Document Name: Cvika-sk_1	3-experiment-20.1.2010.sds 									
Plate Type: Absolute Quantification										
User: 7500RTPCR										
										
Document Information										
										
Operator: 7500RTPCR										
Run Date: Wednesday	 January 20	 2010 10:34:53								
Last Modified: Wednesday	 January 20	 2010 12:11:42								
Instrument Type: Applied Biosystems 7500 Real-Time PCR System										
										
Comments:										
SDS v1.3.1										
										
Thermal Cycler Profile										
Stage	Repetitions	Temperature	Time	Ramp Rate	Auto Increment					
1	1	50.0 °C	2:00	100						
2	1	95.0 °C	10:00	100						
3	40	95.0 °C	0:15	100						
		60.0 °C	1:00	100						
Standard 7500 Mode										
Data Collection :  Stage 3 Step 1										
PCR Volume: 25 µL										
										
Well	Sample Name	Detector	Task	Ct	StdDev Ct	Qty	Mean Qty	StdDev Qty	Filtered	Tm
A1	1.skup U937 +	GAPDH-human	Unknown	23.56	0.104					
		cycD1	Unknown	34.98	0.133					
A2	1.skup U937 +	GAPDH-human	Unknown	23.49	0.104					
		cycD1	Unknown	35	0.133					
A3	1.skup U937 +	GAPDH-human	Unknown	23.69	0.104					
		cycD1	Unknown	34.76	0.133					
B1	1.skup U937 -	GAPDH-human	Unknown	20.86	0.136					
		cycD1	Unknown	34.29	0.268					
B2	1.skup U937 -	GAPDH-human	Unknown	21	0.136					
		cycD1	Unknown	34.37	0.268					
B3	1.skup U937 -	GAPDH-human	Unknown	21.13	0.136					
		cycD1	Unknown	34.79	0.268					
C1	1.skup NO RT	GAPDH-human	Unknown	29.79						
		cycD1	Unknown	Undetermined						
C2	1.skup NO RNA	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
C3	1.skup voda	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
D1	3skup RPMI s	GAPDH-human	Unknown	17.23	0.044					
		Jun	Unknown	27	0.205					
D2	3skup RPMI s	GAPDH-human	Unknown	17.14	0.044					
		Jun	Unknown	26.68	0.205					
D3	3skup RPMI s	GAPDH-human	Unknown	17.21	0.044					
		Jun	Unknown	26.62	0.205					
E1	3skup RPMI r	GAPDH-human	Unknown	16.71	0.264					
		Jun	Unknown	25.37	0.176					
E2	3skup RPMI r	GAPDH-human	Unknown	16.75	0.264					
		Jun	Unknown	25.3	0.176					
E3	3skup RPMI r	GAPDH-human	Unknown	17.19	0.264					
		Jun	Unknown	25.64	0.176					
F1	3skup no rna voda	GAPDH-human	Unknown	Undetermined						
		Jun	Unknown	Undetermined						
F2	3skup no rna	GAPDH-human	Unknown	39.07						
		Jun	Unknown	Undetermined						
F3	3skup no rt	GAPDH-human	Unknown	34.63						
		Jun	Unknown	29.25						
##### Sheet/List 2 #####

##### Sheet/List 3 #####