Document Name: Cvika-sk_2-SYBR_GREEN-optimalizace-po_analyze-19.1.2010.sds 
Plate Type: Absolute Quantification
User: 7500RTPCR

Document Information

Operator: 7500RTPCR
Run Date: Tuesday, January 19, 2010 11:04:43
Last Modified: Tuesday, January 19, 2010 13:11:46
Instrument Type: Applied Biosystems 7500 Real-Time PCR System

Comments:
SDS v1.3.1

Thermal Cycler Profile
Stage,Repetitions,Temperature,Time,Ramp Rate,Auto Increment
1,1,50.0 °C,2:00,100,,
2,1,95.0 °C,10:00,100,,
3,40,95.0 °C,0:15,100,,
,,60.0 °C,1:00,100,,
4 (Dissociation),1,95.0 °C,0:15,Auto,,
,,60.0 °C,1:00,Auto,,
,,95.0 °C,0:15,Auto,,
Standard 7500 Mode
Data Collection :  Stage 3 Step 1
PCR Volume: 25 ľL,

Well,Sample Name,Detector,Task,Ct,StdDev Ct,Qty,Mean Qty,StdDev Qty,Filtered,Tm
A1,F*0.5_R*0.5,SYBR green,Unknown,14.00,0.073,,,,,79.50
A2,F*0.5_R*0.5,SYBR green,Unknown,14.10,0.073,,,,,79.50
A3,F*0.5_R1.5,SYBR green,Unknown,12.91,0.082,,,,,79.80
A4,F*0.5_R1.5,SYBR green,Unknown,13.03,0.082,,,,,79.80
A5,F*0.5_R4.5,SYBR green,Unknown,12.69,0.175,,,,,79.80
A6,F*0.5_R4.5,SYBR green,Unknown,12.94,0.175,,,,,79.80
B1,F1.5_R*0.5,SYBR green,Unknown,13.63,0.096,,,,,79.80
B2,F1.5_R*0.5,SYBR green,Unknown,13.77,0.096,,,,,79.80
B3,F1.5_R1.5,SYBR green,Unknown,12.46,0.068,,,,,80.20
B4,F1.5_R1.5,SYBR green,Unknown,12.37,0.068,,,,,80.20
B5,F1.5_R4.5,SYBR green,Unknown,12.16,1.195,,,,,80.20
B6,F1.5_R4.5,SYBR green,Unknown,10.47,1.195,,,,,80.20
C1,F4.5_R*0.5,SYBR green,Unknown,13.67,0.052,,,,,79.80
C2,F4.5_R*0.5,SYBR green,Unknown,13.59,0.052,,,,,80.20
C3,F4.5_R1.5,SYBR green,Unknown,12.46,0.180,,,,,80.20
C4,F4.5_R1.5,SYBR green,Unknown,12.21,0.180,,,,,80.20
C5,F4.5_R4.5,SYBR green,Unknown,13.84,2.330,,,,,79.20
C6,F4.5_R4.5,SYBR green,Unknown,10.54,2.330,,,,,80.50
D1,F*0.5_R*0.5,SYBR green,NTC,34.60,0.306,,,,,79.20
D2,F*0.5_R*0.5,SYBR green,NTC,34.17,0.306,,,,,79.50
D3,F*0.5_R1.5,SYBR green,NTC,32.60,0.510,,,,,79.50
D4,F*0.5_R1.5,SYBR green,NTC,31.88,0.510,,,,,79.50
D5,F*0.5_R4.5,SYBR green,NTC,Undetermined,,,,,,83.90
D6,F*0.5_R4.5,SYBR green,NTC,32.45,,,,,,79.50
E1,F1.5_R*0.5,SYBR green,NTC,33.62,0.595,,,,,79.50
E2,F1.5_R*0.5,SYBR green,NTC,32.78,0.595,,,,,79.50
E3,F1.5_R1.5,SYBR green,NTC,31.66,1.484,,,,,79.80
E4,F1.5_R1.5,SYBR green,NTC,29.56,1.484,,,,,79.80
E5,F1.5_R4.5,SYBR green,NTC,32.21,0.046,,,,,79.50
E6,F1.5_R4.5,SYBR green,NTC,32.27,0.046,,,,,79.50
F1,F4.5_R*0.5,SYBR green,NTC,33.19,0.605,,,,,79.20
F2,F4.5_R*0.5,SYBR green,NTC,32.33,0.605,,,,,79.50
F3,F4.5_R1.5,SYBR green,NTC,31.05,0.069,,,,,79.80
F4,F4.5_R1.5,SYBR green,NTC,31.14,0.069,,,,,79.80
F5,F4.5_R4.5,SYBR green,NTC,31.80,0.068,,,,,79.80
F6,F4.5_R4.5,SYBR green,NTC,31.90,0.068,,,,,79.80
G1,F2_R*2 - noRT,SYBR green,NTC,20.32,0.100,,,,,79.50
G2,F2_R*2 - noRT,SYBR green,NTC,20.18,0.100,,,,,79.80
G3,F*2_R2 - no RNA,SYBR green,NTC,30.03,0.142,,,,,79.80
G4,F*2_R2 - no RNA,SYBR green,NTC,30.23,0.142,,,,,79.80