Task 1 16 October 2012
Determination of oligonucleotide concentration
You have obtained envelope from your favorite supplier. The envelope contained dried
primer with sequence 5´-CTCTgATggTTCACTggATC-3´.
The synthesis protocol says that the total amount of DNA is approximately 10 µg.
After dissolving the whole amount in 1 mL of TE buffer,
you measured exact absorbance A at 260 nm.
Absorbance values A can be found in the table next to your name.
1) What is the precise molar concentration of DNA in µM (10-6
M)?
2) How much was the light intensity reduced after going through cuvette comparing the
intensity of incident light? Report the transmitted light intensity as a percentage of the
incident light intensity.
For determination of oligonucleotide characteristics use calculator at
http://eu.idtdna.com/analyzer/Applications/OligoAnalyzer/
Please send me your short answers via email within 48 hours.
Correct answer = 0.5 point
A
1 Bencúrová, Petra 0.340
2 Dabravolski, Siarhei 0.360
3 Dubec, Vít 0.380
4 Dudová, Zdenka 0.400
5 Dvořák, Jan 0.420
6 Fabišik, Matej 0.440
7 Fedorko, Jan 0.460
8 Fialová, Martina 0.480
9 Holek, Michal 0.500
10 Kočka, Martin 0.520
11 Míka, Matěj 0.540
12 Obacz, Joanna Agnieszka 0.560
13 Partyka, Jan 0.580
14 Přikrylová, Terézia 0.600
15 Rájecký, Michal 0.620
16 Reichman, Pavel 0.640
17 Sochorová, Jana 0.660
18 Škubník, Karel 0.680
19 Tylichová, Zuzana 0.700