The Plant Cell, Vol. 5, 1337-1348, October 1993 © 1993 American Society of Plant Physiologists
Gametes and Fertilization: Maize as a Model System for Experimental Embryogenesis in Flowering Plants
Christian Dumas9'1 and H. Lloyd Mogensenb
a UMR CNRS-INRA 9938, Ecole Normale Superieure de Lyon, 46 allee d'ltalie, 69364 Lyon Cedex 07, France b Department of Biological Sciences, Box 5640, Northern Arizona University, Flagstaff, Arizona 86011
INTRODUCTION
The involvement of each of the two sperm cells from a pollen tube in separate fusion events characterizes double fertilization: a process that is illustrated in Figure 1. This process, which initiates the development of both the embryo (via the fusion of sperm and egg) and the endosperm (via the fusion of sperm and central cell), was discovered in angiosperms nearly a century ago (Nawaschin, 1898; Guignard, 1899; reviewed in Russell, 1992).
The application of the transmission electron microscope to the study of flowering plant embryogenesis in the early 1960s by Jensen and coworkers (reviewed in Jensen, 1973, 1974) marked the beginning of great strides that have been made in our understanding of embryogenesis since Maheshwari's (1950) comprehensive review. Jensen and Fisher (1968,1970) confirmed the cellular nature of sperm cells and noted that sperm cells remain connected after their formation from the generative cell division. Jensen (1973,1974) also showed that the two synergids that typically flank the egg are active, functional cells that play a significant role in the fertilization process. More recent studies, also employing transmission electron microscopy but extending its use to the creation of three-dimensional, computer-generated reconstructions, have led to the concept of the male germ unit, which views the sperm cells and vegetative nucleus as a functional assemblage required for normal gamete transport, recognition, and fusion (see below; Dumas etal., 1984; reviewed in Mogensen, 1992). Because the sperms of a pair are dimorphic in some species, it also became apparent that double fertilization may not be a random event, i.e., that each sperm may be preprogrammed for fusion with either the egg or the central cell (Russell, 1985).
Although the process of double fertilization offers unique opportunities for studying gamete interactions and control mechanisms, it also presents special problems not encountered in animals and nonangiospermous plants. A major deterrent to physiological, molecular, and experimental studies of embryogenesis in angiosperms has been the inaccessibility of the gametes. During the past few years, however, it has become possible in some angiosperm species to isolate male and female sex cells in large enough quantities to allow for the initial phases of such analyses.
1 To whom correspondence should be addressed.
Although sperm cell isolation has been accomplished in several species (for reviews, see Russell, 1991; Theunis et al., 1991; Chaboud and Perez, 1992), female gamete isolation has been less successful in most of these species. In maize, it is possible to isolate large numbers of both gamete types; for this reason and others, enumerated below and in Table 1, maize is emerging as a model system for the study of gametes, fertilization, and experimental embryogenesis. Thus, we have focused this review primarily on recent studies in maize, with reference to other systems where appropriate.
SUITABILITY OF MAIZE FOR THE STUDY OF GAMETES, FERTILIZATION, AND EXPERIMENTAL EMBRYOGENESIS
As shown in Figure 2, the unisexual flowers of maize occur separately in the form of the tassel (staminate flowers; Figure 2A) at the tip of the plant and one or more ears (pistillate flowers; Figure 2F) in the axils of leaves near the middle of the stem. Several million pollen grains are released from the tassel, and 400 to 500 ovules are available per ear. Controlled pollination is much easier in maize than in other cereals, in which emasculation of each flower is required before it can be used as a female parent.
In addition to these useful sexual traits, considerable data are available on the genetics and cytogenetics of maize (Sheridan, 1982). More recently, much attention has been focused on molecular approaches to the study of maize development, including the use of restriction fragment length polymorphism markers (more than 1200 are known today) and on the analysis of some interesting genes and their protein products (Sheridan, 1988; Vollbrecht etal., 1991; Bellmann and Werr, 1992; Veit et al., 1993, this issue).
Recent Progress in Gamete Isolation and Manipulation
The course of contemporary research in sperm cell biology was plotted at the first sperm cell biology conference, held in Flagstaff, Arizona, in 1985. This conference led to the
1338      The Plant Cell
Figure 1. Double Fertilization in Maize.
The pollen tube (pt) has entered the degenerated synergid (ds) and discharged through a terminal pore. The two sperm cells have traversed the synergid and their nuclei (unlabeled arrowheads) are seen fusing with the egg (e) nucleus (yellow) and the polar nuclei (red) within the central cell (cc). ii, inner integument; n, nucellus. Adapted with permission from Van Lammeren (1986) according to Ft. Mol and C. Dumas' unpublished results on genotype A 188.
development of several procedures for isolating en masse viable sperm cells of maize, as depicted in Figures 2A to 2E (Dupuis et al., 1987; Yang and Zhou, 1989) and for characterizing them (Russell et al., 1990; Roeckel et al., 1991), as shown in Table 2. Table 3 shows that a parallel approach has been developed to successfully isolate living embryo sacs and egg cells for further manipulation and characterization (Wagner et al., 1989b; Figures 2F to 2J). Several recent reviews (Roeckel et al., 1991; Theunis et al., 1991; Chaboud et al., 1992; Russell, 1993, this issue) have addressed this topic and its influence on new developments in the study of fertilization in an-giosperms (see also reviews in Russell and Dumas, 1992).
Procedures for the Regeneration of Fertile Plants from "Artificial" and "Natural" Zygotes
Recently developed techniques for the isolation and manipulation of male and female gametes offer a new way to study fertilization through the creation of zygotes induced by elec-trofusion, as illustrated in Figures 3A and 3B (Kranz et al., 1991a, 1991b). In addition, after several years of trials, a critical step has been reached with the possibility of regenerating fertile plants from embryo sacs isolated at the zygote stage
(Campenot et al., 1992; Mol et al., 1993). This progress in gamete technology and in the isolation and regeneration of both "artificial" and "natural" zygotes provides a promising approach for genetic transformation of maize, which is difficult to obtain by other methods (e.g., Gordon-Kamm et al., 1990; D'Halluin et al., 1992). In addition, such zygotes are very powerful tools for the molecular analysis of fertilization and the earliest steps of embryogenesis.
FROM POLLEN TO GAMETE ISOLATION
Pollen Organization and the Male Germ Unit
The pollen grain is a unique multicellular organism. In most angiosperms, it consists of a generative cell (sperm mother cell) surrounded by the vegetative cell (future pollen tube); thus, it is termed "bicellular" pollen. Maize pollen is termed "tricel-lularf because at pollen maturity the generative cell has already divided to form two sperm cells (Bedinger, 1992, Figure 2C).
Many studies conducted over the past decade have documented that the sperm cells either remain connected or become reunited after generative cell division and that one or both sperms forms a close association with the vegetative nucleus. Because the nuclear and cytoplasmic DNA-contain-ing compartments of male heredity are physically linked, they have been termed the male germ unit (Dumas et al., 1984; Mogensen, 1992). Clear evidence of the validity of this concept has been provided by the isolation of the intact male germ unit (Matthys-Rochon et al., 1987). The male germ unit is established before or shortly after pollination, and its components typically maintain their association up to the time of pollen tube discharge into one of the two synergids that flank the egg cell (Russell, 1992; Figure 1). Thus, the male germ unit appears to function as a vehicle for gamete transmission, recognition, and fusion during double fertilization (see reviews in Mogensen, 1992; Russell, 1992, 1993, this issue). However, the male germ unit is usually disrupted during the process of sperm cell isolation (with a notable exception in Brassica; see Matthys-Rochon et al., 1987), and the sperm cells are found singly in the isolation medium. This special association of three different haploid cells may result from the expression of several sets of pollen-specific genes (Mascarenhas, 1990; McCormick, 1991) and from interactions between anther-specific gene products and the innermost layer of the anther, the tapetum (Koltunow et al., 1990).
Because in some plants, the two sperms of a pair differ greatly from one another in size, shape, and organellar content, it was suggested that they may be preprogrammed to fuse with either the egg or the central cell. Such preferential fertilization has been demonstrated to occur in Plumbago, in which the smaller, plastid-rich sperm almost always fertilizes the egg cell (Russell, 1985; also, see Russell, 1993, this issue). The sperms of a pair in maize do not differ greatly from each other
Gametes and Fertilization in Maize
1339
morphologically (McConchie et al., 1987; Rusche, 1988; Rusche and Mogensen, 1988); nevertheless, directed fertilization is known to occur, at least in lines containing B-chromo-somes. In this system, there is a nondisjunction of the B-chromatids during generative cell division, resulting in only one sperm receiving the B-chromosomes. This sperm most often fuses with the egg cell (Roman, 1948; Chaboud et al., 1992).
Pollen Quality: A Prerequisite to Gamete Isolation
Depending on the genotype, a healthy tassel generally sheds several million pollen grains during a 1-week period. Pollen shedding usually begins 1 or 2 days before silks appear on the ear shoots.
The pollen grain is virtually a dehydrated organism, and its "quality" has been defined as its ability to fertilize and produce
Table 1. Maize: Model System for Cellular and Molecular Analysis of Gametes, Fertilization, and Early Embryogenesis
Interesting Traits
Characterization
References
Pollen Pollen collection Long-term pollen storage (LN2) Pollen from in vitro-cultured tassel In vitro pollen maturation from microspore In vitro pollen tube growth In vitro pollen tube growth velocity Ovule number per spike Ovule and silk receptivity In vitro ovule/pollination/regeneration
Pollination/Fertilization Single pollen grain pollination Embryo sac isolation Egg cell isolation Central cell isolation "Artificial zygote" formation "Artificial zygote" regeneration Fertilized embryo sac regeneration
Nonzygotic embryogenesis Somatic embryogenesis Androgenic embryogenesis Gynogenetic embryogenesis
Regeneration/Transformation Microinjection in microspore Microinjection in pollen Microinjection in embryo sac
Transformation
Regeneration from protoplasts
Other various traits Insertional mutagenesis Embryo mutants
Long-term storage of embryos Endosperm mutants
B-A translocations
Several million/inflorescence
Possible in many genotypes
Available
Difficult
Available
5 to 8 mm/hr
400 to 500
Assessed by silk length Available
Available
Available
Difficult
Difficult
Electrofusion
Available
Available
Difficult; genotype dependent Difficult; genotype dependent Difficult; genotype dependent
Available Available Available
Established, but difficult Difficult; genotype dependent
Available Numerous
Available Numerous
Available
Sheridan (1982) Barnabas (1985) Parredy et al. (1989) Dupuis and Pace (1992) Pfahler (1967) Dupuis and Dumas (1989) Sheridan (1982) Dupuis and Dumas (1989) Dupuis (1992)
Kranz and Lorz (1990) Wagner et al. (1989b) Wagner et al. (1989b) Wagner et al. (1989b) Kranz et al. (1991a, 1991b) Kranz and Lorz (1993) Campenot et al. (1992); Mol etal. (1993)
Freeling and Walbot (1993) Freeling and Walbot (1993) Freeling and Walbot (1993)
Gaillard et al. (1992) Kranz and Ldrz (1990) E. Matthys-Rochon
(unpublished results) Gordon-Kamm et al. (1990);
D'Halluin et al. (1992) Rhodes et al. (1988)
Freeling and Walbot (1993) Clark and Sheridan (1991);
Meinke (1991); Walbot (1991) Delvallee et al. (1989) Clark and Sheridan (1991);
Meinke (1991); Walbot (1991);
Freeling and Walbot (1993) Sheridan (1982);
Freeling and Walbot (1993)
Figure 2. Steps in the Isolation of Male and Female Gametes of Maize.
Gametes and Fertilization in Maize 1341
Table 2. Maize Sperm Cells: Technical Characteristics for Cellular and Molecular Analysis
Feature
Characterization
References
Pollen diameter
Sperm cell (SC) diameter
SC volume
SC volume/pollen volume Volume of total SC organellesa/SC volume SC volume/pollen plasma membrane volume
Volume of SC nucleus/SC volume Volume of SC plastids 3-dimensional reconstruction and
characterization of SC "En masse" SC isolation SC-enriched fraction SC survival
Long-term SC storage medium
SC analysis by cell sorter
SC isolation procedure from pollen tube
SC cDNA library
Isolation of SC from B-chromosome line SC cytology in vivo
100 tim 5 to 7 urn
163 to 253 urn3 0.002% to 0.2% 35.78
0.5% to 2%
32.34
0
Available
106 to 107; 80 to 90%b
Available
72 hr at 20°C
Available
Available
Not available
Available
Available
Documented
M. Rusche (personal communication)
Cass and Fabi (1988); Wagner et al. (1989a);
Mogensen et al. (1990) Wagner et al. (1989a); Mogensen et al. (1990) Mogensen et al. (1990) Mogensen et al. (1990)
Mogensen et al. (1990) Mogensen et al. (1990) Mogensen et al. (1990)
Mogensen et al. (1990) Dupuis et al. (1987); Zhang et al. (1992b) Chaboud and Perez (1992); Chaboud et al. (1992) Zhang et al. (1992a)
Chaboud and Perez (1992); Chaboud et al. (1992) Zhang et al. (1992b)
A. Chaboud and C. Breton (unpublished results) A. Chaboud, H. L. Mogensen, and C. Dumas (unpublished results) McConchie et al. (1987); Rusche (1988); Rusche and Mogensen (1988)
a Excluding vacuoles.
b Total number of isolated SC obtained from a single isolation procedure; percentage of isolated cells recovered.
seeds. However, the water content of living pollen grains varies considerably among different families, with most values lying between 15 and 35% of the fresh weight at anthesis, with some extremes of 8% water in poplar pollen and 60% water in maize pollen. Although it is easy to evaluate pollen viability, pollen quality is much more difficult to assess by routine tests. In
maize, pollen longevity is very short under natural conditions, from 20 min to 2 hr. The most rapid method available to determine pollen viability is a cytological technique, the fluorochromatic reaction (FCR) (Heslop-Harrison and Heslop-Harrison, 1970), which indicates the structural integrity of the plasma membrane.
Figure 2. (continued).
(A) to (E) Isolation of male gametes.
(A) Tassels containing staminate flowers.
(B) Collection of pollen on an aluminum sheet.
(C) Schematic of a maize pollen grain containing two sperm cells. Bar = 40 urn.
(D) Centrifuge tubes with purified sperm cell fraction in Percoll gradient.
(E) Computer-assisted reconstruction of an isolated sperm cell based on serial ultrathin sections. Reproduced with permission from Mogensen et al. (1990). Bar = 5 urn.
(F) to (J) Isolation of female gametes.
(F) Young ear of maize with pistillate flowers.
(G) Longisection of a floret showing the position of the embryo sac within the ovule. Reproduced with permission from Wagner et al. (1990). Bar = 500 urn.
(H) Longisection of the embryo sac showing the egg, central cell, and synergid. Reproduced with permission from Wagner et al. (1990). Bar = 50 urn.
(I) Isolated egg cell and two synergids. Bar = 50 urn.
(J) Computer-assisted reconstruction of an isolated egg cell based on serial ultrathin sections. Reproduced with permission from Faure et al. (1992). Bar = 10 urn.
sc, sperm cells; es, embryo sac; e, egg; cc, central cell; sy, synergid.
1342      The Plant Cell
Table 3. Isolation and Characterization of the Embryo Sac and Female Gametes
Type of Cell
Data
References
Embryo sac Embryo sac isolation Cytology of embryo sac
Embryo sac localization, 3-dimensional reconstruction Embryo sac microinjection Embryo sac cDNA library
Egg cell Egg cell isolation
Isolated egg cell (3-dimensional reconstruction)
Egg cell longevity
Egg cell volume/sperm cell volume
Volume of egg nucleus/egg volume
Volume of total egg organellesa/egg volume
Volume of egg plastids/egg volume
Egg cell cDNA library
Synthesis and accumulation of ribosomes
Central cell Central cell isolation
Isolated central cell (3-dimensional reconstruction)
Central cell cDNA library
Synthesis and accumulation of ribosomes
Available Available
Available Available Not yet available
Difficult Available
Two weeks at 24°C
300
1.71%
8.98%
2.95
Not yet available Studied
Difficult
Not yet available Not yet available Available
Wagner et al. (1989b)
Diboll and Larsen (1966); Diboll (1968);
Russell (1979); Van Lammeren (1986) Wagner et al. (1990) E. Matthys-Rochon (unpublished results)
Wagner et al. (1989b) Faure et al. (1992) Kranz et al. (1991a) Faure et al. (1992) Faure et al. (1992) Faure et al. (1992) Faure et al. (1992)
Dow and Mascarenhas (1991a, 1991b)
Wagner et al. (1989b)
Dow and Mascarenhas (1991a, 1991b)
a Vacuoles excluded.
However, a multidisciplinary approach that includes FCR testing, seed set, in vitro germination, freeze fracturing of pollen grains, and a nondestructive method, nuclear magnetic resonance, is necessary to follow pollen quality effectively during aging. To date, such an approach has been used only with maize pollen. A strict correlation has been found between maize pollen water content and plasma membrane integrity and pollen viability. During pollen aging, a natural dehydration occurs that induces major modifications in the plasma membrane, and beyond a critical point, there is a dramatic modification of the water content, as determined by characteristic values of proton mobility. At this point, the pollen loses its quality (Kerhoas et al., 1987). These data partially explain why pollen is especially sensitive to environmental stresses such as drying and temperature (Sheridan, 1982), and why it is so difficult to store pollen in liquid nitrogen (Barnabas, 1985; C. Digonnet-Kerhoas, G. Gay, and C. Dumas, unpublished results).
There is little hope for fertilization if the temperature rises much above 36PC. The synthesis of heat shock proteins (HSPs) has been analyzed in male and female tissues to establish a relationship between physiological and molecular responses to heat shock. Under heat shock conditions (up to 36°C), the synthesis of a typical set of HSPs is induced in the female tissues. HSPs are also normally inducible during microsporo-genesis but not in mature pollen, which at these temperatures is unable to effect fertilization (Dupuis and Dumas, 1990a).
Approaches have been developed to study pollen formation under controlled conditions, such as the in vitro culture
of maize tassels, a technique that has been used successfully in several genotypes (Parredy and Greyson, 1985; Parredy et al., 1989). Another approach, which, so far, has had limited success, involves maturation of pollen in vitro from cultured microspores (Dupuis and Pace, 1992).
Sperm Cell Isolation Procedures
Isolated sperm cells can easily be obtained in large numbers from maize pollen (an average of 106 to 107 cells are obtained per isolation procedure) by osmotic shock coupled with a pH shock (Dupuis et al., 1987). The sperm cells are then separated from pollen contaminants by filtration and discontinuous Percoll gradient centrifugation (Figures 2B to 2E). After isolation, sperm cell viability is generally evaluated with the aid of the FCR. An improved isolation procedure has recently been published (Roeckel et al., 1991). In addition, recent attempts have been made to develop a long-term sperm cell storage medium for further molecular analysis (Roeckel et al., 1991; Zhang et al., 1992a, 1992b).
Physiological Characterization of Sperm Cells
Electron microscopy has demonstrated that isolated sperm cells of maize are true protoplasts, i.e., intact cells without a cell wall (Dupuis et al., 1987; Cass and Fabi, 1988; Wagner et al., 1989a). This has recently been confirmed by preliminary
Gametes and Fertilization in Maize 1343
patch-clamp measurements (A. Chaboud, M. de Barros Lopes, C. Breton, R. Pidulsky, O. Rougier, and C. Dumas, unpublished results). Other studies have shown that the presence of ATP (a nucleotide known to be an indicator of life and used to test the fertility of human sperm) is a good indicator of the metabolic potential of these cells (Roeckel et al., 1991; Chaboud and Perez, 1992). In addition, ^S-methionine labeling experiments clearly show that these isolated cells are able to synthesize new proteins (P. Roeckel and C. Dumas, unpublished results).
A
Figure 3. In Vitro Fertilization in Maize Facilitated by Electrofusion.
(A) Isolated sperm and egg cells are placed into a fusion droplet (light blue), dielectrophoretically aligned with electrodes (green), and electrically fused (adapted with permission from Kranz et al., 1991a).
(B) Sequence of electrofusion between isolated egg (e) and sperm (sc) cells. Arrowheads show entrance site of the sperm cell. The time interval, after the DC pulse, from 1 to 3 was less than one second. Reproduced with permission from Kranz and Lorz (1991). Bar = 50 urn.
The inner plasma membrane of the vegetative cell has been shown to surround the sperm cell plasma membrane by both conventional transmission electron microscopy (McConchie et al., 1987; Rusche, 1988; Rusche and Mogensen, 1988) and the freeze-fracture method (Southworth, 1992). During in vitro isolation, this vegetative inner membrane is lost, a phenomenon that also occurs during pollen tube discharge within a synergid prior to fertilization. Such a phenomenon has been compared to capacitation in animal gametes, which prepares sperm cells to fuse with the female gametes (Dumas et al., 1984; Russell, 1993, this issue).
Gamete Dimorphism
Success in isolating intact, viable, and functional maize gametes offers the possibility of identifying the specific determinants involved in fertilization, which are thought to be borne on the interacting gamete plasma membranes (Dumas et al., 1984; Chaboud et al., 1992). Such studies may uncover differences in the membrane proteins of dimorphic sperm cells. The construction of a monoclonal antibody library directed against the surface of viable sperm cells has been designed (Hough et al., 1986) and developed in maize as well as in Brassies campestris and Plumbago zeylanica (reviewed in Chaboud and Perez, 1992). However, a common problem is that the strongly reactive lines are directed to debris or cell contaminants rather than to plasma membrane surface determinants. These studies clearly indicate the necessity of obtaining more highly purified gamete preparations, free from pollen cytoplasmic contaminants (Chaboud et al., 1992). However, if the purity of the fraction is increased significantly, the number of pure living sperm cells or amount of pure plasma membrane fraction decreases dramatically (see Table 2). In parallel, in vitro fertilization of isolated gametes without the aid of electrofusion or polyethylene glycol has recently been developed (J.-E. Faure, C. Digonnet-Kerhoas, and C. Dumas, unpublished results). This system will allow selection of fusion-inhibiting monoclonal antibodies and, thus, the isolation of putative candidate molecules involved in gamete recognition (A. Chaboud and C. Dumas, unpublished results).
Nuclear dimorphism in maize sperms, in the form of B-chro-mosomes usually being present in only one of the sperms (Roman, 1948), is a potentially useful trait for separating one type of sperm from the other. However, preliminary trials using flow cytometry suggest that detection of DNA differences between isolated sperms would require ~10 B-chromosomes (H. L. Mogensen and A. Chaboud, unpublished results). Unfortunately, when the number of B-chromosomes is this high, their distribution to the sperms becomes random (Carlson, 1988). There are small structural differences between the sperm pair of maize even in the absence of B-chromosomes (McConchie et al., 1987; Rusche, 1988; Rusche and Mogensen, 1988), but these differences do not allow the detection of two sperm types within a population of isolated sperm cells (Wagner et al., 1989a; Mogensen et al., 1990).
1344     The Plant Cell
FROM OVULE TO FEMALE GAMETE ISOLATION
In angiosperms, the embryo sac represents the female gametophytic generation (see Reiser and Fischer, 1993, this issue). This megagametophyte is deeply embedded in the protective sporophytic envelope of the ovary and ovule as a consequence of angiospermy (Figures 2G and 2H). The female germ unit (Dumas et al. 1984; Huang and Russell, 1992) is seen as including the egg apparatus (the egg and syner-gids) and the central cell because in most species, all of these components are involved in the fertilization process, which includes pollen tube attraction to the ovule and one synergid, pollen tube discharge, sperm cell transport, and gamete fusion (Figure 1; Russell, 1992). The special environment within the penetrated synergid, which typically degenerates before pollen tube arrival, is undoubtedly critical to the normal process of syngamy. For instance, the calcium concentration in the synergids is high. Simulating these conditions will be a major but necessary challenge to achieve fertilization in vitro without the application of electric pulses.
One consequence of the fact that the embryo sac is embedded deep within maternal tissues is that it is difficult or impossible to make physiological measurements and direct morphological observations. Several methods have been developed for isolating embryo sacs from ovular tissues, including partial enzymatic digestion of ovules followed by manual dissection, squashing (Zhou, 1985; Wagner et al., 1989b), or microdissection (Mol et al., 1993). Using these methods, it is possible to obtain viable embryo sacs and, after an additional, very brief enzymatic treatment, living gametes (both egg, Figures 21 and 2J, and central cells) can be separated (Wagner et al., 1989b). The debris is removed by filtration through several nylon filters, and embryo sacs and gametes are then placed in the appropriate survival medium.
Studies using a molecular approach to investigate the components of the maize embryo sac are just getting underway (Reiser and Fischer, 1993, this issue). As part of a project to isolate genes expressed in the female gametophyte, Dow and Mascarenhas (1991a, 1991b) have employed in situ nucleic acid hybridization to determine the relative number of ribosomes within the cells of the embryo sac of maize. In the mature embryo sac, the central cell contains ~200 times as many ribosomes as are present in a nucellar cell, ~7 times as many ribosomes as are contained in the egg cell, 14 times as many ribosomes as in a synergid, and ~80 times as many ribosomes as in each antipodal cell. During embryo sac maturation, the accumulation of ribosomes appears to proceed at a constant and high rate, with the highest rate in the developing central cell.
Mature embryo sacs must be isolated from receptive ovules, that is, ovules at a stage during which pollination will be effective. However, ovule receptivity is a complex and unclear physiological parameter that is still largely ignored and difficult to assess. In maize, female receptivity can be estimated by the total silk length, which corresponds to the maturation state of spikelets (Dupuis and Dumas, 1990b). Polyacrylamide
gel electrophoresis of silk proteins shows that ~10 proteins seem to be correlated with the acquisition of silk receptivity (Dupuis, 1992). In addition to these putative protein markers, the level of expression of a gene coding for a serine/threonine protein kinase (Walker and Zang, 1990) seems to be positively correlated with silk receptivity (R. Perez, A. Chaboud, and C. Dumas, unpublished results).
EGG CELL CHARACTERIZATION
The egg cell of maize has been well characterized cytologi-cally at both the light and electron microscope levels (Table 3; Russell, 1993, this issue). Unique features include the presence of highly elongated, branched mitochondria and a large, chalazally positioned vacuole (Diboll and Larson, 1966; Diboll, 1968; Van Lammeren, 1986; Wagner et al., 1990). Upon isolation, the egg protoplast is spherical, with numerous small vacuoles surrounding the centrally located organelles, among which the nucleus is positioned near the periphery (Faure et al., 1992; Figures 21 and2J). Until it is possible to isolate maize eggs in larger numbers, characterization of these cells at the molecular (e.g., cDNA libraries) and physiological (ATP production, protein synthesis, etc.) levels will be somewhat restricted.
IN VITRO POLLINATION: INTEREST AND LIMITS
The reproductive phase of the plant life cycle is difficult to analyze in the field because of the number of factors involved: among these are female receptivity, pollen quality, and environmental influences. An in vitro pollination system can bypass many of these variables; such an approach, followed by embryo rescue, has generally been used to obtain wide hybridization in higher plants (Zenkteler, 1990,1992). In vitro pollination in maize (Sladký and Havel, 1976; Gengenbach, 1977) is typically performed by collecting unpollinated ears from the plant; segments of the cob, with two adjacent rows of ovaries attached, are then dissected under sterile conditions. These segments are placed on nutrient agar in a Petri dish, and the silks are pollinated. This technique allows the measurement and experimental manipulation of pollen tube growth within the silk as well as an appreciation of the kinetics of fertilization (Dupuis and Dumas, 1989; Booy et al., 1992). Successful fertilization has been obtained with as few as one pollen grain per silk (Kranz and Lorz, 1990).
The in vitro pollination technique has also been used successfully to separate pollen and ovule behavior (e.g., heat stress tolerance; Dupuis, 1992). This technique can be used to classify different genotypes with regard to stress responses (e.g., to temperature, heavy metal, and osmotic stresses), because in vitro conditions are reproducible. In this regard, Higgins and Petolino (1988) have observed a genotype effect on in vitro pollination success.
Gametes and Fertilization in Maize 1345
FROM NATURAL AND ARTIFICIAL ZYGOTES TO PLANT REGENERATION
A major challenge to plant developmental biologists is the establishment of a successful system for studying the processes of morphogenesis following zygote formation. Such a system is readily available in animals (in vitro fertilization) and in lower plants such as algae (Dale, 1991), but, as outlined in Table 4, until recently no progress had been made in flowering plants. The combination of egg and sperm cell isolation with the technical procedures used for fusing plant protoplasts has opened a new window in gamete technology that was not expected only a few years ago (see Chapman et al., 1985).
Artificial zygotes have been developed by Kranz et al. (1991a, 1991b) from electrofusion of enzymatically or mechanically isolated gametes (Figures 2A and 2B; Table 4). Such zygotes have been shown to be capable of developing into fertile, diploid plants possessing characteristics of both parents (Kranz and Lorz, 1993). The kinetics of karyogamy in such zygotes have been analyzed by classic light and electron microscopy, as well as by reembedding semithin sections and three-dimensional computer-aided reconstruction (Faure et al., 1993; Table 4).
Fusion of isolated gametes without electrofusion has recently been achieved. However, in this case, nuclear fusion must be verified, and plant regeneration needs to be carried out (J.-E. Faure, C. Digonnet-Kerhoas, and C. Dumas, unpublished results).
Another possible method to examine the zygote and its products is to isolate the embryo sac just after fertilization, that is, from 6 to 8 hr after pollination, depending on the genotype and the environmental conditions, particularly temperature. Successful isolation strategies have been developed by two groups (Campenot et al., 1992; Mol et al., 1993; Table 4). Embryo sacs were isolated by enzymatic treatment or microsurgical dissection at the zygote or early embryo stage. The "isolated" zygotes and early embryos were surrounded by the cells of the embryo sac as well as some cells of the nucellus. Using this method, it is possible to regenerate complete plants.
CONCLUSIONS
Although double fertilization was first described by Nawaschin in 1898 and a considerable amount of work has been done on this unique feature of flowering plants, including the study of its possible evolutionary origin (Friedman, 1992), very little progress has been made in terms of molecular analysis. New opportunities are now being provided by in vitro gamete isolation procedures (Dumas and Russell, 1992) and by the development of methods for regenerating electrof used zygotes (Kranz and Lorz, 1993), for injecting sperm cells or sperm nuclei into the isolated embryo sac to mimic fertilization (E. Matthys-Rochon, R. Mol, P. Heizmann, and C. Dumas, unpublished results), and for regenerating in vitro zygotes or embryos
Table 4. Artificial and Natural Zygotes and Zygotic Embryogenesis in Maize
Type of Zygote
Property
References
"Artificial zygote" "Artificial zygote" by electrofusion Karyogamy in electrofused zygote cDNA library of "artificial zygote" Fertile plant regeneration from electrofused zygote
"Artificial zygote" without electrofusion
"Natural zygote" From isolated fertilized embryo sac Fertile plant regeneration from isolated
fertilized embryo sacs Microinjection into isolated embryo sacs Fertile plant regeneration from two-celled
proembryos8 Fertile plant regeneration from isolated
embryos (2 DAP)b cDNA library of isolated embryos (5 DAP)b
Available Demonstrated Not yet available
Available Available
Available
Available Available
Available
Available Available
Kranz et al. (1991a, 1991b) Faure et al. (1993)
Kranz and LSrz (1993) J.-E. Faure, C. Digonnet-Kerhoas, and C. Dumas (unpublished results)
Mol et al. (1993)
Campenot et al. (1992); Mol et al. (1993) E. Matthys-Rochon (unpublished results)
Campenot et al. (1992); Mol et al. (1993)
Campenot et al. (1992); Mol et al. (1993) C. Breton (unpublished results)
a From isolated embryo sacs.
b Following natural pollination and fertilization; DAP, days after pollination.
1346     The Plant Cell
isolated from fertilized embryo sacs (Campenot et al., 1992; Mol et al., 1993). cDNA libraries of gametes and early stages of zygotic development (A. Chaboud, M. de Barros Lopes, C. Breton, R. Pidulsky, O. Rougier, E. Matthys-Rochon, and C. Dumas, unpublished results) may provide new tools for the molecular analysis of fertilization and early embryo development.
It may soon be possible to develop genetically transformed maize by microinjecting transformed sperm into the embryo sac rather than by using transformed pollen grains (Roeckel et al., 1992). The latter procedure has failed because of the presence of very active nucleases within the pollen wall and in the stigma that are able to destroy a foreign plasmid within 1 min (Roeckel et al., 1988).
Isolated sperm, egg, and central cells, as well as zygotes, offer new possibilities for studying the cellular and molecular physiology of fertilization (Dumas and Russell, 1992; Kranz et al., 1992). The recent development of in vitro fertilization methods that do not require electrofusion (J.-E. Faure, C. Digonnet-Kerhoas, and C. Dumas, unpublished results) may provide a useful assay for elucidating the barriers that prevent polyspermy in plants and the factors that control cell-cell adhesion and fusion. Finally, access to the earliest steps of fertilization and embryogenesis makes their molecular analysis possible, an eventuality that seemed improbable only a few years ago (Goldberg et al., 1989). In this way, genes specifically expressed in sperm cells might, for example, be compared with genes specifically expressed in pollen (see Mascarenhas, 1993, this issue; McCormick, 1993, this issue). Control of cell polarity and the first division that leads to two embryo cells (the basal cell, the origin of the suspensor, and the apical cell, the origin of the embryo proper; see West and Harada, 1993, this issue) might be analyzed from cDNA libraries developed from egg cells.
ACKNOWLEDGMENTS
We thank A. Chaboud, J.-E. Faure, E. Kranz, H. Lörz, and V. Wagner for providing materials. Research support for CD. has been provided by a Centre Nationale de la Recherche Scientifique-lnstitut Nation-ale de la Recherche Agronomique grant, a PROCOPE grant, and by National Science Foundation (NSF)-Centre Nationale de la Recherche Scientifique joint program Grant No. INT-8815251. Research support for H.L.M. has been provided by U.S. Department of Agriculture Grant No. 88-37234-3876, NSF Grant No. DCB-9103658, NSF-Centre Nation-ale de la Recherche Scientifique joint program Grant No. INT-8815251, and by the Organized Research Fund of Northern Arizona University.
REFERENCES
Barnabas, B. (1985). Effect of water loss on germination ability of maize (Zea mays) pollen. Ann. Bot. 48, 861-864.
Bedinger, P. (1992). The remarkable biology of pollen. Plant Cell 4, 879-887.
Bellmann, R., and Werr, W. (1992). ZmHoxIa, the product of a novel maize homeobox gene, interacts with the Shrunken 26-bp feedback control element. EMBO J. 11, 3367-3374.
Booy, G., Krens, F.A., and Bino, R.J. (1992). Analysis of pollen tube growth in cultured maize silks. Sex. Plant Reprod. 5, 227-231.
Campenot, M.K., Zhang, G., Cutler, A.J., and Cass, D.D. (1992). Zea mays embryo sacs in culture. I. Plant regeneration from 1 day after pollination embryos. Am. J. Bot. 79, 1368-1373.
Carlson, W.R. (1988). B chromosomes as a model system for nondisjunction. In Aneuploidy, Part B, Induction and Test Systems, B. Vig and A. Sandberg, eds (New York: Alan R. Liss), pp. 199-207.
Cass, D.D., and Fabi, G.C. (1988). Structure and properties of sperm cells isolated from the pollen of Zea mays. Can. J. Bot. 66,819-825.
Chaboud, A., and Perez, R. (1992). Generative cells and male gametes: Isolation, physiology, and biochemistry. Int. Rev. Cytol. 140, 205-232.
Chaboud, A., Perez, R., Digonnet, C, and Dumas, C. (1992). Gamete recognition in angiosperms: Model and strategy for analysis. In Perspectives in Plant Cell Recognition, J. Callow and J.R. Green, eds (Cambridge: Cambridge University Press), pp. 59-77.
Chapman, G.P., Mantell, S.H., and Daniels, R.W., eds (1985). The Experimental Manipulation of Ovule Tissues. (New York: Longman).
Clark, J.K., and Sheridan, W.F. (1991). Isolation and characterization of 51 embryo-specific mutations of maize. Plant Cell 3,935-951.
Dale, B. (1991). Mechanism of fertilization: Plants to humans. In Cell to Cell Signals in Plants and Animals, V. Neuhoff and J. Friend, eds, NATO ASI Series Vol. H51 (Berlin: Springer-Verlag), pp. 83-90.
Delvallee, I., Guillaud, J., Beckert, M., and Dumas, C. (1989). Cryopreservation of immature maize embryos after freeze-hardening in the ear and in vitro. Plant Sci. 60, 129-136.
D'Halluin, K., Bonne, E., Bossut, M., De Beuckeleer, M., and Leemans, J. (1992). Transgenic maize plants by tissue electropora-tion. Plant Cell 4, 1495-1505.
Diboll, A.G. (1968). Fine structural development of the megagameto-phyte of Zea mays following fertilization. Am. J. Bot. 55, 797-806.
Diboll, A.G., and Larson, D.A. (1966). An electron microscopic study of the mature megagametophyte in Zea mays. Am. J. Bot. 53, 391-404.
Dow, D.A., and Mascarenhas, J.P. (1991a). Optimization of conditions for in situ hybridization and determination of the relative number of ribosomes in the cells of the mature embryo sac of maize. Sex. Plant Reprod. 4, 244-249.
Dow, D.A., and Mascarenhas, J.P. (1991b). Synthesis and accumulation of ribosomes in individual cells of the female gametophyte of maize during its development. Sex. Plant Reprod. 4, 250-253.
Dumas, C, and Russell, S.D. (1992). Plant reproductive biology. Trends. Int. Rev. Cytol. 140, 565-592.
Dumas, C, Knox, R.B., McConchie, C.A., and Russell, S.D. (1984). Emerging physiological concepts in fertilization. What's New Plant Physiol. 15, 168-174.
Dupuis, I. (1992). In vitro pollination: A new tool for analyzing environmental stress. Int. Rev. Cytol. 140, 391-406.
Dupuis, I., and Dumas, C. (1989). In vitro pollination as a model for studying fertilization in maize (Zea mays L.). Sex. Plant Reprod. 2, 265-269.
Gametes and Fertilization in Maize 1347
Dupuis, I., and Dumas, C. (1990a). Influence of temperature stress on in vitro fertilization and heat shock protein synthesis in maize (Zea mays L.) reproductive tissues. Plant. Physiol. 94, 665-670.
Dupuis, I., and Dumas, C. (1990b). Biochemical markers of female receptivity in maize (Zea mays L.) assessed using in vitro fertilization. Plant Sci. 70, 11-19.
Dupuis, I., and Pace, G. (1992). In vitro maturation of isolated maize microspores. In Reproductive Biology and Plant Breeding. XIII EUCARPIA Meeting, Angers, France, p. 37.
Dupuis, I., Roeckel, P., Matthys-Rochon, E., and Dumas, C. (1987). Procedure to isolate viable sperm cells from the corn (Zea mays) pollen grain. Plant Physiol. 85, 876-878.
Faure, J.-E., Mogensen, H.L., Kranz, E., Digonnet, C, and Dumas, C. (1992). Ultrastructural characterization and three-dimensional reconstruction of isolated maize (Zea mays L.) egg cell protoplasts. Protoplasma. 171, 97-103.
Faure, J.-E., Mogensen, H.L., Dumas, C, Lorz, H., and Kranz, E. (1993). Karyogamy after electrofusion of single egg and sperm cell protoplasts from maize: Cytological evidence and time course. Plant Cell 5, 747-755.
Freeling, M., and Walbot, V., eds (1993). The Maize Handbook. (New
York: Springer-Verlag). Friedman, W.E. (1992). Double fertilization in nonflowering seed plants
and its relevance to the origin of flowering plants. Int. Rev. Cytol.
140, 319-356.
Gaillard, A., Matthys-Rochon, E., and Dumas, C. (1992). Selection of microspore-derived embryonic structures in maize related to transformation potential by microinjection. Bot. Acta 105, 313-318.
Gengenbach, B.G. (1977). Genotypic influences on in vitro fertilization and kernel development of maize. Crop Sci. 17, 489-492.
Goldberg, R.B., Barker, S.J., and Perez-Grau, L. (1989). Regulation of gene expression during plant embryogenesis. Cell 56,146-160.
Gordon-Kamm, W.J., Spencer, T.M., Mangano, M.L., Adams, T.R., Daines, R.J., Start, W.G., O'Brien, J.V., Chambers, S.A., Adams, W.R., Jr., Willets, N.G., Rice, T.B., Mackey, C.J., Krueger, R.W., Kausch, A.P., and Lemaux, P.G. (1990). Transformation of maize cells and regeneration of fertile transgenic plants. Plant Cell 2, 603-618.
Guignard, L. (1899). Sur les antherozoides et la double copulation sexuelle chez les vegetaux angiospermiens. Rev. Gen. Bot. 11, 129-135.
Higgins, R.K., and Petolino, J.F. (1988). In vitro pollination and fertilization of maize: Influence of explant factors on kernel development. Plant Cell Tiss. Organ Cult. 12, 21-30.
Heslop-Harrison, J., and Heslop-Harrison, Y. (1970). Evaluation of pollen viability by enzymatically induced fluorescence, intracellular hydrolysis of fluorescein diacetate. Stain Technol. 45, 115-120.
Hough, T., Sing, M.B., Smart, I.J., and Knox, R.B. (1986). Immuno-fluorescent screening of monoclonal antibodies to surface antigens of animal and plant cells bound to polycarbonate membranes. J. Immun. Meth. 92, 103-107.
Huang, B.-Q., and Russell, S.D. (1992). Female germ unit: Organization, isolation, and function. Int. Rev. Cytol. 140, 233-296.
Jensen, W.A. (1973). Fertilization in flowering plants. Bioscience 23, 21-27.
Jensen, W.A. (1974). Reproduction in flowering plants. In Dynamic Aspects of Plant Ultrastructure, A.W. Robards, ed (New York: McGraw Hill), pp. 481-503.
Jensen, W.A., and Fisher, D.B. (1968). Cotton embryogenesis: The sperm. Protoplasma 65, 277-286.
Jensen, W.A., and Fisher, D.B. (1970). Cotton embryogenesis: The pollen tube in the stigma and style. Protoplasma 69, 215-235.
Kerhoas, C, Gay, G., and Dumas, C. (1987). A multidisciplinary approach to the study of the plasma membrane of Zea mays pollen during controlled dehydration. Planta 171, 1-10.
Koltunow, A.M., Treuttner, J., Cox, K.H., Wallroth, M., and Goldberg, R.B. (1990). Different temporal and spatial gene expression patterns occur during anther development. Plant Cell 2, 1201-1224.
Kranz, E., and Lörz, H. (1990). Micromanipulation and in vitro fertilization with single pollen grain of maize. Sex. Plant Reprod. 4, 160-169.
Kranz, E., and Lörz, H. (1991). In vitro fertilization with single isolated gametes—A new tool in overcoming incompatibility barriers. Proceedings of the International Conference on Overcoming Breeding Barriers, in Miyazaki, Japan, March 11-15, 1991, pp. 41-47.
Kranz, E., and Lörz, H. (1993). In vitro fertilization with isolated, single gametes results in zygotic embryogenesis and fertile maize plants. Plant Cell 5, 739-746.
Kranz, E., Bautor, J., and Lörz, H. (1991a). In vitro fertilization of single, isolated gametes of maize mediated by electrofusion. Sex. Plant Reprod. 4, 12-16.
Kranz, E., Bautor, J., and Lörz, H. (1991b). Electrofusion-mediated transmission of cytoplasmic organelles through the in vitro fertilization process, fusion of sperm cells with synergids and central cells, and cell reconstitution in maize. Sex. Plant Reprod. 4, 17-21.
Kranz, E., Lörz, H., Digonnet, C, and Faure, J.-E. (1992). In vitro fusion of gametes and production of zygotes. Int. Rev. Cytol. 140, 407-424.
Maheshwari, P. (1950). An Introduction to the Embryology of An-giosperms (McGraw-Hill: New York).
Mascarenhas, J.P. (1990). Gene activity during pollen development. Annu. Rev. Plant Physiol. Plant Mol. Biol. 41, 317-338.
Mascarenhas, J.P. (1993). Molecular mechanisms of pollen tube growth and differentiation. Plant Cell 5, 1303-1314.
Matthys-Rochon, E., Vergne, P., Detchepare, S., and Dumas, C. (1987). Male germ unit isolation from three tricellular pollen species: Brassica oleracea, Zea mays, and Triticum aestivum. Plant Physiol. 83, 464-466.
McConchie, C.A., Hough, T., and Knox, R.B. (1987). Ultrastructural analysis of the sperm cells of the mature pollen of maize, Zea mays. Protoplasma 139, 9-19.
McCormick, S. (1991). Molecular analysis on male gametogenesis in plants. Trends Genet. 7, 289-303.
McCormick, S. (1993). Male gametophyte development. Plant Cell 5, 1265-1275.
Meinke, D.W. (1991). Perspectives on genetic analysis of plant embryogenesis. Plant Cell 3, 857-866.
Mogensen, H.L. (1992). The male germ unit: Concept, composition
and significance. Int. Rev. Cytol. 140, 129-148. Mogensen, H.L., Wagner, V.T., and Dumas, C. (1990). Quantitative
three-dimensional ultrastructure of isolated corn (Zea mays) sperm
cells. Protoplasma 153, 136-140. Mol, R., Matthys-Rochon, E., and Dumas, C. (1993). In vitro culture
of fertilized embryo sacs of maize: Zygotes and two-celled proem-
bryos can develop into plants. Planta 189, 213-217.
1348     The Plant Cell
Nawaschin, S. (1898). Resultate einer Revision der Befruchtungss-vorgange bei Liliummartagon und Fritillaria tenella. Bull. Acad. Imp. Sci. St. Petersburg 33, 39-47.
Parredy, D.R., and Greyson, R.I. (1985). In vitro culture of immature
tassels of an inbred field variety of Zea mays cv. Oh43. Plant Cell
Tissue Organ Cult. 5, 119-128. Parredy, D.R., Greyson, R.I., and Walden, D.B. (1989). Production
of normal germinable and viable pollen from in w'fro-cultured maize
tassels. Theor. Appl. Genet. 77, 521-526. Pfahler, P.H. (1967). In vitro germination and pollen tube growth of
maize (Zea mays L.) pollen. I. Calcium and boron effects. Can. J.
Bot. 45, 839-845.
Reiser, L., and Fischer, R. L. (1993). The ovule and the embryo sac. Plant Cell 5, 1291-1301.
Rhodes, C.A., Lowe, T.S., and Ruby, T.L. (1988). Plant regeneration from protoplasts isolated from embryogenic maize cell cultures. Bio/Technology 6, 56-60.
Roeckel, P., Heizmann, P., Dubois, M., and Dumas, C. (1988). Attempts to transform Zea mays via pollen grains. Effect of pollen and stigma nuclease activities. Sex. Plant Reprod. 1, 156-163.
Roeckel, P., Dupuis, I., Matthys-Rochon, E., and Chaboud, A. (1991). Isolation of viable sperm cells from corn (Zea mays) pollen grains. In A Laboratory Guide for Cellular and Molecular Plant Biology, I. Negrutiu, and G. Gharti-Chhetri, eds (Basel: Birkhauser), pp. 88-96.
Roeckel, P., Moloney, M.M., and Drevet, J.R. (1992). Plant transformation using the sexual route. Int. Rev. Cytol. 140, 425-448.
Roman, H. (1948). Directed fertilization in maize. Proc. Natl. Acad. Sci. USA 34, 46-52.
Rusche, M.L. (1988). The male germ unit of Zea mays in the mature pollen grain. In Plant Sperm Cells as Tools for Biotechnology, H.J. Wilms and C.J. Keijzer, eds (Wageningen: Pudoc), pp. 61-67.
Rusche, M.L., and Mogensen, H.L. (1988). The male germ unit of Zea mays: Quantitative ultrastructure and three dimensional analysis. In Sexual Reproduction in Higher Plants, M. Cresti, P. Gori, and E. Pacini, eds (Berlin: Springer-Verlag), pp. 221-226.
Russell, S.D. (1979). Fine structure of megagametophyte development in Zea mays. Can. J. Bot. 57, 1093-1110.
Russell, S.D. (1985). Preferential fertilization in Plumbago: Ultrastruc-tural evidence for gamete-level recognition in an angiosperm. Proc. Natl. Acad. Sci. USA 82, 6129-6132.
Russell, S.D. (1991). Isolation and characterization of sperm cells in flowering plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 42, 189-204.
Russell, S.D. (1992) Double fertilization. Int. Rev. Cytol. 140,357-390.
Russell, S.D. (1993). The egg cell: Development and role in fertilization and early embryogenesis. Plant Cell 5, 1349-1359.
Russell, S.D., and Dumas, C, eds (1992). Sexual Reproduction in Flowering Plants. Int. Rev. Cytol. 140, 1-615.
Russell, S.D., Cresti, M., and Dumas, C. (1990). Recent progress on sperm characterization in flowering plants. Physiol. Plant. 80, 669-676.
Sheridan, W.S. (1982). Maize for Biological Research (Grand Forks, ND: University of North Dakota Press).
Sheridan, W.S. (1988). Maize developmental genetics: Genes of morphogenesis. Annu. Rev. Genet. 22, 353-385.
Sladky, Z., and Havel, L. (1976). The study for the fertilization in vitro in maize. Biol. Plant 18, 469-472.
Southworth, D. (1992). Freeze fracture of male reproductive cells. Int.
Rev. Cytol. 140, 187-204. Theunis, C.H., Pierson, E.S., and Cresti, M. (1991). Isolation of male
and female gametes in higher plants. Sex. Plant Reprod. 4,145-154. Van Lammeren, A.A.M. (1986). A comparative ultrastructural study
of the megagametophytes in two strains of Zea mays L. before and
after fertilization. Agric. Univ. Wageningen Papers 86, 1-37. Veit, B., Schmidt, R.J., Hake, S., and Yanofsky, M.F. (1993). Maize
floral development: New genes and old mutants. Plant Cell 5,
1205-1215.
Vollbrecht, E., Veit, B., Sinha, N., and Hake, S. (1991). The developmental gene Knotted-1 is a member of a maize homeobox gene family. Nature 350, 241-243.
Walbot, V. (1991). Maize mutants for the 21st century. Plant Cell 3, 851-856.
Wagner, V.T., Dumas, C, and Mogensen, H.L. (1989a). Morphometry analysis of isolated Zea mays sperm. J. Cell Sci. 93, 179-184.
Wagner, V.T., Dumas, C, and Mogensen, H.L. (1990). Quantitative three-dimensional study on the position of the female gametophyte and its constituent cells as a prerequisite for corn (Zea mays) transformation. Theor. Appl. Genet. 79, 72-76.
Wagner, V.T., Song, Y.C., Matthys-Rochon, E., and Dumas, C. (1989b). Observations on the isolated embryo sac of Zea mays L. Plant Sci. 59, 127-132.
Walker, J.C., and Zang, R. (1990). Relationship of a putative receptor protein kinase from maize to the S-locus glycoproteins of Brassica. Nature 345, 743-746.
West, M.A.L., and Harada, J.J. (1993). Embryogenesis in higher plants: An overview. Plant Cell 5, 1361-1369.
Yang, H.Y., and Zhou, C. (1989). Isolation of viable sperms from pollen of Brassica napus, Zea mays and Secale cereale. Chin. J. Bot. 1, 80-84.
Zenkteler, M. (1990). In vitro fertilization and wide hybridization in higher plants. CRC Crit. Rev. Plant Sci. 9, 267-279.
Zenkteler, M. (1992). Wide hybridization in higher plants by applying the method of test tube pollination of ovules. In Reproductive Biology and Plant Breeding, Y. Dattee, C. Dumas, and A. Gallais, eds (Berlin: Springer-Verlag), pp. 205-214.
Zhang, G., Campenot, M.K., McGann, L.E., and Cass, D.D. (1992a). Flow cytometric characteristics of sperm cells isolated from pollen of Zea mays L. Plant Physiol. 99, 54-59.
Zhang, G., Williams, CM., Campenot, M.K., McGann, L.E., and Cass, D.D. (1992b). Improvement of longevity and viability of sperm cells isolated from pollen of Zea mays L. Plant Physiol. 100,47-53.
Zhou, C. (1985). A study of fertilization events in living embryo sacs isolated from sunflower ovules. Plant Sci. 52, 147-153.