Product Data Sheet
PE anti-mouse CD133
Mouse CD133 transfected RBL-2H3
cells were stained with CD133 (clone
315-2C11) PE (filled histogram) or rat
IgG2a, κ PE isotype control (open
histogram).
C57BL/6 mouse bone marrow cells
were stained with FITC-conjugated
Ly-6G, CD3 and B220, CD117 APC
and CD133 (clone 315-2C11) PE
(middle) or rat IgG2a, κ PE (bottom)
isotype control. Data was gated on
Ly-6G/CD3/B220 negative cell
population.
Catalog # / Size: 141203 / 25 µg
141204 / 100 µg
Clone: 315-2C11
Isotype: Rat IgG2a, λ
Immunogen: CFA
Reactivity: Mouse
Preparation: The antibody was purified by affinity chromatography and conjugated with PE
under optimal conditions. The solution is free of unconjugated PE and
unconjugated antibody.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Concentration: 0.2 mg/ml
Storage: The antibody solution should be stored undiluted at 4°C and protected from
prolonged exposure to light. Do not freeze.
Applications:
Applications: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent
staining with flow cytometric analysis. For immunofluorescent staining, the
suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It
is recommended that the reagent be titrated for optimal performance for each
application.
Description: CD133 (also known as Prominin-1 or AC133) is a 120 kD pentaspan
glycoprotein with 5 transmembrane domains which localize to cellular
protrusions. CD133 was initially described as a surface antigen specific for
human hematopoietic stem cells and as a marker for murine neuroepithelial
cells and some embryonic epithelia. Later on, CD133 was found on other
stem cells including endothelial progenitor cells, glioblastomas, neuronal, and
glial stem cells. In addition to hematopoietic stem cells, CD133 was found on
cancer cells, such as some leukemia cells and brain tumors cells. It also has
been detected at low levels in the kidney, pancreas, placenta, and fetal liver
tissue. Although the biological function of CD133 is not conclusive, CD133
has been extensively used as a stem cell marker for normal and cancerous
tissues.
Antigen References: 1. Singh SK, et al. 2004. Nature 18:396.
2. Falini B, et al. 2005. New Engl. J. Med. 352:254.
3. Bonanno G, et al. 2004. Transfusion 44:1087.
4. Gaipa G, et al. 2002. Haematologica 87:347.
5. Wuchter C, et al. 2001. Haematologica 86:154.
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