Document Name: A2 skupina 1 odp 23112010.sds 										
Plate Type: Absolute Quantification										
User: 7500RTPCR										
										
Document Information										
										
Operator: 7500RTPCR										
Run Date: Wednesday	 November 24	 2010 07:43:32								
Last Modified: Wednesday	 November 24	 2010 10:28:29								
Instrument Type: Applied Biosystems 7500 Real-Time PCR System										
										
Comments:										
SDS v1.3.1										
										
Thermal Cycler Profile										
Stage	Repetitions	Temperature	Time	Ramp Rate	Auto Increment					
1	1	50.0 °C	2:00	100						
2	1	95.0 °C	10:00	100						
3	40	95.0 °C	0:15	100						
		60.0 °C	1:00	100						
Standard 7500 Mode										
Data Collection :  Stage 3 Step 1										
PCR Volume: 25 µL										
										
Well	Sample Name	Detector	Task	Ct	StdDev Ct	Qty	Mean Qty	StdDev Qty	Filtered	Tm
D1	25 ng	cycD1	Unknown	26.8	0.099					
		GAPDH-human	Unknown	21.5	0.096					
D2	25 ng	cycD1	Unknown	27	0.099					
		GAPDH-human	Unknown	21.66	0.096					
D3	25 ng	cycD1	Unknown	26.89	0.099					
		GAPDH-human	Unknown	21.66	0.096					
D5	2.5 ng	cycD1	Unknown	30.9	0.42					
		GAPDH-human	Unknown	25.35	0.361					
D6	2.5 ng	cycD1	Unknown	31	0.42					
		GAPDH-human	Unknown	25.72	0.361					
D7	2.5 ng	cycD1	Unknown	30.23	0.42					
		GAPDH-human	Unknown	25	0.361					
D9	250 pg	cycD1	Unknown	Undetermined						
		GAPDH-human	Unknown	Undetermined						
D10	250 pg	cycD1	Unknown	34.01	0.117					
		GAPDH-human	Unknown	28.87	0.001					
D11	250 pg	cycD1	Unknown	34.18	0.117					
		GAPDH-human	Unknown	28.88	0.001					
E1	25 pg	cycD1	Unknown	Undetermined						
		GAPDH-human	Unknown	Undetermined						
E2	25 pg	cycD1	Unknown	37.56	0.294					
		GAPDH-human	Unknown	33.14	0.142					
E3	25 pg	cycD1	Unknown	37.97	0.294					
		GAPDH-human	Unknown	32.93	0.142					
E5	2.5pg	cycD1	Unknown	Undetermined						
		GAPDH-human	Unknown	36.28	0.453					
E6	2.5pg	cycD1	Unknown	38.98						
		GAPDH-human	Unknown	35.98	0.453					
E7	2.5pg	cycD1	Unknown	Undetermined						
		GAPDH-human	Unknown	35.39	0.453					
E9	0.25pg	cycD1	Unknown	Undetermined						
		GAPDH-human	Unknown	Undetermined						
E10	0.25pg	cycD1	Unknown	Undetermined						
		GAPDH-human	Unknown	39.38	0.347					
E11	0.25pg	cycD1	Unknown	Undetermined						
		GAPDH-human	Unknown	39.87	0.347					
F1	NoRT	cycD1	Unknown	Undetermined						
		GAPDH-human	Unknown	Undetermined						
F2	NoRT	cycD1	Unknown	Undetermined						
		GAPDH-human	Unknown	Undetermined						
F3	NoRT	cycD1	Unknown	Undetermined						
		GAPDH-human	Unknown	Undetermined						
F5	H2O	cycD1	Unknown	Undetermined						
		GAPDH-human	Unknown	Undetermined					Noisy BL	
F6	H2O	cycD1	Unknown	Undetermined						
		GAPDH-human	Unknown	Undetermined						
F7	H2O	cycD1	Unknown	Undetermined						
		GAPDH-human	Unknown	38.73