Document Name: u937.sds 
Plate Type: Absolute Quantification
User: 7500RTPCR

Document Information

Operator: 7500RTPCR
Run Date: Wednesday, January 06, 2010 10:16:23
Last Modified: Wednesday, January 06, 2010 12:04:04
Instrument Type: Applied Biosystems 7500 Real-Time PCR System

Comments:
SDS v1.3.1



Thermal Cycler Profile
Stage,Repetitions,Temperature,Time,Ramp Rate,Auto Increment
1,1,50.0 °C,2:00,100,,
2,1,95.0 °C,10:00,100,,
3,40,95.0 °C,0:15,100,,
,,60.0 °C,1:00,100,,
Standard 7500 Mode
Data Collection :  Stage 3 Step 1
PCR Volume: 25 ľL,

Well,Sample Name,Detector,Task,Ct,StdDev Ct,Qty,Mean Qty,StdDev Qty,Filtered,Tm
A1,u937,cycD1,Unknown,34.28,0.298,,,,,
,,GAPDH,Unknown,26.13,1.209,,,,,
A2,u937,cycD1,Unknown,34.57,0.298,,,,,
,,GAPDH,Unknown,24.11,1.209,,,,,
A3,u937,cycD1,Unknown,33.97,0.298,,,,,
,,GAPDH,Unknown,23.97,1.209,,,,,
A10,zdrava 10ng,cycD1,Unknown,26.76,0.184,,,,,
,,GAPDH,Unknown,21.98,0.095,,,,,
A11,zdrava 10ng,cycD1,Unknown,26.39,0.184,,,,,
,,GAPDH,Unknown,21.81,0.095,,,,,
A12,zdrava 10ng,cycD1,Unknown,26.61,0.184,,,,,
,,GAPDH,Unknown,21.97,0.095,,,,,
B1,u937 tpa,cycD1,Unknown,28.06,0.208,,,,,
,,GAPDH,Unknown,21.21,1.156,,,,,
B2,u937 tpa,cycD1,Unknown,28.20,0.208,,,,,
,,GAPDH,Unknown,19.19,1.156,,,,,
B3,u937 tpa,cycD1,Unknown,27.79,0.208,,,,,
,,GAPDH,Unknown,19.22,1.156,,,,,
B10,nadorova 10 ng,cycD1,Unknown,26.80,0.217,,,,,
,,GAPDH,Unknown,23.25,0.126,,,,,
B11,nadorova 10 ng,cycD1,Unknown,26.66,0.217,,,,,
,,GAPDH,Unknown,23.14,0.126,,,,,
B12,nadorova 10 ng,cycD1,Unknown,26.37,0.217,,,,,
,,GAPDH,Unknown,23.00,0.126,,,,,
C1,nort,cycD1,Unknown,Undetermined,,,,,,
,,GAPDH,Unknown,Undetermined,,,,,,
C2,nort,cycD1,Unknown,Undetermined,,,,,,
,,GAPDH,Unknown,Undetermined,,,,,,
C3,nort,cycD1,Unknown,Undetermined,,,,,,
,,GAPDH,Unknown,Undetermined,,,,,,
C10,ntc,cycD1,NTC,Undetermined,,,,,,
,,GAPDH,NTC,Undetermined,,,,,,
C11,ntc,cycD1,NTC,Undetermined,,,,,,
,,GAPDH,NTC,Undetermined,,,,,,
C12,ntc,cycD1,NTC,Undetermined,,,,,,
,,GAPDH,NTC,Undetermined,,,,,,
D1,u937 25ng,cycD1,Unknown,36.00,,,,,,
,,GAPDH,Unknown,23.97,,,,,,
D2,u937 25ng,cycD1,Unknown,Undetermined,,,,,,
,,GAPDH,Unknown,Undetermined,,,,,,
D3,u937 25ng,cycD1,Unknown,Undetermined,,,,,,
,,GAPDH,Unknown,Undetermined,,,,,,
E1,u937 tpa 25ng,cycD1,Unknown,28.27,0.297,,,,,
,,GAPDH,Unknown,18.66,0.227,,,,,
E2,u937 tpa 25ng,cycD1,Unknown,28.86,0.297,,,,,
,,GAPDH,Unknown,19.05,0.227,,,,,
E3,u937 tpa 25ng,cycD1,Unknown,28.47,0.297,,,,,
,,GAPDH,Unknown,19.06,0.227,,,,,
F1,ntc,cycD1,NTC,Undetermined,,,,,,
,,GAPDH,NTC,Undetermined,,,,,,
F2,ntc,cycD1,NTC,Undetermined,,,,,,
,,GAPDH,NTC,Undetermined,,,,,,
F3,ntc,cycD1,NTC,Undetermined,,,,,,
,,GAPDH,NTC,Undetermined,,,,,,