mesoderm into the space between yolk sac and chorion leads to the formation of
another sac that is called allantois.
Allantois contains products of the embryo metabolism, the crystals of the uric acid.
As the uric acid acummulates, the allantois expands but this is compensated by the
reduction of the yolk sac.
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Chorioalantoidní
membrána
Alantois
Seróza (chorion)
In birds, the allantois becomes very large, fills the space between the chorion and
yolk sac and fuses with chorion into singe chorioallantoic membrane. The mebrane
is highly vascularized and allows gas exchange through the shell.
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Key Concepts
 First source of asymmetry originates from the oogenesis both in
Drosophila and Xenopus.
 In Xenopus, another important source of asymmetry leading to
breaking of the virtual radial symmetry of the egg and dorsoventral
axis specification is the sperm entry that induces cytoplasm
rotation.
 These processes result into Speman organizer differentiation and
allow specification of the cell fate during blastula formation.
 Gastrulation allows further delimitation of the developmental fate.
 Amniotes developed terrestrial adaptations that are of
extraembryonic origin.
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Discussion