Document Name: EJ KM skupina3-odpo 24.11.2010.sds 										
Plate Type: Absolute Quantification										
User: 7500RTPCR										
										
Document Information										
										
Operator: zdenek										
Run Date: Thursday	 November 25	 2010 08:43:15								
Last Modified: Thursday	 November 25	 2010 10:40:42								
Instrument Type: Applied Biosystems 7500 Real-Time PCR System										
										
Comments:										
SDS v1.3.1										
										
Thermal Cycler Profile										
Stage	Repetitions	Temperature	Time	Ramp Rate	Auto Increment					
1	1	50.0 °C	2:00	100						
2	1	95.0 °C	10:00	100						
3	40	95.0 °C	0:15	100						
		60.0 °C	1:00	100						
Standard 7500 Mode										
Data Collection :  Stage 3 Step 1										
PCR Volume: 25 µL										
										
Well	Sample Name	Detector	Task	Ct	StdDev Ct	Qty	Mean Qty	StdDev Qty	Filtered	Tm
A1	25 ng nonT	GAPDH	Unknown	20.55	0.324					
		cycD1	Unknown	25.82	0.045					
A2	25 ng T	GAPDH	Unknown	21.86	0.074					
		cycD1	Unknown	27.27	0.138					
A3	no RNA	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A4	no RT nonT	GAPDH	Unknown	Undetermined						
		cycD1	Unknown	Undetermined						
A5	"0,5 EtOH nonT"	GAPDH	Unknown	21.08	0.142					
		cycD1	Unknown	25.77	0.285					
A6	1 EtOH nonT	GAPDH	Unknown	20.66	0.314					
		cycD1	Unknown	25.12	0.107					
B1	25 ng nonT	GAPDH	Unknown	20.95	0.324					
		cycD1	Unknown	25.86	0.045					
B2	25 ng T	GAPDH	Unknown	22	0.074					
		cycD1	Unknown	27.25	0.138					
B5	"0,5 EtOH nonT"	GAPDH	Unknown	21.14	0.142					
		cycD1	Unknown	25.75	0.285					
B6	1 EtOH nonT	GAPDH	Unknown	21.09	0.314					
		cycD1	Unknown	25	0.107					
C1	25 ng nonT	GAPDH	Unknown	21.19	0.324					
		cycD1	Unknown	25.77	0.045					
C2	25 ng T	GAPDH	Unknown	21.97	0.074					
		cycD1	Unknown	27.02	0.138					
C5	"0,5 EtOH nonT"	GAPDH	Unknown	20.87	0.142					
		cycD1	Unknown	25.27	0.285					
C6	1 EtOH nonT	GAPDH	Unknown	21.27	0.314					
		cycD1	Unknown	25.21	0.107