Document Name: A2 LaR odp sk1 24112010.sds 										
Plate Type: Absolute Quantification										
User: 7500RTPCR										
										
Document Information										
										
Operator: zdenek										
Run Date: Wednesday	 November 24	 2010 19:56:00								
Last Modified: Thursday	 November 25	 2010 06:47:36								
Instrument Type: Applied Biosystems 7500 Real-Time PCR System										
										
Comments:										
SDS v1.3.1										
										
Thermal Cycler Profile										
Stage	Repetitions	Temperature	Time	Ramp Rate	Auto Increment					
1	1	50.0 ｰC	2:00	100						
2	1	95.0 ｰC	10:00	100						
3	40	95.0 ｰC	0:15	100						
		60.0 ｰC	1:00	100						
Standard 7500 Mode										
Data Collection :  Stage 3 Step 1										
PCR Volume: 25 ｵL										
										
Well	Sample Name	Detector	Task	Ct	StdDev Ct	Qty	Mean Qty	StdDev Qty	Filtered	Tm
A1	HL60 bez EtOH	GAPDH	Unknown	22.17	3.042					
		cycD1	Unknown	26.72	0.425					
A2	HL60 bez EtOH	GAPDH	Unknown	20.9	3.042					
		cycD1	Unknown	26.16	0.425					
A3	HL60 bez EtOH	GAPDH	Unknown	26.69	3.042					
		cycD1	Unknown	27	0.425					
A4	NoRNA	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A5	NoRT	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
B1	HL60 ATRA bez EtOH	GAPDH	Unknown	21	0.253					
		cycD1	Unknown	26.14	0.15					
B2	HL60 ATRA bez EtOH	GAPDH	Unknown	20.54	0.253					
		cycD1	Unknown	25.86	0.15					
B3	HL60 ATRA bez EtOH	GAPDH	Unknown	20.96	0.253					
		cycD1	Unknown	26.1	0.15					
B4	NoRNA	GAPDH	Unknown	Undetermined					Noisy BL	
		cycD1	Unknown	Undetermined						
B5	NoRT	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
C1	"HL60 0,5 ul"	GAPDH	Unknown	22.66	0.935					
		cycD1	Unknown	26.45	0.417					
C2	"HL60 0,5 ul"	GAPDH	Unknown	21.17	0.935					
		cycD1	Unknown	25.81	0.417					
C3	"HL60 0,5 ul"	GAPDH	Unknown	20.93	0.935					
		cycD1	Unknown	25.66	0.417					
D1	HL60 1 ul	GAPDH	Unknown	22.23	2.681					
		cycD1	Unknown	26.42	3.648					
D2	HL60 1 ul	GAPDH	Unknown	26.02	2.681					
		cycD1	Unknown	31.37	3.648					
D3	HL60 1 ul	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	33.54	3.648