Document Name: JitkaIvica ethanol.sds 													
Plate Type: Standard Curve													
User: sevcikova													
													
Document Information													
													
Operator: 7500RTPCR													
Run Date: Wednesday	 December 01	 2010 09:56:28											
Last Modified: Wednesday	 December 01	 2010 12:28:04											
Instrument Type: Applied Biosystems 7500 Real-Time PCR System													
													
Comments:													
SDS v1.3.1													
													
Thermal Cycler Profile													
Stage	Repetitions	Temperature	Time	Ramp Rate	Auto Increment								
1	1	50.0 °C	2:00	100									
2	1	95.0 °C	10:00	100									
3	40	95.0 °C	0:15	100									
		60.0 °C	1:00	100									
Standard 7500 Mode													
Data Collection :  Stage 3 Step 2													
PCR Volume: 25 µL													
													
Well	Sample Name	Detector	Task	Ct	StdDev Ct	Qty	Mean Qty	StdDev Qty	Filtered	Tm	User Defined #1	User Defined #2	User Defined #3
B1	NTC	YWHAZ	Unknown	Undetermined									
B1	NTC	GAPDH-human	Unknown	Undetermined									
B2	NTC	YWHAZ	Unknown	Undetermined									
B2	NTC	GAPDH-human	Unknown	Undetermined									
B3	untreat	YWHAZ	Unknown	32.9666	0.343								
B3	untreat	GAPDH-human	Unknown	28.7042	0.219								
B4	untreat	YWHAZ	Unknown	32.3699	0.343								
B4	untreat	GAPDH-human	Unknown	28.319	0.219								
B5	untreat	YWHAZ	Unknown	32.3745	0.343								
B5	untreat	GAPDH-human	Unknown	28.3302	0.219								
B6	treat	YWHAZ	Unknown	31.9427	0.0441								
B6	treat	GAPDH-human	Unknown	27.1456	0.157								
B7	treat	YWHAZ	Unknown	31.9132	0.0441								
B7	treat	GAPDH-human	Unknown	27.3684	0.157								
B8	treat	YWHAZ	Unknown	32	0.0441								
B8	treat	GAPDH-human	Unknown	27.4474	0.157								
C1	ethanol NTC	YWHAZ	Unknown	Undetermined									
C1	ethanol NTC	GAPDH-human	Unknown	Undetermined									
C2	ethanol NTC	YWHAZ	Unknown	Undetermined									
C2	ethanol NTC	GAPDH-human	Unknown	Undetermined									
C3	untreat eth	YWHAZ	Unknown	Undetermined									
C3	untreat eth	GAPDH-human	Unknown	Undetermined									
C4	untreat eth	YWHAZ	Unknown	Undetermined									
C4	untreat eth	GAPDH-human	Unknown	Undetermined									
C5	untreat eth	YWHAZ	Unknown	Undetermined									
C5	untreat eth	GAPDH-human	Unknown	Undetermined									
C6	treat etha	YWHAZ	Unknown	Undetermined									
C6	treat etha	GAPDH-human	Unknown	Undetermined									
C7	treat etha	YWHAZ	Unknown	Undetermined									
C7	treat etha	GAPDH-human	Unknown	Undetermined									
C8	treat etha	YWHAZ	Unknown	Undetermined									
C8	treat etha	GAPDH-human	Unknown	Undetermined