Product Data Sheet
PE Rat IgG2a, κ Isotype Ctrl
Catalog # / Size: 400507 / 25 µg
400508 / 100 µg
Clone: RTK2758
Isotype: Rat IgG2a, κ
Immunogen: Trinitrophenol + KLH
Reactivity: KLH
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution
is free of unconjugated PE and unconjugated antibody.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Concentration: 0.2 mg/ml
Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not
freeze.
Applications:
Applications: FC, ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as
negative control. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: The RTK2758 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting,
immunoprecipitation, immunohistochemistry, functional assay, immunofleorescence microscopy,
immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF
™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays
(Cat. No. 400516) as negative control.
Application References: 1. Nishimoto H, et al. 2005. Blood 106:4241.
2. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed
3. Schmidt NW, et al. 2011. J. Immunol. 186:5873. PubMed.
Description: The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen
as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible
for patent infringement or other violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may
not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By
use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not
intended for human or animal diagnostic, therapeutic or commercial use.
BioLegend 11080 Roselle Street, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 455-9588 Fax: (858) 455-9587