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Structural Virology
Lecture 10
Pavel Plevka
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Virus vaccines
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Antibody dependent enhancement
dengue
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Antibody dependent enhancement -
coronaviruses
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Smallpox
• killed an estimated 400,000
Europeans annually during
the 18th century.
• was responsible for a third of
all blindness.
• of those infected, 20–60%—
and over 80% of infected
children—died
• responsible for an estimated
300–500 million deaths
during the 20th century
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Vaccine Types
• Attenuated viruses
• Inactivated viruses
• Virion subunits
• Recombinant viruses
• Virus-like particles
• Synthetic peptides
• DNA vaccines
© 2012 John Wiley & Sons Ltd.
www.wiley.com/college/carter
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© 2012 John Wiley & Sons Ltd.
www.wiley.com/college/carter
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© 2012 John Wiley & Sons Ltd.
www.wiley.com/college/carter
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© 2012 John Wiley & Sons Ltd.
www.wiley.com/college/carter
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www.wiley.com/college/carter
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Learning outcomes
• explain how virus diseases are controlled with
vaccines
• evaluate the types of virus vaccine in medical
and veterinary use
• evaluate types of virus vaccine that are experi-
mental
• describe methods used to manufacture virus
vaccines
• evaluate procedures designed to ensure the
safety of virus vaccines
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Antiviral compounds
© 2012 John Wiley & Sons Ltd.
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HIV HCV
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Picornavirus virion
Plevka et al. Science 2012.
1. Genome delivery into cytoplasm
Rossmann Prot Sci, 1994
Pocket factor in EV71
Smith TJ, et al. Science, 1986
Inhibition of EV71 by WIN 51711
Plevka et al. PNAS 2013
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Learning outcomes
• evaluate procedures used to develop new antiviral
drugs
• describe the modes of action of selected antiviral
drugs
• discuss virus resistance to drugs
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Prions
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Brain with Scrapie
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Normal and misfolded form of prion
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Creutzfeldt-Jacob disease x BSE
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Viroids
© 2012 John Wiley & Sons Ltd.
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Virophage - Sputnik