Study of chemical systems by means of luminescence spectrometry


                                           Petr Táborský


Luminescence spectrometry is powerful analytical method of studying material that can be made to
fluoresce, either in its natural form (native/intrinsic luminescence) or when treated with
chemicals capable of fluorescing (extrinsic luminescence). The extrinsic luminescence is from
analytical point of view usually divided into labels (covalent bond) and probes (non-covalent
interaction). Optical properties of the probes are usually effected (solvent effect, quenching,
energy transfer, etc.) by surrounding molecules and vice versa measured changes (luminescence
intensity, emission maxima, life-time, quantum yield, etc.) can give information about the probe
environment. The lecture will show two examples of luminescent probes: lanthanide(III) based probes
and DNA probes derived from Quaternary benzo(c)phenanthridine alkaloids (QBAs).