Document Name: A549-izolace_U-2(M MI DM DMI 0E) 2.3.2010.sds Plate Type: Absolute Quantification User: 7500RTPCR Document Information Operator: 7500RTPCR Run Date: Tuesday March 02 2010 14:57:18 Last Modified: Tuesday March 02 2010 17:19:04 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.3.1 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate Auto Increment 1 1 50.0 ｰC 2:00 100 2 1 95.0 ｰC 10:00 100 3 50 95.0 ｰC 0:15 100 60.0 ｰC 1:00 100 Standard 7500 Mode Data Collection : Stage 3 Step 1 PCR Volume: 25 ｵL Well Sample Name Detector Task Ct StdDev Ct Qty Mean Qty StdDev Qty Filtered Tm A1 0-A GAPDH-human Unknown 15.45 0.116 SFTPA2 Unknown 18.23 0.32 A2 0-A GAPDH-human Unknown 15.47 0.116 SFTPA2 Unknown 18.44 0.32 A3 0-A GAPDH-human Unknown 15.34 0.116 SFTPA2 Unknown 18.32 0.101 A4 0-B GAPDH-human Unknown 15.05 0.116 SFTPA2 Unknown 18.01 0.101 A5 0-B GAPDH-human Unknown 14.9 0.116 SFTPA2 Unknown 18.46 0.055 A6 0-B GAPDH-human Unknown 14.95 0.116 SFTPA2 Unknown 18.54 0.055 A7 0-C GAPDH-human Unknown 15.8 0.1 SFTPA2 Unknown 19.01 0.223 A8 0-C GAPDH-human Unknown 15.55 0.1 SFTPA2 Unknown 18.51 0.223 A9 0-C GAPDH-human Unknown 15.23 0.1 SFTPA2 Unknown 18.66 0.038 A10 "0,25-A" GAPDH-human Unknown 14.81 0.1 SFTPA2 Unknown 18.23 0.038 A11 "0,25-A" GAPDH-human Unknown 14.99 0.1 SFTPA2 Unknown 18.65 0.346 A12 "0,25-A" GAPDH-human Unknown 15.62 0.1 SFTPA2 Unknown 18.99 0.346 B1 "0,25-B" GAPDH-human Unknown 16.01 0.183 SFTPA2 Unknown 18.46 0.284 B2 "0,25-B" GAPDH-human Unknown 15.16 0.183 SFTPA2 Unknown 18.21 0.284 B3 "0,25-B" GAPDH-human Unknown 15.84 0.183 SFTPA2 Unknown 18.68 0.053 B4 "0,25-C" GAPDH-human Unknown 15.81 0.183 SFTPA2 Unknown 18.97 0.053 B5 "0,25-C" GAPDH-human Unknown 15.72 0.183 SFTPA2 Unknown 18.99 0.019 B6 "0,25-C" GAPDH-human Unknown 16.03 0.183 SFTPA2 Unknown 18.64 0.019 B7 "2,5-A" GAPDH-human Unknown 16 0.183 SFTPA2 Unknown 19.01 0.284 B8 "2,5-A" GAPDH-human Unknown 15.46 0.183 SFTPA2 Unknown 18.54 0.284 B9 "2,5-A" GAPDH-human Unknown 15.65 0.183 SFTPA2 Unknown 18.69 0.053 B10 "2,5-B" GAPDH-human Unknown 16.09 0.183 SFTPA2 Unknown 18.57 0.053 B11 "2,5-B" GAPDH-human Unknown 15.74 0.183 SFTPA2 Unknown 18.83 0.019 B12 "2,5-B" GAPDH-human Unknown 15.53 0.183 SFTPA2 Unknown 18.91 0.019 C1 "2,5-C" GAPDH-human Unknown 15.35 0.116 SFTPA2 Unknown 18.77 0.32 C2 "2,5-C" GAPDH-human Unknown 15.21 0.116 SFTPA2 Unknown 19.05 0.32 C3 "2,5-C" GAPDH-human Unknown 14.98 0.116 SFTPA2 Unknown 19.11 0.101 C4 25-A GAPDH-human Unknown 15.32 0.116 SFTPA2 Unknown 19.03 0.101 C5 25-A GAPDH-human Unknown 14.84 0.116 SFTPA2 Unknown 18.44 0.055 C6 25-A GAPDH-human Unknown 15.12 0.116 SFTPA2 Unknown 18.69 0.055 C7 25-B GAPDH-human Unknown 15.33 0.1 SFTPA2 Unknown 18.77 0.223 C8 25-B GAPDH-human Unknown 15.76 0.1 SFTPA2 Unknown 18.98 0.223 C9 25-B GAPDH-human Unknown 15.87 0.1 SFTPA2 Unknown 18.56 0.038 C10 25-C GAPDH-human Unknown 15.61 0.1 SFTPA2 Unknown 18.67 0.038 C11 25-C GAPDH-human Unknown 15.62 0.1 SFTPA2 Unknown 19.01 0.346 C12 25-C GAPDH-human Unknown 14.91 0.1 SFTPA2 Unknown 18.33 0.346 D1 250-A GAPDH-human Unknown 16.32 0.183 SFTPA2 Unknown 19.55 0.284 D2 250-A GAPDH-human Unknown 15.24 0.183 SFTPA2 Unknown 18.58 0.284 D3 250-A GAPDH-human Unknown 15.68 0.183 SFTPA2 Unknown 19.02 0.053 D4 250-B GAPDH-human Unknown 15.54 0.183 SFTPA2 Unknown 19.11 0.053 D5 250-B GAPDH-human Unknown 15.15 0.183 SFTPA2 Unknown 18.76 0.019 D6 250-B GAPDH-human Unknown 16.07 0.183 SFTPA2 Unknown 19.5 0.019 D7 250-C GAPDH-human Unknown 15.51 0.116 SFTPA2 Unknown 18.88 0.32 D8 250-C GAPDH-human Unknown 15.62 0.116 SFTPA2 Unknown 18.76 0.32 D9 250-C GAPDH-human Unknown 15.32 0.116 SFTPA2 Unknown 18.71 0.101 D10 2500-A GAPDH-human Unknown 15.22 0.116 SFTPA2 Unknown 18.51 0.101 D11 2500-A GAPDH-human Unknown 14.89 0.116 SFTPA2 Unknown 18.31 0.055 D12 2500-A GAPDH-human Unknown 15.35 0.116 SFTPA2 Unknown 18.24 0.055 E1 2500-B GAPDH-human Unknown 15.27 0.183 SFTPA2 Unknown 18.61 0.284 E2 2500-B GAPDH-human Unknown 15.67 0.183 SFTPA2 Unknown 18.37 0.284 E3 2500-B GAPDH-human Unknown 15.22 0.183 SFTPA2 Unknown 18.59 0.053 E4 2500-C GAPDH-human Unknown 15.01 0.183 SFTPA2 Unknown 18.22 0.053 E5 2500-C GAPDH-human Unknown 14.79 0.183 SFTPA2 Unknown 18.71 0.019 E6 2500-C GAPDH-human Unknown 15.44 0.183 SFTPA2 Unknown 18.38 0.019 E7 No RT GAPDH-human Unknown Undetected SFTPA2 Unknown Undetected E8 No RT GAPDH-human Unknown Undetected SFTPA2 Unknown Undetected E9 No RNA GAPDH-human Unknown Undetected SFTPA2 Unknown Undetected E10 No RNA GAPDH-human Unknown Undetected SFTPA2 Unknown Undetected E11 E12 F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12 G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 H1 H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12