Final task - RNA analysis
Analyze RNA-seq samples prepared with QuantSeq 3’ mRNA-Seq Library Prep Kit
from human cell-line. There are 3 replicates of wildtype samples and 3 replicates of
VeR treated samples. Raw reads in fastq format are provided for all 6 sequenced
samples. Ligated UMIs were removed from the reads and written to the reads headers.
The goal is to identify genes with statistically significant expression changes.
The expected deliverables are:
1. Table with a list of differential expressed genes (can be a resulting DEseq
table)
2. MultiQC report in html format summarizing pre- and post-alignment QC
3. A workflow that can reproduce these results with a single command