Document Name: KristynaEva analyzed.sds Plate Type: Standard Curve User: Administrator Document Information Operator: Administrator Run Date: Tuesday November 30 2010 10:24:21 Last Modified: Wednesday December 01 2010 08:20:23 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.4 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate Auto Increment 1 1 50.0 °C 2:00 100 2 1 95.0 °C 10:00 100 3 40 95.0 °C 0:15 100 60.0 °C 1:00 100 Standard 7500 Mode Data Collection : Stage 3 Step 2 PCR Volume: 25 µL Well Sample Name Detector Task Ct StdDev Ct Qty Mean Qty StdDev Qty Filtered Tm User Defined #1 User Defined #2 User Defined #3 A1 ntc CCND1 Unknown Undetermined A1 ntc GAPDH_VIC Unknown Undetermined A2 ntc CCND1 Unknown Undetermined A2 ntc GAPDH_VIC Unknown Undetermined A3 NOrt CCND1 Unknown Undetermined A3 NOrt GAPDH_VIC Unknown Undetermined A4 NOrt CCND1 Unknown Undetermined A4 NOrt GAPDH_VIC Unknown Undetermined A5 NOrt CCND1 Unknown Undetermined A5 NOrt GAPDH_VIC Unknown Undetermined A6 Kr-und CCND1 Unknown Undetermined A6 Kr-und GAPDH_VIC Unknown 20.9008 0.162 A7 Kr-und CCND1 Unknown Undetermined A7 Kr-und GAPDH_VIC Unknown 20.809 0.162 A8 Kr-und CCND1 Unknown Undetermined A8 Kr-und GAPDH_VIC Unknown 20.5864 0.162 A9 Kr-10x CCND1 Unknown Undetermined A9 Kr-10x GAPDH_VIC Unknown 23.8967 0.0795 A10 Kr-10x CCND1 Unknown Undetermined A10 Kr-10x GAPDH_VIC Unknown 24.0414 0.0795 A11 Kr-10x CCND1 Unknown Undetermined A11 Kr-10x GAPDH_VIC Unknown 24.026 0.0795 B1 Kr-100x CCND1 Unknown Undetermined B1 Kr-100x GAPDH_VIC Unknown 27.0135 0.0264 B2 Kr-100x CCND1 Unknown Undetermined B2 Kr-100x GAPDH_VIC Unknown 26.9772 0.0264 B3 Kr-100x CCND1 Unknown Undetermined B3 Kr-100x GAPDH_VIC Unknown 26.9621 0.0264 B4 Kr-1000x CCND1 Unknown Undetermined B4 Kr-1000x GAPDH_VIC Unknown 30.1207 0.0409 B5 Kr-1000x CCND1 Unknown Undetermined B5 Kr-1000x GAPDH_VIC Unknown 30.1681 0.0409 B6 Kr-1000x CCND1 Unknown Undetermined B6 Kr-1000x GAPDH_VIC Unknown 30.0866 0.0409 B7 Kr10 000x CCND1 Unknown Undetermined B7 Kr10 000x GAPDH_VIC Unknown 33.2989 0.0974 B8 Kr10 000x CCND1 Unknown Undetermined B8 Kr10 000x GAPDH_VIC Unknown 33.49 0.0974 B9 Kr10 000x CCND1 Unknown Undetermined B9 Kr10 000x GAPDH_VIC Unknown 33.3615 0.0974 C1 Kr 100 000x CCND1 Unknown Undetermined C1 Kr 100 000x GAPDH_VIC Unknown 36.3872 1.04 C2 Kr 100 000x CCND1 Unknown Undetermined C2 Kr 100 000x GAPDH_VIC Unknown 38.0373 1.04 C3 Kr 100 000x CCND1 Unknown Undetermined C3 Kr 100 000x GAPDH_VIC Unknown 36.1123 1.04 E1 ntc CCND1 Unknown Undetermined E1 ntc GAPDH_VIC Unknown Undetermined E2 ntc CCND1 Unknown Undetermined E2 ntc GAPDH_VIC Unknown Undetermined E3 E-und CCND1 Unknown Undetermined E3 E-und GAPDH_VIC Unknown 16.843 0.176 E4 E-und CCND1 Unknown Undetermined E4 E-und GAPDH_VIC Unknown 16.8672 0.176 E5 E-und CCND1 Unknown Undetermined E5 E-und GAPDH_VIC Unknown 16.5501 0.176 E6 E-10x CCND1 Unknown Undetermined E6 E-10x GAPDH_VIC Unknown 20.6109 0.0183 E7 E-10x CCND1 Unknown Undetermined E7 E-10x GAPDH_VIC Unknown 20.6393 0.0183 E8 E-10x CCND1 Unknown Undetermined E8 E-10x GAPDH_VIC Unknown 20.6451 0.0183 E9 E-100x CCND1 Unknown Undetermined E9 E-100x GAPDH_VIC Unknown 24.2306 0.0601 E10 E-100x CCND1 Unknown Undetermined E10 E-100x GAPDH_VIC Unknown 24.2828 0.0601 E11 E-100x CCND1 Unknown Undetermined E11 E-100x GAPDH_VIC Unknown 24.3504 0.0601 F1 E-1000x CCND1 Unknown Undetermined F1 E-1000x GAPDH_VIC Unknown 27.861 0.0746 F2 E-1000x CCND1 Unknown Undetermined F2 E-1000x GAPDH_VIC Unknown 28 0.0746 F3 E-1000x CCND1 Unknown Undetermined F3 E-1000x GAPDH_VIC Unknown 27.9774 0.0746 F4 E 10 000x CCND1 Unknown Undetermined F4 E 10 000x GAPDH_VIC Unknown 31.3807 0.108 F5 E 10 000x CCND1 Unknown Undetermined F5 E 10 000x GAPDH_VIC Unknown 31.266 0.108 F6 E 10 000x CCND1 Unknown Undetermined F6 E 10 000x GAPDH_VIC Unknown 31.1655 0.108 F7 E 100 000x CCND1 Unknown Undetermined F7 E 100 000x GAPDH_VIC Unknown 35.0142 0.27 F8 E 100 000x CCND1 Unknown Undetermined F8 E 100 000x GAPDH_VIC Unknown 34.4965 0.27 F9 E 100 000x CCND1 Unknown Undetermined F9 E 100 000x GAPDH_VIC Unknown 34.89 0.27 F10 no RT CCND1 Unknown Undetermined F10 no RT GAPDH_VIC Unknown Undetermined F11 no RT CCND1 Unknown Undetermined F11 no RT GAPDH_VIC Unknown Undetermined F12 no RT CCND1 Unknown Undetermined F12 no RT GAPDH_VIC Unknown Undetermined