Product Data Sheet
APC/Cy7 anti-mouse/human CD44
C57BL/6 splenocytes stained with
IM7 APC/Cy7
Catalog # / Size: 103027 / 25 µg
103028 / 100 µg
Clone: IM7
Isotype: Rat IgG2b, κ
Immunogen: Dexamethasone-induced myeloid leukemia M1 cells
Reactivity: Mouse, Human, Cross-Reactivity: Baboon, Chimpanzee, Cynomolgus,
Rhesus, Horse (Equine), Cattle (Bovine, Cow), Swine (Pig, Porcine), Dog
(Canine), Cat (Feline)
Preparation: The antibody was purified by affinity chromatography, and conjugated with
APC/Cy7 under optimal conditions. The solution is free of unconjugated
APC/Cy7 and unconjugated antibody.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Concentration: 0.2 mg/ml
Storage: The antibody solution should be stored undiluted at 4°C and protected from
prolonged exposure to light. Do not freeze.
Applications:
Applications: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For
immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is
recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD44. Additional
reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and
formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, and in vivo
inhibition of DTH4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is
recommended for functional assays (Cat. No. 103014).
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon
University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed
for research use only.
Application References: 1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, C')
2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
8. Lee JW, et al. 2006. Nature Immunol. 8:181.
9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
12. Wang XY, et al. 2008. Blood 111:2436. PubMed
13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
14. Yamazaki J, et al. 2009. Blood PubMed
15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
Description: CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes,
endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory
stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a
valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent
suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to
and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte
aggregation.
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible
for patent infringement or other violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may
not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By
use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not
intended for human or animal diagnostic, therapeutic or commercial use.
BioLegend 11080 Roselle Street, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 455-9588 Fax: (858) 455-9587
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.
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TruStain fcX™ (anti-mouse CD16/32) 93 FC
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible
for patent infringement or other violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may
not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By
use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not
intended for human or animal diagnostic, therapeutic or commercial use.
BioLegend 11080 Roselle Street, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 455-9588 Fax: (858) 455-9587