Document Name: skupina 2 ethanol.sds Plate Type: Standard Curve User: Administrator Document Information Operator: Administrator Run Date: Wednesday December 01 2010 12:07:42 Last Modified: Thursday December 02 2010 13:18:10 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Exporting Mode: Based on Report Setting Comments: SDS v1.4 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate Auto Increment 1 1 50.0 ｰC 2:00 100 2 1 95.0 ｰC 10:00 100 3 40 95.0 ｰC 0:15 100 60.0 ｰC 1:00 100 4 (Dissociation) 1 95.0 ｰC 0:15 Auto 60.0 ｰC 1:00 Auto 95.0 ｰC 0:15 Auto 60.0 ｰC 0:15 Auto Standard 7500 Mode Data Collection : Stage 3 Step 2 PCR Volume: 25 ｵL Well Sample Name Detector Task Ct StdDev Ct Quantity Mean Qty StdDev Qty Filtered Tm User Defined #1 User Defined #2 User Defined #3 A1 GAPDH-SYBR Unknown 36.3759 -999.9 74.8 A2 GAPDH-SYBR Unknown 35.4183 -999.9 80 A3 GAPDH-SYBR Unknown Undetermined Noisy BL 68.6 A4 GAPDH-SYBR Unknown 35.8125 -999.9 75.4 A5 GAPDH-SYBR Unknown 36 -999.9 75.1 A6 GAPDH-SYBR Unknown 38.3905 -999.9 83.8 A7 GAPDH-SYBR Unknown Undetermined 65.8 A8 GAPDH-SYBR Unknown Undetermined 65.8 A9 GAPDH-SYBR Unknown 28.998 -999.9 86.6 A10 GAPDH-SYBR Unknown Undetermined A11 GAPDH-SYBR Unknown Undetermined Noisy BL 81 A12 GAPDH-SYBR Unknown 5.77597 -999.9 76.5 B1 GAPDH-SYBR Unknown 16.4254 -999.9 79.6 B2 GAPDH-SYBR Unknown 16.6497 -999.9 79.6 B3 GAPDH-SYBR Unknown 16.6498 -999.9 80 B4 GAPDH-SYBR Unknown 18.4402 -999.9 79.6 B5 GAPDH-SYBR Unknown 18.2981 -999.9 80 B6 GAPDH-SYBR Unknown 18.3298 -999.9 79.6 B7 GAPDH-SYBR Unknown Undetermined 65.8 B8 GAPDH-SYBR Unknown Undetermined 65.8 B9 GAPDH-SYBR Unknown Undetermined 65.8 B10 GAPDH-SYBR Unknown Undetermined B11 GAPDH-SYBR Unknown Undetermined Noisy BL 66.2 B12 GAPDH-SYBR Unknown Undetermined Noisy BL C1 GAPDH-SYBR Unknown 19.0178 -999.9 79.6 C2 GAPDH-SYBR Unknown 19.0676 -999.9 80 C3 GAPDH-SYBR Unknown 19.2235 -999.9 80 C4 GAPDH-SYBR Unknown 20.9151 -999.9 80 C5 GAPDH-SYBR Unknown 20.9293 -999.9 80 C6 GAPDH-SYBR Unknown 20.9971 -999.9 80 C7 GAPDH-SYBR Unknown Undetermined 65.8 C8 GAPDH-SYBR Unknown Undetermined 65.8 C9 GAPDH-SYBR Unknown Undetermined 65.8 C10 GAPDH-SYBR Unknown Undetermined C11 GAPDH-SYBR Unknown Undetermined Noisy BL 68.6 C12 GAPDH-SYBR Unknown Undetermined Noisy BL 74.1 D1 GAPDH-SYBR Unknown 22.1793 -999.9 80 D2 GAPDH-SYBR Unknown 22.2481 -999.9 80 D3 GAPDH-SYBR Unknown 22.2132 -999.9 80.3 D4 GAPDH-SYBR Unknown 24.2201 -999.9 80 D5 GAPDH-SYBR Unknown 24.1798 -999.9 80 D6 GAPDH-SYBR Unknown 24.3664 -999.9 80 D7 GAPDH-SYBR Unknown Undetermined 66.5 D8 GAPDH-SYBR Unknown Undetermined 65.8 D9 GAPDH-SYBR Unknown Undetermined 65.8 D10 GAPDH-SYBR Unknown Undetermined Noisy BL 65.8 D11 GAPDH-SYBR Unknown Undetermined Noisy BL 67.9 D12 GAPDH-SYBR Unknown Undetermined 83.4 E1 GAPDH-SYBR Unknown 25.4109 -999.9 80 E2 GAPDH-SYBR Unknown 25.4521 -999.9 80 E3 GAPDH-SYBR Unknown 25.413 -999.9 80 E4 GAPDH-SYBR Unknown 27.298 -999.9 80 E5 GAPDH-SYBR Unknown 27.3038 -999.9 80 E6 GAPDH-SYBR Unknown 27.5431 -999.9 80 E7 GAPDH-SYBR Unknown Undetermined 65.8 E8 GAPDH-SYBR Unknown Undetermined 66.2 E9 GAPDH-SYBR Unknown Undetermined 67.2 E10 GAPDH-SYBR Unknown Undetermined 77.9 E11 GAPDH-SYBR Unknown 13.2967 -999.9 79.6 E12 GAPDH-SYBR Unknown Undetermined Noisy BL 65.8 F1 GAPDH-SYBR Unknown 29.0437 -999.9 79.6 F2 GAPDH-SYBR Unknown 28.6229 -999.9 80 F3 GAPDH-SYBR Unknown 28.5482 -999.9 80 F4 GAPDH-SYBR Unknown 31.25 -999.9 80 F5 GAPDH-SYBR Unknown 30.9286 -999.9 79.6 F6 GAPDH-SYBR Unknown 30.926 -999.9 80 F7 GAPDH-SYBR Unknown Undetermined 65.8 F8 GAPDH-SYBR Unknown Undetermined 65.8 F9 GAPDH-SYBR Unknown Undetermined 65.8 F10 GAPDH-SYBR Unknown Undetermined 73.4 F11 GAPDH-SYBR Unknown Undetermined 67.9 F12 GAPDH-SYBR Unknown Undetermined 66.2 G1 GAPDH-SYBR Unknown 32.0634 -999.9 79.3 G2 GAPDH-SYBR Unknown 32.2249 -999.9 79.3 G3 GAPDH-SYBR Unknown 31.8977 -999.9 79.6 G4 GAPDH-SYBR Unknown 33.9868 -999.9 80 G5 GAPDH-SYBR Unknown 35.3806 -999.9 75.8 G6 GAPDH-SYBR Unknown 35.424 -999.9 79.6 G7 GAPDH-SYBR Unknown Undetermined 65.8 G8 GAPDH-SYBR Unknown Undetermined 65.8 G9 GAPDH-SYBR Unknown Undetermined 65.8 G10 GAPDH-SYBR Unknown 21.5922 -999.9 66.5 G11 GAPDH-SYBR Unknown Undetermined 73.4 G12 GAPDH-SYBR Unknown 6.99996 -999.9 66.9 H1 GAPDH-SYBR Unknown Undetermined Noisy BL 81.7 H2 GAPDH-SYBR Unknown Undetermined Noisy BL 74.8 H3 GAPDH-SYBR Unknown 14.8386 -999.9 69.9 H4 GAPDH-SYBR Unknown Undetermined 87.6 H5 GAPDH-SYBR Unknown Undetermined 71.7 H6 GAPDH-SYBR Unknown Undetermined 75.1 H7 GAPDH-SYBR Unknown Undetermined 71.7 H8 GAPDH-SYBR Unknown Undetermined 76.8 H9 GAPDH-SYBR Unknown Undetermined 82.7 H10 GAPDH-SYBR Unknown Undetermined 72 H11 GAPDH-SYBR Unknown Undetermined Noisy BL 83.4 H12 GAPDH-SYBR Unknown 3.00182 -999.9 78.2