C7876en – Schedule


26.2.             9:00-12:00 (C05/107) – Tools used in Gene Technologies: primer and qPCR probe
design, design of oligos for different cloning systems, analysis of nucleotide and protein
sequences, design of site-directed mutagenesis oligos, CRISPR-Cas design

5.3.                10:00-12:00 (C05/312) – Testing of knowledge from online tools used in Gene
Technologies

12.3.             9:00-17:00 (C05/312) – RNA isolation and Restriction and TA-cloning, Gibbson
assembly of specific genes from tomato and Arabidopsis plants (Group 1)

19.3.             9:00-17:00 (C05/312) – RNA isolation and Restriction and TA-cloning, Gibbson
assembly of specific genes from tomato and Arabidopsis plants (Group 2)

26.3.             9:00-17:00 (C05/312) – RNA isolation and Restriction and TA-cloning, Gibbson
assembly of specific genes from tomato and Arabidopsis plants (Group 3)

2.4.                10:00-15:00 (C05/312) – Transient expression of genes in plants: Transformation
of N. benthamiana plants with Agrobacterium tumefaciens (Group1, 2, 3)

9.4.                10:00-15:00 (C05/312) – Analysis of transient expression of the protein in N.
benthamiana: RNA isolation, RT-qPCR analysis (Group1, 2, 3)

16.4.              10:00-15:00 (C05/312) – Analysis of transient expression of the protein in N.
benthamiana: Western-blot analysis

23.4.             9:00-11:00 (C05/312) – Preparation of NGS library (Part 1): Group 1

                        11:00-13:00 (C05/312) - Preparation of NGS library (Part 1): Group 2

                        13:00-15:00 (C05/312) - Preparation of NGS library (Part 1): Group 3

30.4.             9:00-11:00 (C05/312) – Preparation of NGS library (Part 2): Group 1

                        11:00-13:00 (C05/312) - Preparation of NGS library (Part 2): Group 2

                        13:00-15:00 (C05/312) - Preparation of NGS library (Part 2): Group 3