Document Name: Kristyna Eva ethanol.sds 													
Plate Type: Standard Curve													
User: sevcikova													
													
Document Information													
													
Operator: Administrator													
Run Date: Wednesday	 December 01	 2010 10:16:07											
Last Modified: Wednesday	 December 01	 2010 12:29:48											
Instrument Type: Applied Biosystems 7500 Real-Time PCR System													
													
Comments:													
SDS v1.4													
													
Thermal Cycler Profile													
Stage	Repetitions	Temperature	Time	Ramp Rate	Auto Increment								
1	1	50.0 °C	2:00	100									
2	1	95.0 °C	10:00	100									
3	40	95.0 °C	0:15	100									
		60.0 °C	1:00	100									
Standard 7500 Mode													
Data Collection :  Stage 3 Step 2													
PCR Volume: 25 µL													
													
Well	Sample Name	Detector	Task	Ct	StdDev Ct	Qty	Mean Qty	StdDev Qty	Filtered	Tm	User Defined #1	User Defined #2	User Defined #3
A1	ntc	MAF	Unknown	Undetermined									
A1	ntc	GAPDH_VIC	Unknown	Undetermined									
A2	ntc	MAF	Unknown	Undetermined									
A2	ntc	GAPDH_VIC	Unknown	Undetermined									
A3	noRT	MAF	Unknown	Undetermined									
A3	noRT	GAPDH_VIC	Unknown	Undetermined									
A4	noRT	MAF	Unknown	Undetermined									
A4	noRT	GAPDH_VIC	Unknown	Undetermined									
A5	noRT	MAF	Unknown	Undetermined									
A5	noRT	GAPDH_VIC	Unknown	Undetermined									
A6	ATRA 10x	MAF	Unknown	Undetermined									
A6	ATRA 10x	GAPDH_VIC	Unknown	23.8496	0.106								
A7	ATRA 10x	MAF	Unknown	Undetermined									
A7	ATRA 10x	GAPDH_VIC	Unknown	Undetermined	0.106								
A8	ATRA 10x	MAF	Unknown	Undetermined									
A8	ATRA 10x	GAPDH_VIC	Unknown	24	0.106								
A9	ATRA 10x etha	MAF	Unknown	Undetermined									
A9	ATRA 10x etha	GAPDH_VIC	Unknown	Undetermined									
A10	ATRA 10x etha	MAF	Unknown	Undetermined									
A10	ATRA 10x etha	GAPDH_VIC	Unknown	Undetermined									
A11	ATRA 10x etha	MAF	Unknown	Undetermined									
A11	ATRA 10x etha	GAPDH_VIC	Unknown	Undetermined									
C1	ntc	MAF	Unknown	Undetermined									
C1	ntc	GAPDH_VIC	Unknown	Undetermined									
C2	ntc	MAF	Unknown	Undetermined									
C2	ntc	GAPDH_VIC	Unknown	Undetermined									
C3	noRT	MAF	Unknown	Undetermined									
C3	noRT	GAPDH_VIC	Unknown	Undetermined									
C4	noRT	MAF	Unknown	Undetermined									
C4	noRT	GAPDH_VIC	Unknown	Undetermined									
C5	noRT	MAF	Unknown	Undetermined									
C5	noRT	GAPDH_VIC	Unknown	Undetermined									
C6	untr 10x	MAF	Unknown	Undetermined									
C6	untr 10x	GAPDH_VIC	Unknown	20.166	0.356								
C7	untr 10x	MAF	Unknown	Undetermined									
C7	untr 10x	GAPDH_VIC	Unknown	20.5878	0.356								
C8	untr 10x	MAF	Unknown	Undetermined									
C8	untr 10x	GAPDH_VIC	Unknown	20.8741	0.356								
C9	10x eth	MAF	Unknown	Undetermined									
C9	10x eth	GAPDH_VIC	Unknown	Undetermined									
C10	10x eth	MAF	Unknown	Undetermined									
C10	10x eth	GAPDH_VIC	Unknown	Undetermined									
C11	10x eth	MAF	Unknown	Undetermined									
C11	10x eth	GAPDH_VIC	Unknown	Undetermined