Document Name: A549-izolace_U-2(M MI DM DMI 0E) 2.3.2010.sds Plate Type: Absolute Quantification User: 7500RTPCR Document Information Operator: 7500RTPCR Run Date: Tuesday March 02 2010 14:57:18 Last Modified: Tuesday March 02 2010 17:19:04 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.3.1 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate Auto Increment 1 1 50.0 ｰC 2:00 100 2 1 95.0 ｰC 10:00 100 3 50 95.0 ｰC 0:15 100 60.0 ｰC 1:00 100 Standard 7500 Mode Data Collection : Stage 3 Step 1 PCR Volume: 25 ｵL Well Sample Name Detector Task Ct StdDev Ct Qty Mean Qty StdDev Qty Filtered Tm A1 100 ng GAPDH-human Unknown 15.45 0.116 SFTPA1 Unknown 22.51 0.32 A2 100 ng GAPDH-human Unknown 15.47 0.116 SFTPA1 Unknown 22.45 0.32 A3 100 ng GAPDH-human Unknown 15.34 0.116 SFTPA1 Unknown 22.32 0.101 A4 10 ng GAPDH-human Unknown 19.11 0.116 SFTPA1 Unknown 22.11 0.101 A5 10 ng GAPDH-human Unknown 18.7 0.116 SFTPA1 Unknown 24.19 0.055 A6 10 ng GAPDH-human Unknown 18.72 0.116 SFTPA1 Unknown 23.99 0.055 A7 1 ng GAPDH-human Unknown 22.88 0.1 SFTPA1 Unknown 28.01 0.223 A8 1 ng GAPDH-human Unknown 22.74 0.1 SFTPA1 Unknown 28.22 0.223 A9 1 ng GAPDH-human Unknown 22.69 0.1 SFTPA1 Unknown 27.98 0.038 A10 100 pg GAPDH-human Unknown 26.03 0.1 SFTPA1 Unknown 32.05 0.038 A11 100 pg GAPDH-human Unknown 25.94 0.1 SFTPA1 Unknown 32.07 0.346 A12 100 pg GAPDH-human Unknown 25.81 0.1 SFTPA1 Unknown 32.1 0.346 B1 10 pg GAPDH-human Unknown 28.11 0.183 SFTPA1 Unknown 35.21 0.284 B2 10 pg GAPDH-human Unknown 28.46 0.183 SFTPA1 Unknown 35.11 0.284 B3 10 pg GAPDH-human Unknown 28.49 0.183 SFTPA1 Unknown 34.87 0.053 B4 1 pg GAPDH-human Unknown 32.02 0.183 SFTPA1 Unknown 39.07 0.053 B5 1 pg GAPDH-human Unknown 31.87 0.183 SFTPA1 Unknown 38.7 0.019 B6 1 pg GAPDH-human Unknown 31.73 0.183 SFTPA1 Unknown 38.77 0.019 B7 No RT GAPDH-human Unknown Undetermined SFTPA1 Unknown Undetermined B8 NTC GAPDH-human Unknown Undetermined SFTPA1 Unknown Undetermined B9 B10 B11 B12 C1 100 ng GAPDH-human Unknown 15.35 0.116 SFTPA2 Unknown 23.51 0.32 C2 100 ng GAPDH-human Unknown 15.21 0.116 SFTPA2 Unknown 23.45 0.32 C3 100 ng GAPDH-human Unknown 14.98 0.116 SFTPA2 Unknown 23.89 0.101 C4 10 ng GAPDH-human Unknown 19.02 0.116 SFTPA2 Unknown 26.01 0.101 C5 10 ng GAPDH-human Unknown 18.7 0.116 SFTPA2 Unknown 25.98 0.055 C6 10 ng GAPDH-human Unknown 18.64 0.116 SFTPA2 Unknown 26.15 0.055 C7 1 ng GAPDH-human Unknown 22.52 0.1 SFTPA2 Unknown 29.5 0.223 C8 1 ng GAPDH-human Unknown 22.41 0.1 SFTPA2 Unknown 29.86 0.223 C9 1 ng GAPDH-human Unknown 22.32 0.1 SFTPA2 Unknown 29.77 0.038 C10 100 pg GAPDH-human Unknown 25.81 0.1 SFTPA2 Unknown 33.69 0.038 C11 100 pg GAPDH-human Unknown 25.74 0.1 SFTPA2 Unknown 33.9 0.346 C12 100 pg GAPDH-human Unknown 25.69 0.1 SFTPA2 Unknown 33.89 0.346 D1 10 pg GAPDH-human Unknown 27.55 0.183 SFTPA2 Unknown 37.01 0.284 D2 10 pg GAPDH-human Unknown 27.62 0.183 SFTPA2 Unknown 37.2 0.284 D3 10 pg GAPDH-human Unknown 27.42 0.183 SFTPA2 Unknown 37.33 0.053 D4 1 pg GAPDH-human Unknown 31.01 0.183 SFTPA2 Unknown 39.01 0.053 D5 1 pg GAPDH-human Unknown 31.2 0.183 SFTPA2 Unknown 39.92 0.019 D6 1 pg GAPDH-human Unknown 30.89 0.183 SFTPA2 Unknown Undetermined D7 No RT GAPDH-human Unknown Undetermined SFTPA2 Unknown Undetermined D8 NTC GAPDH-human Unknown Undetermined SFTPA2 Unknown Undetermined D9 D10 D11 D12 E1 100 ng GAPDH-human Unknown 15.51 0.116 SFTPB Unknown 26.01 0.32 E2 100 ng GAPDH-human Unknown 15.62 0.116 SFTPB Unknown 26.1 0.32 E3 100 ng GAPDH-human Unknown 15.32 0.116 SFTPB Unknown 26.36 0.101 E4 10 ng GAPDH-human Unknown 19.21 0.116 SFTPB Unknown 28.94 0.101 E5 10 ng GAPDH-human Unknown 18.82 0.116 SFTPB Unknown 28.86 0.055 E6 10 ng GAPDH-human Unknown 18.76 0.116 SFTPB Unknown 28.74 0.055 E7 1 ng GAPDH-human Unknown 22.99 0.1 SFTPB Unknown 31.99 0.223 E8 1 ng GAPDH-human Unknown 22.81 0.1 SFTPB Unknown 32.54 0.223 E9 1 ng GAPDH-human Unknown 22.71 0.1 SFTPB Unknown 32.45 0.038 E10 100 pg GAPDH-human Unknown 26.03 0.1 SFTPB Unknown 35.6 0.038 E11 100 pg GAPDH-human Unknown 25.94 0.1 SFTPB Unknown 35.84 0.346 E12 100 pg GAPDH-human Unknown 25.89 0.1 SFTPB Unknown 35.23 0.346 F1 10 pg GAPDH-human Unknown 28.13 0.183 SFTPB Unknown 38.96 0.284 F2 10 pg GAPDH-human Unknown 29.51 0.183 SFTPB Unknown 38.21 0.284 F3 10 pg GAPDH-human Unknown 28.6 0.183 SFTPB Unknown 38.53 0.053 F4 1 pg GAPDH-human Unknown 32.12 0.183 SFTPB Unknown Undetermined 0.053 F5 1 pg GAPDH-human Unknown 32.05 0.183 SFTPB Unknown Undetermined 0.019 F6 1 pg GAPDH-human Unknown 31.97 0.183 SFTPB Unknown Undetermined 0.019 F7 No RT GAPDH-human Unknown Undetermined SFTPB Unknown Undetermined F8 NTC GAPDH-human Unknown Undetermined SFTPB Unknown Undetermined F9 F10 F11 F12 G1 100 ng GAPDH-human Unknown 14.98 0.116 SFTPC Unknown 30.65 0.32 G2 100 ng GAPDH-human Unknown 15.55 0.116 SFTPC Unknown 30.79 0.32 G3 100 ng GAPDH-human Unknown 15.23 0.116 SFTPC Unknown 30.84 0.101 G4 10 ng GAPDH-human Unknown 19.21 0.116 SFTPC Unknown 33.26 0.101 G5 10 ng GAPDH-human Unknown 18.82 0.116 SFTPC Unknown 33.31 0.055 G6 10 ng GAPDH-human Unknown 18.76 0.116 SFTPC Unknown 33.51 0.055 G7 1 ng GAPDH-human Unknown 22.99 0.1 SFTPC Unknown 37.12 0.223 G8 1 ng GAPDH-human Unknown 22.81 0.1 SFTPC Unknown 36.79 0.223 G9 1 ng GAPDH-human Unknown 22.71 0.1 SFTPC Unknown 36.93 0.038 G10 100 pg GAPDH-human Unknown 26.03 0.1 SFTPC Unknown Undetermined G11 100 pg GAPDH-human Unknown 25.94 0.1 SFTPC Unknown 39.76 0.346 G12 100 pg GAPDH-human Unknown 25.89 0.1 SFTPC Unknown Undetermined H1 10 pg GAPDH-human Unknown 28.13 0.183 SFTPC Unknown Undetermined H2 10 pg GAPDH-human Unknown 29.51 0.183 SFTPC Unknown Undetermined H3 10 pg GAPDH-human Unknown 28.6 0.183 SFTPC Unknown Undetermined H4 1 pg GAPDH-human Unknown 31.81 0.183 SFTPC Unknown Undetermined 0.053 H5 1 pg GAPDH-human Unknown 32.01 0.183 SFTPC Unknown Undetermined 0.019 H6 1 pg GAPDH-human Unknown 31.74 0.183 SFTPC Unknown Undetermined 0.019 H7 No RT GAPDH-human Unknown Undetermined SFTPC Unknown Undetermined H8 NTC GAPDH-human Unknown Undetermined SFTPC Unknown Undetermined H9 H10 H11 H12