Document Name: SilviaZuzana analyzed.sds Plate Type: Standard Curve User: sevcikova Document Information Operator: Administrator Run Date: Tuesday November 23 2010 09:47:43 Last Modified: Tuesday November 23 2010 13:27:23 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.4 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate Auto Increment 1 1 50.0 °C 2:00 100 2 1 95.0 °C 10:00 100 3 40 95.0 °C 0:15 100 60.0 °C 1:00 100 Standard 7500 Mode Data Collection : Stage 3 Step 2 PCR Volume: 25 µL Well Sample Name Detector Task Ct StdDev Ct Qty Mean Qty StdDev Qty Filtered Tm User Defined #1 User Defined #2 User Defined #3 A1 NTC Sylvia GAPDH_VIC Unknown Undetermined A1 NTC Sylvia DKK1 Unknown Undetermined A2 NTC Sylvia GAPDH_VIC Unknown Undetermined A2 NTC Sylvia DKK1 Unknown Undetermined A3 NTC Sylvia GAPDH_VIC Unknown Undetermined A3 NTC Sylvia DKK1 Unknown Undetermined A4 cdna GAPDH_VIC Unknown 20.8056 0.153 A4 cdna DKK1 Unknown 24.5405 0.243 A5 cdna GAPDH_VIC Unknown 20.8031 0.153 A5 cdna DKK1 Unknown 24.5794 0.243 A6 cdna GAPDH_VIC Unknown 21.0695 0.153 A6 cdna DKK1 Unknown 24.98 0.243 A7 10x GAPDH_VIC Unknown 23.9224 1.38 A7 10x DKK1 Unknown 28.085 1.63 A8 10x GAPDH_VIC Unknown 23.3782 1.38 A8 10x DKK1 Unknown 27.5319 1.63 A9 10x GAPDH_VIC Unknown 23.593 1.38 A9 10x DKK1 Unknown 27.7016 1.63 A10 100x GAPDH_VIC Unknown 27.424 1.4 A10 100x DKK1 Unknown 31.4838 1.71 A11 100x GAPDH_VIC Unknown 27.262 1.4 A11 100x DKK1 Unknown 31.4024 1.71 A12 100x GAPDH_VIC Unknown 27.3115 1.4 A12 100x DKK1 Unknown 31.5383 1.71 B1 1000x GAPDH_VIC Unknown 31.0089 1.3 B1 1000x DKK1 Unknown 34.9926 1.69 B2 1000x GAPDH_VIC Unknown 31.4551 1.3 B2 1000x DKK1 Unknown 35.2997 1.69 B3 1000x GAPDH_VIC Unknown 30.6826 1.3 B3 1000x DKK1 Unknown 35.2117 1.69 B4 10 000x GAPDH_VIC Unknown 34.4055 1.38 B4 10 000x DKK1 Unknown 39.1158 2.01 B5 10 000x GAPDH_VIC Unknown 33.8925 1.38 B5 10 000x DKK1 Unknown Undetermined 2.01 B6 10 000x GAPDH_VIC Unknown 34.6214 1.38 B6 10 000x DKK1 Unknown Undetermined 2.01 B7 100 000x GAPDH_VIC Unknown 37.1401 2.02 B7 100 000x DKK1 Unknown Undetermined 0.823 B8 100 000x GAPDH_VIC Unknown 37.7851 2.02 B8 100 000x DKK1 Unknown Undetermined 0.823 B9 100 000x GAPDH_VIC Unknown 39.4672 2.02 B9 100 000x DKK1 Unknown Undetermined 0.823 G1 1000x GAPDH_VIC Unknown 28.8612 1.3 G1 1000x DKK1 Unknown 32.1557 1.69 G3 1000x GAPDH_VIC Unknown 28.6978 1.3 G3 1000x DKK1 Unknown 32.0168 1.69 G4 1000x GAPDH_VIC Unknown 28.6004 1.3 G4 1000x DKK1 Unknown 32.0772 1.69 G5 10 000x GAPDH_VIC Unknown 31.8912 1.38 G5 10 000x DKK1 Unknown 35.9119 2.01 G6 10 000x GAPDH_VIC Unknown 31.9205 1.38 G6 10 000x DKK1 Unknown 35 2.01 G7 10 000x GAPDH_VIC Unknown 31.6641 1.38 G7 10 000x DKK1 Unknown 34.7619 2.01 G8 100 000x GAPDH_VIC Unknown 34.6289 2.02 G8 100 000x DKK1 Unknown 39.338 0.823 G9 100 000x GAPDH_VIC Unknown 34.5619 2.02 G9 100 000x DKK1 Unknown 38.5139 0.823 G10 100 000x GAPDH_VIC Unknown 35 2.02 G10 100 000x DKK1 Unknown 37.6914 0.823 H1 NTC Zuzana GAPDH_VIC Unknown Undetermined H1 NTC Zuzana DKK1 Unknown Undetermined H2 NTC Zuzana GAPDH_VIC Unknown Undetermined H2 NTC Zuzana DKK1 Unknown Undetermined H3 NTC Zuzana GAPDH_VIC Unknown Undetermined H3 NTC Zuzana DKK1 Unknown Undetermined H4 cDNA GAPDH_VIC Unknown 17.5801 0.0618 H4 cDNA DKK1 Unknown 21.39 0.137 H5 cDNA GAPDH_VIC Unknown 17.6475 0.0618 H5 cDNA DKK1 Unknown 21.4153 0.137 H6 cDNA GAPDH_VIC Unknown 17.7035 0.0618 H6 cDNA DKK1 Unknown 21.6383 0.137 H7 10x GAPDH_VIC Unknown 20.9712 1.38 H7 10x DKK1 Unknown 24.6345 1.63 H8 10x GAPDH_VIC Unknown 21.1584 1.38 H8 10x DKK1 Unknown 24.8652 1.63 H9 10x GAPDH_VIC Unknown 21.2599 1.38 H9 10x DKK1 Unknown 24.9716 1.63 H10 100x GAPDH_VIC Unknown 24.8231 1.4 H10 100x DKK1 Unknown 28.3315 1.71 H11 100x GAPDH_VIC Unknown 24.8548 1.4 H11 100x DKK1 Unknown 28.4034 1.71 H12 100x GAPDH_VIC Unknown 24.6798 1.4 H12 100x DKK1 Unknown 28.3306 1.71