Historical milestones of nanobiotechnology Provide some examples of nano-scaling effects Carbon-based nanomaterials Graphene, preparation and applications Examples of metal-based nanoparticles Magnetic particles – preparation, applications Preparation, characterization and modification of Au nanoparticles Surface plasmon resonance for bioanalysis What are typical applications of nanoparticle bioconjugates? Surface groups for bioconjugations Principles of click-chemistry Electrophoresis of nanoparticles Which interactions are important for stability of nanomaterial dispersions? How can be estimated the molar concentration of nanoparticle dispersion? Which methods can be used for size characterization of nanoparticles and their bioconjugates? Which methods can be used for purification of nanoparticles and their bioconjugates? Light scattering and nanotracking analysis Electron microscopies Single-particle based techniques Quantum dots – principles, preparation, properties Photon up-conversion luminescent nanoparticles Electrochemical techniques useful for nanomaterials Field effect transistors and applications Principles of biofuel cells Nanopores – structure and applications Examples of point-of-care testing Describe photolithographic process Examples of nanofabrication techniques Microfluidic systems and their applications What is Lab-on-Chip and Point-of-care? Describe fundamental principles and aims. What are the approaches to cell sorting? What are the main approaches to deposition of biomolecules at nanoscale? Describe main biosensing schemes and principles of nanosensors with electrochemical detection. Principles of DNA assays, biochips Types of scanning probe microscopy techniques Microcantilevers, preparation, materials, principal parameters What is the effect of AFM tip sharpness on resolution of atomic force microscopy? Can be microscopy glass used for imaging of individual molecules, such as protein, DNA, etc.? Describe the procedure for immobilization of protein molecules on the mica surface for AFM microscopy. Describe the procedure for immobilization of DNA molecules on the mica surface for AFM microscopy. Make an easy sketch of force-distance curve and describe its basic properties. Describe basic modes of AFM microscope operation Principles of SNOM Principles of STM Piezoelectric actuators in AFM systems Nanobiotechnologies in biomedicine Delivery of drugs to tumors Materials suitable for implants