Document Name: A549-izolace_U-2(M MI DM DMI 0E) 2.3.2010.sds Plate Type: Absolute Quantification User: 7500RTPCR Document Information Operator: 7500RTPCR Run Date: Tuesday March 02 2010 14:57:18 Last Modified: Tuesday March 02 2010 17:19:04 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.3.1 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate Auto Increment 1 1 50.0 ｰC 2:00 100 2 1 95.0 ｰC 10:00 100 3 50 95.0 ｰC 0:15 100 60.0 ｰC 1:00 100 Standard 7500 Mode Data Collection : Stage 3 Step 1 PCR Volume: 25 ｵL Well Sample Name Detector Task Ct StdDev Ct Qty Mean Qty StdDev Qty Filtered Tm A1 100 ng GAPDH-human Unknown 14.98 0.116 SFTPD Unknown 24.11 0.32 A2 100 ng GAPDH-human Unknown 15.21 0.116 SFTPD Unknown 24.23 0.32 A3 100 ng GAPDH-human Unknown 15.13 0.116 SFTPD Unknown 24.65 0.101 A4 10 ng GAPDH-human Unknown 19.11 0.116 SFTPD Unknown 27.97 0.101 A5 10 ng GAPDH-human Unknown 18.7 0.116 SFTPD Unknown 27.99 0.055 A6 10 ng GAPDH-human Unknown 18.72 0.116 SFTPD Unknown 28.28 0.055 A7 1 ng GAPDH-human Unknown 22.88 0.1 SFTPD Unknown 32.31 0.223 A8 1 ng GAPDH-human Unknown 22.74 0.1 SFTPD Unknown 32.11 0.223 A9 1 ng GAPDH-human Unknown 22.69 0.1 SFTPD Unknown 31.96 0.038 A10 100 pg GAPDH-human Unknown 26.03 0.1 SFTPD Unknown 34.11 0.038 A11 100 pg GAPDH-human Unknown 25.94 0.1 SFTPD Unknown 34.23 0.346 A12 100 pg GAPDH-human Unknown 25.81 0.1 SFTPD Unknown 34.04 0.346 B1 10 pg GAPDH-human Unknown 28.11 0.183 SFTPD Unknown 37.99 0.284 B2 10 pg GAPDH-human Unknown 28.46 0.183 SFTPD Unknown 38.12 0.284 B3 10 pg GAPDH-human Unknown 28.49 0.183 SFTPD Unknown 38.64 0.053 B4 1 pg GAPDH-human Unknown 32.02 0.183 SFTPD Unknown 39.65 0.053 B5 1 pg GAPDH-human Unknown 32.23 0.183 SFTPD Unknown 39.74 0.019 B6 1 pg GAPDH-human Unknown 32.11 0.183 SFTPD Unknown Undetermined B7 No RT GAPDH-human Unknown Undetermined SFTPD Unknown Undetermined B8 NTC GAPDH-human Unknown Undetermined SFTPD Unknown Undetermined B9 B10 B11 B12 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 C12 D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 D11 D12 E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 E11 E12 F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12 G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 H1 H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12