Document Name: Lenka_Gabka 24.11.dopo.sds 										
Plate Type: Absolute Quantification										
User: 7500RTPCR										
										
Document Information										
										
Operator: zdenek										
Run Date: Wednesday	 November 24	 2010 15:53:31								
Last Modified: Wednesday	 November 24	 2010 17:13:47								
Instrument Type: Applied Biosystems 7500 Real-Time PCR System										
										
Comments:										
SDS v1.3.1										
										
Thermal Cycler Profile										
Stage	Repetitions	Temperature	Time	Ramp Rate	Auto Increment					
1	1	50.0 °C	2:00	100						
2	1	95.0 °C	10:00	100						
3	40	95.0 °C	0:15	100						
		60.0 °C	1:00	100						
Standard 7500 Mode										
Data Collection :  Stage 3 Step 1										
PCR Volume: 25 µL										
										
Well	Sample Name	Detector	Task	Ct	StdDev Ct	Qty	Mean Qty	StdDev Qty	Filtered	Tm
A1	NTC L	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A2	NTC	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A3	NTC	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A4	NO RT	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A5	NO RT	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A6	NO RT	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A7	cDNA	GAPDH	Unknown	19.6	0.875					
		cycD1	Unknown	31	0.441					
A8	cDNA	GAPDH	Unknown	19.61	0.875					
		cycD1	Unknown	30.99	0.441					
A9	cDNA	GAPDH	Unknown	20.07	0.875					
		cycD1	Unknown	31.35	0.441					
A10	"EtOH 0,5"	GAPDH	Unknown	31.51	5.307					
		cycD1	Unknown	Undetermined						
A11	"EtOH 0,5"	GAPDH	Unknown	24	5.307					
		cycD1	Unknown	Undetermined						
A12	pouze MM	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
B1	pouze MM	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
B2	pouze MM	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
B3	pouze MM	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
C1	NTC G	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
C2	NTC	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
C3	NTC	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
C4	NO RT	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
C5	NO RT	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
C6	NO RT	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
C7	cDNA	GAPDH	Unknown	21.16	0.875					
		cycD1	Unknown	30.29	0.441					
C8	cDNA	GAPDH	Unknown	21.34	0.875					
		cycD1	Unknown	30.35	0.441					
C9	cDNA	GAPDH	Unknown	21.44	0.875					
		cycD1	Unknown	30.39	0.441					
C10	pouze MM	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
C11	pouze MM	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
C12	pouze MM	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
D1	EtOH 1	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
D2	EtOH 1	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
D3	pouze MM	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined