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Structural Virology
Lecture 6
Pavel Plevka
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Parvoviruses
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Parvoviruses
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Minute virus of mice genome
organization
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Kozak’s sequence:
gccRccAUGG
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• give examples of parvoviruses and explain
their importance;
• describe the parvovirus virion;
• outline the main features of the parvovirus
• genome;
• describe the replication cycle of parvoviruses;
• explain the difference between autonomous
and defective parvoviruses.
Learning outcomes
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Reoviridae
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NSP4 induces:
• calcium ion-dependent chloride secretion, disrupts SGLT1 transporter-mediated
reabsorption of water
• reduces activity of brush-border membrane and possibly limits activity of enteric
nervous system
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Reoviridae
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• describe the rotavirus virion;
• discuss the main events of the rotavirus
replication cycle;
• explain how rotaviruses cause disease.
Learning outcomes
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Picornaviruses
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FMDV
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Rhinoviruses
– 40% of common cold cases
– economic losses $16bn/year in USA & EU
McMinn et al. Clin Infect Dis 2001. Image by Heng Soy, KI Media 2012.
Human picornaviruses
Enteroviruses (EV71)
– hand-foot-and-mouth-
disease
– encephalitis
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Picornavirus genome organization
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Picornavirus virion
Plevka et al. Science 2012.
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Rossmann Prot Sci, 1994
Pocket factor in EV71
Smith TJ, et al. Science, 1986
Inhibition of EV71 by WIN 51711
Plevka et al. PNAS 2013
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Virion assembly
Knipe et al. Fields Virology, 2007
4. Virion assembly mechanism
Genome packaging into
pre-formed capsids
Capsid assembly around
condensed genome
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• give examples of picornaviruses and explain their
importance;
• describe the picornavirus virion;
• describe the picornavirus replication cycle;
• discuss picornavirus recombination;
• describe experimental systems used for picornavirus
studies.
Learning outcomes