Document Name: kalibracky.sds Plate Type: Absolute Quantification User: 7500RTPCR Document Information Operator: 7500RTPCR Run Date: Tuesday, January 05, 2010 10:24:15 Last Modified: Tuesday, January 05, 2010 12:50:28 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.3.1 Thermal Cycler Profile Stage,Repetitions,Temperature,Time,Ramp Rate,Auto Increment 1,1,50.0 °C,2:00,100,, 2,1,95.0 °C,10:00,100,, 3,40,95.0 °C,0:15,100,, ,,60.0 °C,1:00,100,, Standard 7500 Mode Data Collection : Stage 3 Step 1 PCR Volume: 25 ľL, Well,Sample Name,Detector,Task,Ct,StdDev Ct,Qty,Mean Qty,StdDev Qty,Filtered,Tm A1,25 ng,GAPDH,Unknown,23.42,0.046,,,,, ,,cycD1,Unknown,35.67,0.293,,,,, A2,25 ng,GAPDH,Unknown,23.51,0.046,,,,, ,,cycD1,Unknown,35.42,0.293,,,,, A3,25 ng,GAPDH,Unknown,23.47,0.046,,,,, ,,cycD1,Unknown,36.00,0.293,,,,, A4,ntc,GAPDH,NTC,Undetermined,,,,,, ,,cycD1,NTC,Undetermined,,,,,, A10,100ng,GAPDH,Unknown,23.98,1.602,,,,, ,,cycD1,Unknown,27.05,1.652,,,,, A11,100ng,GAPDH,Unknown,21.54,1.602,,,,, ,,cycD1,Unknown,24.52,1.652,,,,, A12,100ng,GAPDH,Unknown,24.56,1.602,,,,, ,,cycD1,Unknown,27.63,1.652,,,,, B1,2 5 ng,GAPDH,Unknown,27.07,0.073,,,,, ,,cycD1,Unknown,Undetermined,,,,,, B2,2 5 ng,GAPDH,Unknown,27.17,0.073,,,,, ,,cycD1,Unknown,Undetermined,,,,,, B3,2 5 ng,GAPDH,Unknown,27.21,0.073,,,,, ,,cycD1,Unknown,Undetermined,,,,,, B4,ntc,GAPDH,NTC,Undetermined,,,,,, ,,cycD1,NTC,Undetermined,,,,,, B10,10ng,GAPDH,Unknown,22.50,0.395,,,,, ,,cycD1,Unknown,25.99,0.346,,,,, B11,10ng,GAPDH,Unknown,22.22,0.395,,,,, ,,cycD1,Unknown,25.61,0.346,,,,, B12,10ng,GAPDH,Unknown,23.00,0.395,,,,, ,,cycD1,Unknown,26.30,0.346,,,,, C1,0 25 ng,GAPDH,Unknown,30.97,0.139,,,,, ,,cycD1,Unknown,Undetermined,,,,,, C2,0 25 ng,GAPDH,Unknown,30.90,0.139,,,,, ,,cycD1,Unknown,Undetermined,,,,,, C3,0 25 ng,GAPDH,Unknown,30.70,0.139,,,,, ,,cycD1,Unknown,Undetermined,,,,,, C4,ntc,GAPDH,NTC,Undetermined,,,,,, ,,cycD1,NTC,Undetermined,,,,,, C10,1ng,GAPDH,Unknown,25.87,0.131,,,,, ,,cycD1,Unknown,29.18,0.035,,,,, C11,1ng,GAPDH,Unknown,25.70,0.131,,,,, ,,cycD1,Unknown,29.21,0.035,,,,, C12,1ng,GAPDH,Unknown,25.95,0.131,,,,, ,,cycD1,Unknown,29.25,0.035,,,,, D1,25 pg,GAPDH,Unknown,34.37,0.243,,,,, ,,cycD1,Unknown,Undetermined,,,,,, D2,25 pg,GAPDH,Unknown,34.41,0.243,,,,, ,,cycD1,Unknown,Undetermined,,,,,, D3,25 pg,GAPDH,Unknown,34.81,0.243,,,,, ,,cycD1,Unknown,Undetermined,,,,,, D10,100pg,GAPDH,Unknown,29.31,0.128,,,,, ,,cycD1,Unknown,32.71,0.099,,,,, D11,100pg,GAPDH,Unknown,29.09,0.128,,,,, ,,cycD1,Unknown,32.51,0.099,,,,, D12,100pg,GAPDH,Unknown,29.10,0.128,,,,, ,,cycD1,Unknown,32.60,0.099,,,,, E1,2 5 pg,GAPDH,Unknown,38.84,1.121,,,,, ,,cycD1,Unknown,Undetermined,,,,,, E2,2 5 pg,GAPDH,Unknown,38.14,1.121,,,,, ,,cycD1,Unknown,Undetermined,,,,,, E3,2 5 pg,GAPDH,Unknown,36.65,1.121,,,,, ,,cycD1,Unknown,Undetermined,,,,,, E10,10pg,GAPDH,Unknown,32.36,0.227,,,,, ,,cycD1,Unknown,35.72,0.105,,,,, E11,10pg,GAPDH,Unknown,32.45,0.227,,,,, ,,cycD1,Unknown,35.91,0.105,,,,, E12,10pg,GAPDH,Unknown,32.79,0.227,,,,, ,,cycD1,Unknown,35.74,0.105,,,,, F1,0 25 pg,GAPDH,Unknown,Undetermined,,,,,, ,,cycD1,Unknown,Undetermined,,,,,, F2,0 25 pg,GAPDH,Unknown,Undetermined,,,,,, ,,cycD1,Unknown,Undetermined,,,,,, F3,0 25 pg,GAPDH,Unknown,Undetermined,,,,,, ,,cycD1,Unknown,Undetermined,,,,,, F10,1pg,GAPDH,Unknown,35.62,0.270,,,,, ,,cycD1,Unknown,Undetermined,,,,,, F11,1pg,GAPDH,Unknown,36.10,0.270,,,,, ,,cycD1,Unknown,Undetermined,,,,,, F12,1pg,GAPDH,Unknown,36.07,0.270,,,,, ,,cycD1,Unknown,Undetermined,,,,,, G10,ntc,GAPDH,NTC,Undetermined,,,,,, ,,cycD1,NTC,Undetermined,,,,,, G11,ntc,GAPDH,NTC,Undetermined,,,,,, ,,cycD1,NTC,Undetermined,,,,,, G12,ntc,GAPDH,NTC,Undetermined,,,,,, ,,cycD1,NTC,Undetermined,,,,,,