Document Name: PF 2010 skupina 2. 24.11. 2010.sds 										
Plate Type: Absolute Quantification										
User: 7500RTPCR										
										
Document Information										
										
Operator: zdenek										
Run Date: Wednesday	 November 24	 2010 17:32:39								
Last Modified: Wednesday	 November 24	 2010 19:39:06								
Instrument Type: Applied Biosystems 7500 Real-Time PCR System										
										
Comments:										
SDS v1.3.1										
										
Thermal Cycler Profile										
Stage	Repetitions	Temperature	Time	Ramp Rate	Auto Increment					
1	1	50.0 °C	2:00	100						
2	1	95.0 °C	10:00	100						
3	40	95.0 °C	0:15	100						
		60.0 °C	1:00	100						
4 (Dissociation)	1	95.0 °C	0:15	Auto						
		60.0 °C	1:00	Auto						
		95.0 °C	0:15	Auto						
Standard 7500 Mode										
Data Collection :  Stage 3 Step 1										
PCR Volume: 25 µL										
										
Well	Sample Name	Detector	Task	Ct	StdDev Ct	Qty	Mean Qty	StdDev Qty	Filtered	Tm
A1	N	SYBRgreen	Unknown	16	0.15					79.8
A2	N	SYBRgreen	Unknown	16.13	0.15					79.8
A3	N	SYBRgreen	Unknown	16.3	0.15					79.8
A4	No RT	SYBRgreen	Unknown	23.48	1.178					79.8
A5	No RNA	SYBRgreen	Unknown	34.91						75.7
B1	TPA	SYBRgreen	Unknown	17.71	0.042					79.8
B2	TPA	SYBRgreen	Unknown	17.67	0.042					79.8
B3	TPA	SYBRgreen	Unknown	17.75	0.042					79.8
B4	No RT	SYBRgreen	Unknown	21.82	1.178					79.8
C1	TPA	cycD1	Unknown	33	0.266					79.5
		GAPDH	Unknown	25.96	0.235					63.4
C2	TPA	cycD1	Unknown	32.73	0.266					87.1
		GAPDH	Unknown	25.68	0.235					86.4
C3	TPA	cycD1	Unknown	32.47	0.266					85
		GAPDH	Unknown	25.49	0.235					63.4
C4	No RT	cycD1	Unknown	Undetermined						81.2
		GAPDH	Unknown	Undetermined						63.4
C5	No RNA	cycD1	Unknown	Undetermined						79.5
		GAPDH	Unknown	Undetermined						63.4
D1	N	cycD1	Unknown	31.81	0.264					79.5
		GAPDH	Unknown	23.58	0.323					63.4
D2	N	cycD1	Unknown	32.06	0.264					74.3
		GAPDH	Unknown	24.05	0.323					63.4
D3	N	cycD1	Unknown	31.53	0.264					78.5
		GAPDH	Unknown	23.44	0.323					63.4
D4	No RT	cycD1	Unknown	Undetermined						85.4
		GAPDH	Unknown	Undetermined						63.4