Document Name: sybr.sds 
Plate Type: Absolute Quantification
User: 7500RTPCR

Document Information

Operator: 7500RTPCR
Run Date: Tuesday, January 05, 2010 13:11:54
Last Modified: Tuesday, January 05, 2010 15:08:25
Instrument Type: Applied Biosystems 7500 Real-Time PCR System

Comments:
SDS v1.3.1

Thermal Cycler Profile
Stage,Repetitions,Temperature,Time,Ramp Rate,Auto Increment
1,1,50.0 °C,2:00,100,,
2,1,95.0 °C,10:00,100,,
3,40,95.0 °C,0:15,100,,
,,60.0 °C,1:00,100,,
4 (Dissociation),1,95.0 °C,0:15,Auto,,
,,60.0 °C,1:00,Auto,,
,,95.0 °C,0:15,Auto,,
Standard 7500 Mode
Data Collection :  Stage 3 Step 1
PCR Volume: 25 ľL,

Well,Sample Name,Detector,Task,Ct,StdDev Ct,Qty,Mean Qty,StdDev Qty,Filtered,Tm
A1,,GAPDH_SYBR,Unknown,17.35,,,,,,78.80
A2,,GAPDH_SYBR,Unknown,17.56,,,,,,79.10
A3,,GAPDH_SYBR,Unknown,16.02,,,,,,79.50
A4,,GAPDH_SYBR,Unknown,16.01,,,,,,79.10
A5,,GAPDH_SYBR,Unknown,15.75,,,,,,79.10
A6,,GAPDH_SYBR,Unknown,15.44,,,,,,79.50
B1,,GAPDH_SYBR,Unknown,16.96,,,,,,79.10
B2,,GAPDH_SYBR,Unknown,16.75,,,,,,79.50
B3,,GAPDH_SYBR,Unknown,15.67,,,,,,79.80
B4,,GAPDH_SYBR,Unknown,15.24,,,,,,79.80
B5,,GAPDH_SYBR,Unknown,14.77,,,,,,79.80
B6,,GAPDH_SYBR,Unknown,14.93,,,,,,79.80
C1,,GAPDH_SYBR,Unknown,16.40,,,,,,79.80
C2,,GAPDH_SYBR,Unknown,16.58,,,,,,79.50
C3,,GAPDH_SYBR,Unknown,15.24,,,,,,79.80
C4,,GAPDH_SYBR,Unknown,15.36,,,,,,79.80
C5,,GAPDH_SYBR,Unknown,15.21,,,,,,80.20
C6,,GAPDH_SYBR,Unknown,14.51,,,,,,80.20
D1,,GAPDH_SYBR,Unknown,39.72,,,,,,78.80
D2,,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
D3,,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
D4,,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
D5,,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
D6,,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
E1,,GAPDH_SYBR,Unknown,34.05,,,,,,78.80
E2,,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.50
E3,,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
E4,,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
E5,,GAPDH_SYBR,Unknown,36.83,,,,,,75.30
E6,,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
F1,,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
F2,,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
F3,,GAPDH_SYBR,Unknown,36.00,,,,,,80.20
F4,,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
F5,,GAPDH_SYBR,Unknown,34.74,,,,,,79.80
F6,,GAPDH_SYBR,Unknown,37.44,,,,,,75.30