Innovation Lectures (INNOLEC)


Solid-State Light Sources in Chemistry and Science: Utilizing the Benefits of Light Emitting Diodes
    and Laser Diodes as the Light Sources of the 21st Century in Chemical Analysis, Detection,
                Fluorescence Microscopy, Visualization, Photochemistry and Teaching


                                         Prof. Mirek Macka

      Australian Centre for Research on Separation Science (ACROSS) and School of Chemistry,
                  University of Tasmania, Private Bag 75, Hobart 7001, AUSTRALIA

                                  e-mail: mirek.macka@utas.edu.au



                               Mon 3.9. – Tue 4.9. 2012 13:00-17:00


                                Masaryk University Campus Bohunice



This interdisciplinary short course is an intensive introduction to the properties and usage of
light emitting diodes (LEDs) and diode lasers (LDs) in chemical analysis and chemistry in general.
The course will cover the background and theory and then will focus on practical issues and the
applications of LEDs particularly for analytical optical methods (photometry, fluorimetry), optical
detection in analytical flow-through methods (FIA, HPLC, CE), optical sensors, microfluidic chips,
fluorescence microscopy and visualization in photochemistry and teaching.  Examples of some
elementary techniques used to characterise LEDs including measurement of emission spectra will be
conducted as hands-on experiments.


Course text: Class notes; readings to be assigned.

Reference texts and websites:

Gilbert Held, Introduction to Light Emitting Diode Technology and Applications, CRC Press, Boca
Raton, 2009.

http://en.wikipedia.org/wiki/Light-emitting_diode

http://www.ledsmagazine.com/

http://ca.pittcon.org/Technical+Program/tpabstra10.nsf/SCoursesByCat/CB1DD8E4B5476AD7852575E700803F
47?opendocument

http://electronics.howstuffworks.com/led.htm
http://www.viewsfromscience.com/documents/webpages/led_fluorescence.html
http://www.microscopy-analysis.com/features/led-light-sources-major-advance-fluorescence-microscopy

http://www.roithner-laser.com/


Kontaktní osoba: Prof. Viktor Kanický, Ústav chemie, kl. 4774; e-mail: viktok@chemi.muni.cz

                                             Syllabus



Lectures 1 and 2


  * Why use LEDs in chemistry and science?
  * History
  * Fundamentals (physical principles, construction)
  * Basics of LEDs in respect to their applications in analysis and chemistry
  * Application areas
  * Considerations in choosing a LED
  * Practical issues when using LEDs
  * Coupling of LEDs with optical fibers
  * Pulsed techniques: use of lock-in amplifiers and LEDs in TRF (time resolved fluorescence)
Optical methods in chemical analysis with an emphasis on photometry and photometric detection,
fluorimetry and optical detection methods in microfluidic chips
  * Basics of construction of simple LED-based photometers and fluorimeters
  * Fluorescence microscopy and visualization using a set-up for <@250
  * Diode lasers for compact inexpensive LIF (Laser Induced Fluorescence) detection in on capillary
and microfluidic chip formats
  * LEDs as light sources for photochemistry
  * Other advanced and specialized methods
  * Outline of hands-on experiments: measuring emission spectra, optical power, intensity,
transmittance, absorbance, pulsing LEDs and use of lock-in amplifier techniques
  * Workshop on examples from participants’ own specific usage, questions, discussion

  * Workshop using examples incl. particular applications of course participants
  * Consultations