Document Name: skupina 2. - odpo. 23.11.2010.sds 										
Plate Type: Absolute Quantification										
User: 7500RTPCR										
										
Document Information										
										
Operator: 7500RTPCR										
Run Date: Tuesday	 November 23	 2010 14:59:10								
Last Modified: Tuesday	 November 23	 2010 17:02:07								
Instrument Type: Applied Biosystems 7500 Real-Time PCR System										
										
Comments:										
SDS v1.3.1										
										
Thermal Cycler Profile										
Stage	Repetitions	Temperature	Time	Ramp Rate	Auto Increment					
1	1	50.0 °C	2:00	100						
2	1	95.0 °C	10:00	100						
3	40	95.0 °C	0:15	100						
		60.0 °C	1:00	100						
4 (Dissociation)	1	95.0 °C	0:15	Auto						
		60.0 °C	1:00	Auto						
		95.0 °C	0:15	Auto						
Standard 7500 Mode										
Data Collection :  Stage 3 Step 1										
PCR Volume: 25 µL										
										
Well	Sample Name	Detector	Task	Ct	StdDev Ct	Qty	Mean Qty	StdDev Qty	Filtered	Tm
A1	0.5* 0.5*	GAPDH_SYBR	Unknown	Undetermined						68.5
A2	0.5* 0.5*	GAPDH_SYBR	Unknown	Undetermined						66.8
A3	0.5* 1.5	GAPDH_SYBR	Unknown	17.1	0.238					79.4
A4	0.5* 1.5	GAPDH_SYBR	Unknown	16.76	0.238					79.4
A5	0.5* 4.5	GAPDH_SYBR	Unknown	Undetermined						66.1
A6	0.5* 4.5	GAPDH_SYBR	Unknown	17.18						79.4
B1	1.5 0.5*	GAPDH_SYBR	Unknown	16.86	0.123					79.8
B2	1.5 0.5*	GAPDH_SYBR	Unknown	17.03	0.123					79.8
B3	1.5 1.5	GAPDH_SYBR	Unknown	33.5	12.016					75.3
B4	1.5 1.5	GAPDH_SYBR	Unknown	16.51	12.016					79.8
B5	1.5 4.5	GAPDH_SYBR	Unknown	16.91	0.198					80.1
B6	1.5 4.5	GAPDH_SYBR	Unknown	17.19	0.198					79.8
C1	4.5 0.5*	GAPDH_SYBR	Unknown	16.77	0.072					79.8
C2	4.5 0.5*	GAPDH_SYBR	Unknown	16.87	0.072					79.8
C3	4.5 1.5	GAPDH_SYBR	Unknown	16.53	0.064					80.1
C4	4.5 1.5	GAPDH_SYBR	Unknown	16.44	0.064					80.1
C5	4.5 4.5	GAPDH_SYBR	Unknown	16.66	0.033					80.1
C6	4.5 4.5	GAPDH_SYBR	Unknown	16.62	0.033					80.1
D1	0.5* 0.5* k	GAPDH_SYBR	Unknown	34.6	3.222					80.5
D2	0.5* 0.5* k	GAPDH_SYBR	Unknown	39.16	3.222					75.7
D3	0.5* 1.5 k	GAPDH_SYBR	Unknown	Undetermined						66.1
D4	0.5* 1.5 k	GAPDH_SYBR	Unknown	35.57	1.325					80.5
D5	0.5* 1.5 k	GAPDH_SYBR	Unknown	33.69	1.325					76
D6	0.5* 4.5 k	GAPDH_SYBR	Unknown	34.31						75.7
E1	1.5 0.5* k	GAPDH_SYBR	Unknown	32.82	2.246					80.1
E2	1.5 0.5* k	GAPDH_SYBR	Unknown	36	2.246					80.1
E3	1.5 1.5 k	GAPDH_SYBR	Unknown	34.55	3.095					79.8
E4	1.5 1.5 k	GAPDH_SYBR	Unknown	38.93	3.095					74.6
E5	1.5 4.5 k	GAPDH_SYBR	Unknown	33.47	0.672					76
E6	1.5 4.5 k	GAPDH_SYBR	Unknown	32.52	0.672					75.7
F1	4.5 0.5* k	GAPDH_SYBR	Unknown	34.63	0.426					75.7
F2	4.5 0.5* k	GAPDH_SYBR	Unknown	35.24	0.426					75.7
F3	4.5 1.5 k	GAPDH_SYBR	Unknown	32.43	1.043					76
F4	4.5 1.5 k	GAPDH_SYBR	Unknown	33.9	1.043					76
F5	4.5 4.5 k	GAPDH_SYBR	Unknown	Undetermined						67.1
F6	4.5 4.5 k	GAPDH_SYBR	Unknown	32.27						76