DEVELOPMENTAL PHYSIOLOGY I
 Vitezslav Bryja & Pavel Krejci

Institute of Experimental Biology, Masaryk University, Brno, Czech Republic
Department of Cytokinetics, Institute of Biophysics AVCR, Brno, Czech Republic

•DEVELOPMENTAL BIOLOGY
•- central to all other areas of biology – unites cell biology, genetics and morphology
•- in last 25 years grew into the most exciting and dynamic field of biology…..due to the advances
in three main traditions: the experimental embryology, developmental genetics and molecular
biology. All animals develop similarly, that including the worm, fly, fish or mammal.
•- significant impact on society, in vitro fertilization, teratology, birth defects.
•- future impact: functional genomics and functional proteomics, disease therapy, prenatal
screening, transplantation, embryonal stem cells, therapeutic cloning.
•
AMAZING NATURAL SPECTACLE AND A SOURCE
OF INSPIRATION FOR ALL OTHER AREAS OF BIOLOGY




YSL – yolk syncytial layer






hypoblast
epiblast


- cell nuclei visualized using GFP-tagged histone-2B injection
- 400000 images per embryo taken
-app.18000 cells individually tracked in the 18-hours-old embryo
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SCIENCE 2008, 322, 1065-9