Document Name: Cvika-sk_1,3-kalibr._krivka-po_analyze-19.1.2010.sds 
Plate Type: Absolute Quantification
User: 7500RTPCR

Document Information

Operator: 7500RTPCR
Run Date: Tuesday, January 19, 2010 13:38:06
Last Modified: Tuesday, January 19, 2010 15:04:33
Instrument Type: Applied Biosystems 7500 Real-Time PCR System

Comments:
SDS v1.3.1

Thermal Cycler Profile
Stage,Repetitions,Temperature,Time,Ramp Rate,Auto Increment
1,1,50.0 °C,2:00,100,,
2,1,95.0 °C,10:00,100,,
3,40,95.0 °C,0:15,100,,
,,60.0 °C,1:00,100,,
Standard 7500 Mode
Data Collection :  Stage 3 Step 1
PCR Volume: 25 µL,

Well,Sample Name,Detector,Task,Ct,StdDev Ct,Qty,Mean Qty,StdDev Qty,Filtered,Tm
A1,SK.3-RPMIs-100ng,GAPDH-human,Unknown,17.00,0.265,,,,,
,,Jun,Unknown,26.93,0.263,,,,,
A2,SK.3-RPMIs-1ng,GAPDH-human,Unknown,20.52,0.165,,,,,
,,Jun,Unknown,30.00,0.249,,,,,
A3,SK.3-RPMIs-10pg,GAPDH-human,Unknown,24.98,0.051,,,,,
,,Jun,Unknown,34.23,0.271,,,,,
A5,SK.1-U937--100ng,GAPDH-human,Unknown,22.80,0.321,,,,,
,,cycD1,Unknown,36.00,0.840,,,,,
A6,SK.1-U937--1ng,GAPDH-human,Unknown,28.00,0.090,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
A7,SK.1-U937--10pg,GAPDH-human,Unknown,35.26,0.056,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
B1,SK.3-RPMIs-100ng,GAPDH-human,Unknown,16.69,0.265,,,,,
,,Jun,Unknown,26.50,0.263,,,,,
B2,SK.3-RPMIs-1ng,GAPDH-human,Unknown,20.48,0.165,,,,,
,,Jun,Unknown,29.77,0.249,,,,,
B3,SK.3-RPMIs-10pg,GAPDH-human,Unknown,25.04,0.051,,,,,
,,Jun,Unknown,33.69,0.271,,,,,
B5,SK.1-U937--100ng,GAPDH-human,Unknown,22.24,0.321,,,,,
,,cycD1,Unknown,34.61,0.840,,,,,
B6,SK.1-U937--1ng,GAPDH-human,Unknown,28.18,0.090,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
B7,SK.1-U937--10pg,GAPDH-human,Unknown,35.15,0.056,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
C1,SK.3-RPMIs-100ng,GAPDH-human,Unknown,17.22,0.265,,,,,
,,Jun,Unknown,26.45,0.263,,,,,
C2,SK.3-RPMIs-1ng,GAPDH-human,Unknown,20.78,0.165,,,,,
,,Jun,Unknown,29.50,0.249,,,,,
C3,SK.3-RPMIs-10pg,GAPDH-human,Unknown,25.09,0.051,,,,,
,,Jun,Unknown,33.90,0.271,,,,,
C5,SK.1-U937--100ng,GAPDH-human,Unknown,22.78,0.321,,,,,
,,cycD1,Unknown,34.49,0.840,,,,,
C6,SK.1-U937--1ng,GAPDH-human,Unknown,28.06,0.090,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
C7,SK.1-U937--10pg,GAPDH-human,Unknown,35.23,0.056,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
D1,SK.3-RPMIs-10ng,GAPDH-human,Unknown,19.30,0.079,,,,,
,,Jun,Unknown,28.01,0.313,,,,,
D2,SK.3-RPMIs-100pg,GAPDH-human,Unknown,22.87,0.115,,,,,
,,Jun,Unknown,31.21,0.129,,,,,
D3,SK.3-RPMIs-1pg,GAPDH-human,Unknown,26.94,0.148,,,,,
,,Jun,Unknown,34.40,0.574,,,,,
D5,SK.1-U937--10ng,GAPDH-human,Unknown,25.75,0.082,,,,,
,,cycD1,Unknown,38.33,0.194,,,,,
D6,SK.1-U937--100pg,GAPDH-human,Unknown,30.50,0.035,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
D7,SK.1-U937--1pg,GAPDH-human,Unknown,38.08,0.290,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
E1,SK.3-RPMIs-10ng,GAPDH-human,Unknown,19.33,0.079,,,,,
,,Jun,Unknown,28.06,0.313,,,,,
E2,SK.3-RPMIs-100pg,GAPDH-human,Unknown,22.99,0.115,,,,,
,,Jun,Unknown,31.44,0.129,,,,,
E3,SK.3-RPMIs-1pg,GAPDH-human,Unknown,27.23,0.148,,,,,
,,Jun,Unknown,35.00,0.574,,,,,
E5,SK.1-U937--10ng,GAPDH-human,Unknown,25.90,0.082,,,,,
,,cycD1,Unknown,38.05,0.194,,,,,
E6,SK.1-U937--100pg,GAPDH-human,Unknown,30.49,0.035,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
E7,SK.1-U937--1pg,GAPDH-human,Unknown,38.08,0.290,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
F1,SK.3-RPMIs-10ng,GAPDH-human,Unknown,19.18,0.079,,,,,
,,Jun,Unknown,27.49,0.313,,,,,
F2,SK.3-RPMIs-100pg,GAPDH-human,Unknown,23.09,0.115,,,,,
,,Jun,Unknown,31.42,0.129,,,,,
F3,SK.3-RPMIs-1pg,GAPDH-human,Unknown,27.06,0.148,,,,,
,,Jun,Unknown,35.54,0.574,,,,,
F5,SK.1-U937--10ng,GAPDH-human,Unknown,25.77,0.082,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
F6,SK.1-U937--100pg,GAPDH-human,Unknown,30.56,0.035,,,,,
,,cycD1,Unknown,39.34,,,,,,
F7,SK.1-U937--1pg,GAPDH-human,Unknown,37.58,0.290,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
G1,SK.3-RPMIs-NO RT,GAPDH-human,Unknown,34.29,,,,,,
,,Jun,Unknown,28.21,,,,,,
G2,SK.3-RPMIs-NO RNA,GAPDH-human,Unknown,38.99,,,,,,
,,Jun,Unknown,37.99,,,,,,
G5,SK.1-U937--NO RT,GAPDH-human,Unknown,30.25,,,,,,
,,cycD1,Unknown,Undetermined,,,,,,
G6,SK.1-U937--NO RNA,GAPDH-human,Unknown,Undetermined,,,,,,
,,cycD1,Unknown,Undetermined,,,,,,