Document Name: IJaMK 24.11 dopo SYBR.sds 										
Plate Type: Absolute Quantification										
User: 7500RTPCR										
										
Document Information										
										
Operator: zdenek										
Run Date: Wednesday	 November 24	 2010 13:22:37								
Last Modified: Wednesday	 November 24	 2010 15:32:23								
Instrument Type: Applied Biosystems 7500 Real-Time PCR System										
										
Comments:										
SDS v1.3.1										
										
Thermal Cycler Profile										
Stage	Repetitions	Temperature	Time	Ramp Rate	Auto Increment					
1	1	50.0 °C	2:00	100						
2	1	95.0 °C	10:00	100						
3	40	95.0 °C	0:15	100						
		60.0 °C	1:00	100						
4 (Dissociation)	1	95.0 °C	0:15	Auto						
		60.0 °C	1:00	Auto						
		95.0 °C	0:15	Auto						
Standard 7500 Mode										
Data Collection :  Stage 3 Step 1										
PCR Volume: 25 µL										
										
Well	Sample Name	Detector	Task	Ct	StdDev Ct	Qty	Mean Qty	StdDev Qty	Filtered	Tm
A1	"cDNA 42 ul2,5"	SYBRgreen	Unknown	19.6						78.2
A2	cDNA 42	SYBRgreen	Unknown	21.84	0.746					79.6
A3	cDNA 42	SYBRgreen	Unknown	20.78	0.746					80
A4	NO 42	SYBRgreen	Unknown	22.84	0.189					79.6
A5	NO 42	SYBRgreen	Unknown	22.85	0.189					79.3
A6	NO 42	SYBRgreen	Unknown	22.52	0.189					79.6
B1	cDNA42	GAPDH	Unknown	30.52	0.259					84.6
		cycD1	Unknown	34.54	0.322					79.3
B2	cDNA42	GAPDH	Unknown	Undetermined						63.2
		cycD1	Unknown	Undetermined						71.5
B3	cDNA42	GAPDH	Unknown	30.89	0.259					63.2
		cycD1	Unknown	35	0.322					83.5
B4	NO 42	GAPDH	Unknown	Undetermined						63.2
		cycD1	Unknown	Undetermined						70.8
B5	NO 42	GAPDH	Unknown	Undetermined						63.2
		cycD1	Unknown	Undetermined						79.6
B6	NO 42	GAPDH	Unknown	Undetermined						63.2
		cycD1	Unknown	Undetermined						72.6
C1	no template	SYBRgreen	Unknown	35.33	0.9					80.3
C2	no template	SYBRgreen	Unknown	34.22	0.9					80.3
C3	no template	SYBRgreen	Unknown	36	0.9					75.4
C4	no template	GAPDH	Unknown	Undetermined						63.2
		cycD1	Unknown	Undetermined						76.1
C5	no template	GAPDH	Unknown	Undetermined						63.2
		cycD1	Unknown	Undetermined						79.3
C6	no template	GAPDH	Unknown	Undetermined						63.2
		cycD1	Unknown	Undetermined						80.7