Document Name: EJ KM	 skupina 3-odp	 23.11.2010.sds 								
Plate Type: Absolute Quantification										
User: 7500RTPCR										
										
Document Information										
										
Operator: 7500RTPCR										
Run Date: Tuesday	 November 23	 2010 17:20:44								
Last Modified: Wednesday	 November 24	 2010 07:21:55								
Instrument Type: Applied Biosystems 7500 Real-Time PCR System										
										
Comments:										
SDS v1.3.1										
										
Thermal Cycler Profile										
Stage	Repetitions	Temperature	Time	Ramp Rate	Auto Increment					
1	1	50.0 °C	2:00	100						
2	1	95.0 °C	10:00	100						
3	40	95.0 °C	0:15	100						
		60.0 °C	1:00	100						
Standard 7500 Mode										
Data Collection :  Stage 3 Step 1										
PCR Volume: 25 µL										
										
Well	Sample Name	Detector	Task	Ct	StdDev Ct	Qty	Mean Qty	StdDev Qty	Filtered	Tm
A1	25 ng	GAPDH-human	Unknown	20.63	0.24					
		cycD1	Unknown	25.1	0.153					
A2	2.5 ng	GAPDH-human	Unknown	24.16	0.091					
		cycD1	Unknown	28.4	0.177					
A3	0.25 ng	GAPDH-human	Unknown	27.89	0.077					
		cycD1	Unknown	31.9	0.198					
A4	25 pg	GAPDH-human	Unknown	31.37	0.072					
		cycD1	Unknown	34.95	0.297					
A5	2.5 pg	GAPDH-human	Unknown	34.82	0.256					
		cycD1	Unknown	39.61	1.164					
A6	0.25 pg	GAPDH-human	Unknown	30.17	0.179					
		cycD1	Unknown	34.34	0.344					
A7	NoRT	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A8	NoRNA	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
B1	25 ng	GAPDH-human	Unknown	21	0.24					
		cycD1	Unknown	25.01	0.153					
B2	2.5 ng	GAPDH-human	Unknown	24.16	0.091					
		cycD1	Unknown	28.08	0.177					
B3	0.25 ng	GAPDH-human	Unknown	28.02	0.077					
		cycD1	Unknown	32.17	0.198					
B4	25 pg	GAPDH-human	Unknown	31.32	0.072					
		cycD1	Unknown	35.49	0.297					
B5	2.5 pg	GAPDH-human	Unknown	34.45	0.256					
		cycD1	Unknown	37.96	1.164					
B6	0.25 pg	GAPDH-human	Unknown	30.52	0.179					
		cycD1	Unknown	34.4	0.344					
B7	NoRT	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
B8	NoRNA	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
C1	25 ng	GAPDH-human	Unknown	21.08	0.24					
		cycD1	Unknown	24.8	0.153					
C2	2.5 ng	GAPDH-human	Unknown	24.32	0.091					
		cycD1	Unknown	28.1	0.177					
C3	0.25 ng	GAPDH-human	Unknown	28.03	0.077					
		cycD1	Unknown	31.78	0.198					
C4	25 pg	GAPDH-human	Unknown	31.46	0.072					
		cycD1	Unknown	35	0.297					
C5	2.5 pg	GAPDH-human	Unknown	34.94	0.256					
		cycD1	Unknown	Undetermined						
C6	0.25 pg	GAPDH-human	Unknown	30.42	0.179					
		cycD1	Unknown	33.78	0.344					
C7	NoRT	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
C8	NoRNA	GAPDH-human	Unknown	Undetermined						
		cycD1	Unknown	Undetermined