Document Name: SilviaZuzka 3.skupina 24.11.2010.sds 										
Plate Type: Absolute Quantification										
User: 7500RTPCR										
										
Document Information										
										
Operator: 7500RTPCR										
Run Date: Wednesday	 November 24	 2010 11:15:09								
Last Modified: Wednesday	 November 24	 2010 12:50:18								
Instrument Type: Applied Biosystems 7500 Real-Time PCR System										
										
Comments:										
SDS v1.3.1										
										
Thermal Cycler Profile										
Stage	Repetitions	Temperature	Time	Ramp Rate	Auto Increment					
1	1	50.0 °C	2:00	100						
2	1	95.0 °C	10:00	100						
3	40	95.0 °C	0:15	100						
		60.0 °C	1:00	100						
Standard 7500 Mode										
Data Collection :  Stage 3 Step 1										
PCR Volume: 25 µL										
										
Well	Sample Name	Detector	Task	Ct	StdDev Ct	Qty	Mean Qty	StdDev Qty	Filtered	Tm
A1	Z ntc	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A2	Z ntc	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A3	Z ntc	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A4	Z cDNA	GAPDH-human	Unknown	18.49	0.14					
		cycD1	Unknown	22.23	0.084					
A5	Z cDNA	GAPDH-human	Unknown	18.69	0.14					
		cycD1	Unknown	22.34	0.084					
A6	Z 0	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A7	Z 1ul EtOH	GAPDH-human	Unknown	19	0.002					
		cycD1	Unknown	22.43	0.07					
A8	Z 1ul EtOH	GAPDH-human	Unknown	19	0.002					
		cycD1	Unknown	22.33	0.07					
A9	Z0	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A10	"Z 0,5ul EtOH"	GAPDH-human	Unknown	18.98						
		cycD1	Unknown	22.36						
A11	Z 0	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A12	Z 0	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
B1	S ntc	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
B2	S ntc	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
B3	S ntc	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
B4	S cDNA	GAPDH-human	Unknown	21.34	2.367					
		cycD1	Unknown	25.41	2.555					
B5	S cDNA	GAPDH-human	Unknown	24.69	2.367					
		cycD1	Unknown	29.03	2.555					
B6	S 0	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
B7	S 1ul EtOH	GAPDH-human	Unknown	22.14	0.11					
		cycD1	Unknown	26	0.146					
B8	S 1ul EtOH	GAPDH-human	Unknown	21.98	0.11					
		cycD1	Unknown	25.79	0.146					
B9	S0	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
B10	"S 0,5 EtOH"	GAPDH-human	Unknown	21.74						
		cycD1	Unknown	25.64						
B11	"S 0,5ul EtOH 1,5ul cDNA"	GAPDH-human	Unknown	22.96						
		cycD1	Unknown	26.74						
B12	S 0	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined