Document Name: Cvika-sk_1,3-experiment-20.1.2010.sds 
Plate Type: Absolute Quantification
User: 7500RTPCR

Document Information

Operator: 7500RTPCR
Run Date: Wednesday, January 20, 2010 10:34:53
Last Modified: Wednesday, January 20, 2010 12:11:42
Instrument Type: Applied Biosystems 7500 Real-Time PCR System

Comments:
SDS v1.3.1

Thermal Cycler Profile
Stage,Repetitions,Temperature,Time,Ramp Rate,Auto Increment
1,1,50.0 °C,2:00,100,,
2,1,95.0 °C,10:00,100,,
3,40,95.0 °C,0:15,100,,
,,60.0 °C,1:00,100,,
Standard 7500 Mode
Data Collection :  Stage 3 Step 1
PCR Volume: 25 ľL,

Well,Sample Name,Detector,Task,Ct,StdDev Ct,Qty,Mean Qty,StdDev Qty,Filtered,Tm
A1,1.skup U937 +,GAPDH-human,Unknown,23.56,0.104,,,,,
,,cycD1,Unknown,34.98,0.133,,,,,
A2,1.skup U937 +,GAPDH-human,Unknown,23.49,0.104,,,,,
,,cycD1,Unknown,35.00,0.133,,,,,
A3,1.skup U937 +,GAPDH-human,Unknown,23.69,0.104,,,,,
,,cycD1,Unknown,34.76,0.133,,,,,
B1,1.skup U937 -,GAPDH-human,Unknown,20.86,0.136,,,,,
,,cycD1,Unknown,34.29,0.268,,,,,
B2,1.skup U937 -,GAPDH-human,Unknown,21.00,0.136,,,,,
,,cycD1,Unknown,34.37,0.268,,,,,
B3,1.skup U937 -,GAPDH-human,Unknown,21.13,0.136,,,,,
,,cycD1,Unknown,34.79,0.268,,,,,
C1,1.skup NO RT,GAPDH-human,Unknown,29.79,,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
C2,1.skup NO RNA,GAPDH-human,Unknown,Undetermined,,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
C3,1.skup voda,GAPDH-human,Unknown,Undetermined,,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
D1,3skup RPMI s,GAPDH-human,Unknown,17.23,0.044,,,,,
,,Jun,Unknown,27.00,0.205,,,,,
D2,3skup RPMI s,GAPDH-human,Unknown,17.14,0.044,,,,,
,,Jun,Unknown,26.68,0.205,,,,,
D3,3skup RPMI s,GAPDH-human,Unknown,17.21,0.044,,,,,
,,Jun,Unknown,26.62,0.205,,,,,
E1,3skup RPMI r,GAPDH-human,Unknown,16.71,0.264,,,,,
,,Jun,Unknown,25.37,0.176,,,,,
E2,3skup RPMI r,GAPDH-human,Unknown,16.75,0.264,,,,,
,,Jun,Unknown,25.30,0.176,,,,,
E3,3skup RPMI r,GAPDH-human,Unknown,17.19,0.264,,,,,
,,Jun,Unknown,25.64,0.176,,,,,
F1,3skup no rna voda,GAPDH-human,Unknown,Undetermined,,,,,,
,,Jun,Unknown,Undetermined,,,,,,
F2,3skup no rna,GAPDH-human,Unknown,39.07,,,,,,
,,Jun,Unknown,Undetermined,,,,,,
F3,3skup no rt,GAPDH-human,Unknown,34.63,,,,,,
,,Jun,Unknown,29.25,,,,,,