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Structural Virology
Lecture 7
Pavel Plevka
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Rhabdoviridae
Virions are bullet shaped 180 nm long and 75 nm wide.
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Vesicular stomatitis virus
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Rabies virus
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Attachment and cell entry
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Virion organization
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Genome organization and sequential transcription
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Sequential transcription
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Negative-stranded RNA virus polymerase stuttering
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Negative-stranded RNA virus replication
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Translation and post-translational modifications
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Budding
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Viruses with minus or ambisense RNA genomes
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Arenaviridae (Lassa fever)
Fever, facial swelling, muscle fatigue,
conjunctivitis and mucosal bleeding.
Gastrointestinal tract
• Nausea
• Vomiting (bloody)
• Diarrhea (bloody)
• Stomach ache
• Constipation
• Dysphagia (difficulty swallowing)
• Hepatitis
Cardiovascular system
• Pericarditis
• Hypertension
• Hypotension
• Tachycardia
Respiratory tract
• Cough
• Chest pain
• Dyspnoea
• Pharyngitis
• Pleuritis
Nervous system
• Encephalitis
• Meningitis
• Hearing deficit
• Seizures
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Ebola virus
Symptoms
• Fever
• Severe headache
• Muscle pain
• Weakness
• Fatigue
• Diarrhea
• Vomiting
• Abdominal (stomach)
pain
• Unexplained hemorrhage
(bleeding or bruising)
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Ambisense virus translation
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Learning outcomes
• discuss the importance of rabies virus, vesicular stomatitis
virus and other minus-strand RNA viruses
• describe the rhabdovirus virion
• outline the main characteristics of the rhabdovirus
genome
• discuss the replication cycle of rhabdoviruses
• explain the term ‘ambisense genome’
• discuss the development of reverse genetics procedures
for minus-strand RNA viruses
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Orthomyxoviridae - Influenza
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Hemagglutinin
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Membrane fusion
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Cap snatching
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Alternative splicing
Leaky scanning
Ribosomal frameshift
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Antigenic drift x antigenic shift
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Learning outcomes
• Describe structure of Influenza A virion
• Describe the Influenza A genome
• Describe Influenza A replication cycle
• Explain antigenic drift and shift
• Explain membrane fusion mechanism
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Retroviridae
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Reverse transcriptases
Murine leukemia virus (MLV)
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Reverse transcriptase
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Learning outcomes
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