Document Name: JitkaIvica.sds Plate Type: Absolute Quantification User: 7500RTPCR Document Information Operator: 7500RTPCR Run Date: Tuesday November 30 2010 10:02:24 Last Modified: Wednesday December 01 2010 08:25:22 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.3.1 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate Auto Increment 1 1 50.0 °C 2:00 100 2 1 95.0 °C 10:00 100 3 40 95.0 °C 0:15 100 60.0 °C 1:00 100 Standard 7500 Mode Data Collection : Stage 3 Step 1 PCR Volume: 25 µL Well Sample Name Detector Task Ct StdDev Ct Qty Mean Qty StdDev Qty Filtered Tm A1 NTC GAPDH-human Unknown Undetermined YWHAZ Unknown Undetermined A2 NTC GAPDH-human Unknown Undetermined YWHAZ Unknown Undetermined A3 no RT GAPDH-human Unknown Undetermined YWHAZ Unknown Undetermined A4 no RT GAPDH-human Unknown Undetermined YWHAZ Unknown Undetermined A5 no RT GAPDH-human Unknown Undetermined YWHAZ Unknown Undetermined A6 Jitka -nrd GAPDH-human Unknown 21.07 0.033 YWHAZ Unknown 27.64 0.096 A7 Jitka -nrd GAPDH-human Unknown 21.03 0.033 YWHAZ Unknown 27.5 0.096 A8 Jitka -nrd GAPDH-human Unknown 21.1 0.033 YWHAZ Unknown 27.46 0.096 A9 J-10x GAPDH-human Unknown 25.11 0.339 YWHAZ Unknown 31.58 0.43 A10 J-10x GAPDH-human Unknown 25.11 0.339 YWHAZ Unknown 31.58 0.43 A11 J-10x GAPDH-human Unknown 25.69 0.339 YWHAZ Unknown 32.33 0.43 A12 j-100x YWHAZ Unknown 34.68 GAPDH-human Unknown 28.62 B1 J 100x GAPDH-human Unknown 28.55 0.001 YWHAZ Unknown 35.7 0.081 B2 J 100x GAPDH-human Unknown 28.56 0.001 YWHAZ Unknown 35.59 0.081 B3 J-1000x GAPDH-human Unknown 32 0.157 YWHAZ Unknown 39.22 0.025 B4 J-1000x GAPDH-human Unknown 32.22 0.157 YWHAZ Unknown 39.18 0.025 B5 J-1000x GAPDH-human Unknown 31.92 0.157 YWHAZ Unknown Undetermined B6 J-100 00x GAPDH-human Unknown 35.76 0.023 YWHAZ Unknown Undetermined B7 J-100 00x GAPDH-human Unknown 35.71 0.023 YWHAZ Unknown Undetermined B8 J-100 00x GAPDH-human Unknown 35.75 0.023 YWHAZ Unknown Undetermined B9 J-100 000x GAPDH-human Unknown Undetermined YWHAZ Unknown Undetermined B10 J-100 000x GAPDH-human Unknown 39.25 0.537 YWHAZ Unknown Undetermined B11 J-100 000x GAPDH-human Unknown 38.49 0.537 YWHAZ Unknown Undetermined C1 NTC GAPDH-human Unknown Undetermined YWHAZ Unknown Undetermined C2 NTC GAPDH-human Unknown Undetermined YWHAZ Unknown Undetermined C3 no RT GAPDH-human Unknown Undetermined YWHAZ Unknown Undetermined C4 no RT GAPDH-human Unknown Undetermined YWHAZ Unknown Undetermined C5 no RT GAPDH-human Unknown Undetermined YWHAZ Unknown Undetermined C6 Ivica-nrd GAPDH-human Unknown 18.92 0.091 YWHAZ Unknown 25.8 0.073 C7 Ivica-nrd GAPDH-human Unknown 19.03 0.091 YWHAZ Unknown 25.82 0.073 C8 Ivica-nrd GAPDH-human Unknown 19.1 0.091 YWHAZ Unknown 25.93 0.073 C9 I-10x GAPDH-human Unknown 22.46 0.042 YWHAZ Unknown 29.32 0.007 C10 I-10x GAPDH-human Unknown 22.54 0.042 YWHAZ Unknown 29.33 0.007 C11 I-10x GAPDH-human Unknown 22.48 0.042 YWHAZ Unknown 29.33 0.007 C12 I-100x GAPDH-human Unknown 25.95 0.138 YWHAZ Unknown 32.78 0.08 D1 I-100x GAPDH-human Unknown 26.07 0.138 YWHAZ Unknown 32.83 0.08 D2 I-100x GAPDH-human Unknown 26.23 0.138 YWHAZ Unknown 32.94 0.08 D3 I-1000x GAPDH-human Unknown 29.4 0.121 YWHAZ Unknown 35.57 0.222 D4 I-1000x GAPDH-human Unknown 29.24 0.121 YWHAZ Unknown 35.68 0.222 D5 I-1000x GAPDH-human Unknown 29.17 0.121 YWHAZ Unknown 36 0.222 D6 I-10 000x GAPDH-human Unknown 32.51 0.137 YWHAZ Unknown 37.47 D7 I-10 000x GAPDH-human Unknown 32.76 0.137 YWHAZ Unknown Undetermined D8 I-10 000x GAPDH-human Unknown 32.53 0.137 YWHAZ Unknown Undetermined D9 I-100 000x GAPDH-human Unknown 36.33 0.22 YWHAZ Unknown Undetermined D10 I-100 000x GAPDH-human Unknown 36.76 0.22 YWHAZ Unknown Undetermined D11 I-100 000x GAPDH-human Unknown 36.5 0.22 YWHAZ Unknown 39.51