Document Name: Cvika-sk_2-experiment-20.1.2010.sds Plate Type: Absolute Quantification User: 7500RTPCR Document Information Operator: 7500RTPCR Run Date: Wednesday January 20 2010 12:33:37 Last Modified: Wednesday January 20 2010 16:34:57 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.3.1 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate Auto Increment 1 1 50.0 °C 2:00 100 2 1 95.0 °C 10:00 100 3 40 95.0 °C 0:15 100 60.0 °C 1:00 100 4 (Dissociation) 1 95.0 °C 0:15 Auto 60.0 °C 1:00 Auto 95.0 °C 0:15 Auto Standard 7500 Mode Data Collection : Stage 3 Step 1 PCR Volume: 25 µL Well Sample Name Detector Task Ct StdDev Ct Qty Mean Qty StdDev Qty Filtered Tm A1 N - 100ng GAPDH_SYBR Unknown 16.53 0.068 81.1 A2 N - 100ng GAPDH_SYBR Unknown 16.57 0.068 81.1 A3 N - 100ng GAPDH_SYBR Unknown 16.44 0.068 81.1 A4 N - 10ng GAPDH_SYBR Unknown 20.82 0.134 80.7 A5 N - 10ng GAPDH_SYBR Unknown 20.56 0.134 80.7 A6 N - 10ng GAPDH_SYBR Unknown 20.67 0.134 81.1 A7 N - 1ng GAPDH_SYBR Unknown 23.65 0.135 80.7 A8 N - 1ng GAPDH_SYBR Unknown 23.66 0.135 80.7 A9 N - 1ng GAPDH_SYBR Unknown 23.89 0.135 80.4 A10 N - 100pg GAPDH_SYBR Unknown 26.43 0.14 80.4 A11 N - 100pg GAPDH_SYBR Unknown 26.56 0.14 80.4 A12 N - 100pg GAPDH_SYBR Unknown 26.71 0.14 80.4 B1 N - 10pg GAPDH_SYBR Unknown 30 0.321 80.7 B2 N - 10pg GAPDH_SYBR Unknown 29.89 0.321 80.7 B3 N - 10pg GAPDH_SYBR Unknown 29.4 0.321 81.1 B4 N - 1pg GAPDH_SYBR Unknown 33.53 0.733 80.7 B5 N - 1pg GAPDH_SYBR Unknown 33.28 0.733 80.7 B6 N - 1pg GAPDH_SYBR Unknown 32.15 0.733 80.7 B7 N - NO RT GAPDH_SYBR Unknown 19.01 0.474 81.5 B8 N - NO RT GAPDH_SYBR Unknown 19.68 0.474 81.1 B9 N - NO RNA GAPDH_SYBR Unknown Undetermined 66.1 B10 N - NO RNA GAPDH_SYBR Unknown 38.34 75 C1 E - 100ng GAPDH_SYBR Unknown Undetermined 78.9 C2 E - 100ng GAPDH_SYBR Unknown Undetermined 78.9 C3 E - 100ng GAPDH_SYBR Unknown 28.64 79.3 C4 E - 10ng GAPDH_SYBR Unknown Undetermined 78.6 C5 E - 10ng GAPDH_SYBR Unknown 27.47 1.008 78.9 C6 E - 10ng GAPDH_SYBR Unknown 26.05 1.008 78.9 C7 E - 1ng GAPDH_SYBR Unknown 29.19 78.9 C8 E - 1ng GAPDH_SYBR Unknown Undetermined 78.9 C9 E - 1ng GAPDH_SYBR Unknown Undetermined 66.1 C10 E - 100pg GAPDH_SYBR Unknown Undetermined 66.1 C11 E - 100pg GAPDH_SYBR Unknown Undetermined 66.1 C12 E - 100pg GAPDH_SYBR Unknown Undetermined 66.1 D1 E - 10pg GAPDH_SYBR Unknown Undetermined 66.1 D2 E - 10pg GAPDH_SYBR Unknown Undetermined 66.1 D3 E - 10pg GAPDH_SYBR Unknown Undetermined 66.1 D4 E - 1pg GAPDH_SYBR Unknown Undetermined 66.1 D5 E - 1pg GAPDH_SYBR Unknown Undetermined 79.3 D6 E - 1pg GAPDH_SYBR Unknown 38.66 73.6 D7 E - NO RT GAPDH_SYBR Unknown 25.83 1.9 78.2 D8 E - NO RT GAPDH_SYBR Unknown 28.52 1.9 78.6 D9 E - NO RNA GAPDH_SYBR Unknown Undetermined 66.1 D10 E - NO RNA GAPDH_SYBR Unknown Undetermined 66.1 E1 D - 100ng GAPDH_SYBR Unknown Undetermined 74.6 E2 D - 100ng GAPDH_SYBR Unknown Undetermined 75 E3 D - 100ng GAPDH_SYBR Unknown Undetermined 75.4 E4 D - 10ng GAPDH_SYBR Unknown Undetermined 66.1 E5 D - 10ng GAPDH_SYBR Unknown Undetermined 74.6 E6 D - 10ng GAPDH_SYBR Unknown Undetermined 75 E7 D - 1ng GAPDH_SYBR Unknown Undetermined 75 E8 D - 1ng GAPDH_SYBR Unknown Undetermined 75 E9 D - 1ng GAPDH_SYBR Unknown Undetermined 66.1 E10 D - 100pg GAPDH_SYBR Unknown Undetermined 66.1 E11 D - 100pg GAPDH_SYBR Unknown Undetermined 66.1 E12 D - 100pg GAPDH_SYBR Unknown Undetermined 67.5 F1 D - 10pg GAPDH_SYBR Unknown Undetermined 66.1 F2 D - 10pg GAPDH_SYBR Unknown Undetermined 66.1 F3 D - 10pg GAPDH_SYBR Unknown Undetermined 66.8 F4 D - 1pg GAPDH_SYBR Unknown Undetermined 67.2 F5 D - 1pg GAPDH_SYBR Unknown Undetermined 66.1 F6 D - 1pg GAPDH_SYBR Unknown Undetermined 66.1 F7 D - NO RT GAPDH_SYBR Unknown Undetermined 73.9 F8 D - NO RT GAPDH_SYBR Unknown Undetermined 66.1 F9 D - NO RNA GAPDH_SYBR Unknown Undetermined 66.1 F10 D - NO RNA GAPDH_SYBR Unknown Undetermined 66.1 ##### Sheet/List 2 ##### ##### Sheet/List 3 #####