Document Name: Cvika-sk_2-experiment-20.1.2010.sds 
Plate Type: Absolute Quantification
User: 7500RTPCR

Document Information

Operator: 7500RTPCR
Run Date: Wednesday, January 20, 2010 12:33:37
Last Modified: Wednesday, January 20, 2010 16:34:57
Instrument Type: Applied Biosystems 7500 Real-Time PCR System

Comments:
SDS v1.3.1

Thermal Cycler Profile
Stage,Repetitions,Temperature,Time,Ramp Rate,Auto Increment
1,1,50.0 °C,2:00,100,,
2,1,95.0 °C,10:00,100,,
3,40,95.0 °C,0:15,100,,
,,60.0 °C,1:00,100,,
4 (Dissociation),1,95.0 °C,0:15,Auto,,
,,60.0 °C,1:00,Auto,,
,,95.0 °C,0:15,Auto,,
Standard 7500 Mode
Data Collection :  Stage 3 Step 1
PCR Volume: 25 µL,

Well,Sample Name,Detector,Task,Ct,StdDev Ct,Qty,Mean Qty,StdDev Qty,Filtered,Tm
A1,N - 100ng,GAPDH_SYBR,Unknown,16.53,0.068,,,,,81.10
A2,N - 100ng,GAPDH_SYBR,Unknown,16.57,0.068,,,,,81.10
A3,N - 100ng,GAPDH_SYBR,Unknown,16.44,0.068,,,,,81.10
A4,N - 10ng,GAPDH_SYBR,Unknown,20.82,0.134,,,,,80.70
A5,N - 10ng,GAPDH_SYBR,Unknown,20.56,0.134,,,,,80.70
A6,N - 10ng,GAPDH_SYBR,Unknown,20.67,0.134,,,,,81.10
A7,N - 1ng,GAPDH_SYBR,Unknown,23.65,0.135,,,,,80.70
A8,N - 1ng,GAPDH_SYBR,Unknown,23.66,0.135,,,,,80.70
A9,N - 1ng,GAPDH_SYBR,Unknown,23.89,0.135,,,,,80.40
A10,N - 100pg,GAPDH_SYBR,Unknown,26.43,0.140,,,,,80.40
A11,N - 100pg,GAPDH_SYBR,Unknown,26.56,0.140,,,,,80.40
A12,N - 100pg,GAPDH_SYBR,Unknown,26.71,0.140,,,,,80.40
B1,N - 10pg,GAPDH_SYBR,Unknown,30.00,0.321,,,,,80.70
B2,N - 10pg,GAPDH_SYBR,Unknown,29.89,0.321,,,,,80.70
B3,N - 10pg,GAPDH_SYBR,Unknown,29.40,0.321,,,,,81.10
B4,N - 1pg,GAPDH_SYBR,Unknown,33.53,0.733,,,,,80.70
B5,N - 1pg,GAPDH_SYBR,Unknown,33.28,0.733,,,,,80.70
B6,N - 1pg,GAPDH_SYBR,Unknown,32.15,0.733,,,,,80.70
B7,N - NO RT,GAPDH_SYBR,Unknown,19.01,0.474,,,,,81.50
B8,N - NO RT,GAPDH_SYBR,Unknown,19.68,0.474,,,,,81.10
B9,N - NO RNA,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
B10,N - NO RNA,GAPDH_SYBR,Unknown,38.34,,,,,,75.00
C1,E - 100ng,GAPDH_SYBR,Unknown,Undetermined,,,,,,78.90
C2,E - 100ng,GAPDH_SYBR,Unknown,Undetermined,,,,,,78.90
C3,E - 100ng,GAPDH_SYBR,Unknown,28.64,,,,,,79.30
C4,E - 10ng,GAPDH_SYBR,Unknown,Undetermined,,,,,,78.60
C5,E - 10ng,GAPDH_SYBR,Unknown,27.47,1.008,,,,,78.90
C6,E - 10ng,GAPDH_SYBR,Unknown,26.05,1.008,,,,,78.90
C7,E - 1ng,GAPDH_SYBR,Unknown,29.19,,,,,,78.90
C8,E - 1ng,GAPDH_SYBR,Unknown,Undetermined,,,,,,78.90
C9,E - 1ng,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
C10,E - 100pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
C11,E - 100pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
C12,E - 100pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
D1,E - 10pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
D2,E - 10pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
D3,E - 10pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
D4,E - 1pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
D5,E - 1pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,79.30
D6,E - 1pg,GAPDH_SYBR,Unknown,38.66,,,,,,73.60
D7,E - NO RT,GAPDH_SYBR,Unknown,25.83,1.900,,,,,78.20
D8,E - NO RT,GAPDH_SYBR,Unknown,28.52,1.900,,,,,78.60
D9,E - NO RNA,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
D10,E - NO RNA,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
E1,D - 100ng,GAPDH_SYBR,Unknown,Undetermined,,,,,,74.60
E2,D - 100ng,GAPDH_SYBR,Unknown,Undetermined,,,,,,75.00
E3,D - 100ng,GAPDH_SYBR,Unknown,Undetermined,,,,,,75.40
E4,D - 10ng,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
E5,D - 10ng,GAPDH_SYBR,Unknown,Undetermined,,,,,,74.60
E6,D - 10ng,GAPDH_SYBR,Unknown,Undetermined,,,,,,75.00
E7,D - 1ng,GAPDH_SYBR,Unknown,Undetermined,,,,,,75.00
E8,D - 1ng,GAPDH_SYBR,Unknown,Undetermined,,,,,,75.00
E9,D - 1ng,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
E10,D - 100pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
E11,D - 100pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
E12,D - 100pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,67.50
F1,D - 10pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
F2,D - 10pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
F3,D - 10pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.80
F4,D - 1pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,67.20
F5,D - 1pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
F6,D - 1pg,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
F7,D - NO RT,GAPDH_SYBR,Unknown,Undetermined,,,,,,73.90
F8,D - NO RT,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
F9,D - NO RNA,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10
F10,D - NO RNA,GAPDH_SYBR,Unknown,Undetermined,,,,,,66.10