Report LightCycler® 480 Software . iiiiiiiiiii ^Roche) 02122014blaZkvantifikace Experiment Creation Date 12/2/2014 2:41:19 PM Last Modified Date 12/2/2014 4:01:22 PM Operator LMDM Owner LMDM Start Time 12/2/2014 2:52:39 PM End Time 12/2/2014 3:56:45 PM Run State Completed Software Version LCS480 1.5.1.62 Macro Macro Owner Macro Status Templates blaZ kvantifikace cviko Plate ID 03413007 Test ID Lot ID Color Comp ID Run Notes dhghrhjdrjj. u,ldr,r,.ryr.ru.;.;d Programs Program Name stage 1 Cycles 1 Analysis Mode None Target (°C) Acquisition Mode Hold (hh:mm:ss) Ramp Rate (°C/s) Acquisitions (per °C) Sec Target (°C) Step size (°C) Step Delay (cycles) 50 None 00:02:00 4.40 0 0 0 Program Name stage 2 Cycles 1 Analysis Mode None Target (°C) Acquisition Mode Hold (hh:mm:ss) Ramp Rate (°C/s) Acquisitions (per °C) Sec Target (°C) Step size (°C) Step Delay (cycles) 95 None 00:05:00 4.40 0 0 0 Program Name stage 3 Cycles 40 Analysis Mode Quantification Target (°C) Acquisition Mode Hold (hh:mm:ss) Ramp Rate (°C/s) Acquisitions (per °C) Sec Target (°C) Step size (°C) Step Delay (cycles) 95 None 00:00:10 4.40 0 0 0 60 Single 00:00:30 2.20 0 0 0 Program Name stage 4 Cycles 1 Analysis Mode Melting Curves Target (°C) Acquisition Mode Hold (hh:mm:ss) Ramp Rate (°C/s) Acquisitions (per °C) Sec Target (°C) Step size (°C) Step Delay (cycles) 95 None 00:00:15 4.40 0 0 0 60 None 00:01:00 2.20 0 0 0 95 Continuous 0.11 5 0 0 0 02122014blaZkvantifikace 12/2/2014 Page 1 of 8 Program Name cooling Cycles 1 Analysis Mode None Target Acquisition Hold Ramp Rate Acquisitions Sec Target Step size Step Delay (°C) Mode (hh:mm:ss) (°C/s) (per °C) (°C) (°C) (cycles) 40 None 00:01:00 2.20 0 0 0 Samples Sample Count Subset 96 All Samples Pos Name ID Repl. Of Sample Notes A1 PUB A1 A2 PUB A1 A3 PUB A1 A4 PUB A4 A5 PUB A4 A6 PUB A4 A7 PUB A7 A8 PUB A7 A9 PUB A7 A10 PUB A10 A11 PUB A10 A12 PUB A10 B1 PUB B1 B2 PUB B1 B3 PUB B1 B4 PUB B4 B5 PUB B4 B6 PUB B4 B7 PUB B7 B8 PUB B7 B9 PUB B7 B10 Sample 22 B11 Sample 23 B12 Sample 24 C1 629B C1 C2 629B C1 C3 629B C1 C4 629B redeno C4 C5 629B redeno C4 C6 629B redeno C4 C7 759B C7 C8 759B C7 C9 759B C7 02122014blaZkvantifikace 12/2/2014 Page 2 of 8 Samples Sample Count] 96 Subset All Samples Pos Name ID Repl. Of Sample Notes C10 759B redeno C10 C11 759B redeno C10 C12 759B redeno C10 D1 Neg_kontr D1 D2 Neg_kontr D1 D3 Neg_kontr D1 D4 Sample 40 D5 Sample 41 D6 Sample 42 D7 Sample 43 D8 Sample 44 D9 Sample 45 D10 Sample 46 D11 Sample 47 D12 Sample 48 E1 Sample 49 E2 Sample 50 E3 Sample 51 E4 Sample 52 E5 Sample 53 E6 Sample 54 E7 Sample 55 E8 Sample 56 E9 Sample 57 E10 Sample 58 E11 Sample 59 E12 Sample 60 F1 Sample 61 F2 Sample 62 F3 Sample 63 F4 Sample 64 F5 Sample 65 F6 Sample 66 F7 Sample 67 F8 Sample 68 F9 Sample 69 F10 Sample 70 F11 Sample 71 F12 Sample 72 02122014blaZkvantifikace 12/2/2014 Page 3 of 8 Samples Sample Count] 96 Subset All Samples Pos Name ID Repl. Of Sample Notes G1 Sample 73 G2 Sample 74 G3 Sample 75 G4 Sample 76 G5 Sample 77 G6 Sample 78 G7 Sample 79 G8 Sample 80 G9 Sample 81 G10 Sample 82 G11 Sample 83 G12 Sample 84 H1 Sample 85 H2 Sample 86 H3 Sample 87 H4 Sample 88 H5 Sample 89 H6 Sample 90 H7 Sample 91 H8 Sample 92 H9 Sample 93 H10 Sample 94 H11 Sample 95 H12 Sample 96 Revision History Reusion Date User Reason Abs Quant/2nd Derivative Max for All Samples (Abs Quant/2nd Derivative Max) Settings Channel 465-510 Color Compensation Off Program stage 3 Units Mode High Confidence Subset Name All Samples 02122014blaZkvantifikace 12/2/2014 Page 4 of 8 Results Inc Pos Name Type CP Concentration Standard Status □ A1 PUB Standard 1.00E2 □ A2 PUB Standard 1.00E2 □ A3 PUB Standard 1.00E2 0 A4 PUB Standard 10.57 1.16E1 1.00E1 0 A5 PUB Standard 9.69 1.86E1 1.00E1 0 A6 PUB Standard 10.34 1.32E1 1.00E1 0 A7 PUB Standard 15.33 9.10E-1 1.00E0 0 A8 PUB Standard 15.48 8.41 E-1 1.00E0 0 A9 PUB Standard 15.72 7.39E-1 1.00E0 0 A10 PUB Standard 20.16 6.90E-2 1.00E-1 0 A11 PUB Standard 19.72 8.72E-2 1.00E-1 0 A12 PUB Standard 20.23 6.65E-2 1.00E-1 0 B1 PUB Standard 24.52 6.68E-3 1.00E-2 0 B2 PUB Standard 23.56 1.12E-2 1.00E-2 0 B3 PUB Standard 23.70 1.04E-2 1.00E-2 0 B4 PUB Standard 28.22 9.23E-4 1.00E-3 0 B5 PUB Standard 27.63 1.26E-3 1.00E-3 0 B6 PUB Standard 27.17 1.62E-3 1.00E-3 0 B7 PUB Standard 31.91 1.08E-4 1.00E-4 0 B8 PUB Standard 32.03 1.00E-4 1.00E-4 0 B9 PUB Standard 32.40 7.94E-5 1.00E-4 0 B10 Sample 22 Unknown 0 B11 Sample 23 Unknown 0 B12 Sample 24 Unknown 0 C1 629B Unknown 12.50 4.14E0 0 C2 629B Unknown 13.05 3.09E0 0 C3 629B Unknown 12.61 3.91 EO 0 C4 629B_redeno Unknown 16.61 4.60E-1 0 C5 629B_redeno Unknown 16.22 5.68E-1 0 C6 629B_redeno Unknown 16.15 5.87E-1 0 C7 759B Unknown 15.46 8.48E-1 0 C8 759B Unknown 14.84 1.18E0 0 C9 759B Unknown 15.05 1.06E0 0 C10 759B_redeno Unknown 18.10 2.07E-1 0 C11 759B_redeno Unknown 17.75 2.49E-1 0 C12 759B_redeno Unknown 18.00 2.18E-1 0 D1 Neg_kontr Negate Control 02122014blaZkvantifikace 12/2/2014 Page 5 of 8 Results Inc Pos Name Type CP Concentration Standard Status 0 D2 Neg_kontr Negate Control 0 D3 Neg_kontr Negate Control 0 D4 Sample 40 Unknown 0 D5 Sample 41 Unknown 0 D6 Sample 42 Unknown 0 D7 Sample 43 Unknown 0 D8 Sample 44 Unknown 0 D9 Sample 45 Unknown 0 D10 Sample 46 Unknown 0 D11 Sample 47 Unknown 0 D12 Sample 48 Unknown 0 E1 Sample 49 Unknown 0 E2 Sample 50 Unknown 0 E3 Sample 51 Unknown 0 E4 Sample 52 Unknown 0 E5 Sample 53 Unknown 0 E6 Sample 54 Unknown 0 E7 Sample 55 Unknown 0 E8 Sample 56 Unknown 0 E9 Sample 57 Unknown 0 E10 Sample 58 Unknown 0 E11 Sample 59 Unknown 0 E12 Sample 60 Unknown 0 F1 Sample 61 Unknown 0 F2 Sample 62 Unknown 0 F3 Sample 63 Unknown 0 F4 Sample 64 Unknown 0 F5 Sample 65 Unknown 0 F6 Sample 66 Unknown 0 F7 Sample 67 Unknown 0 F8 Sample 68 Unknown 0 F9 Sample 69 Unknown 0 F10 Sample 70 Unknown 0 F11 Sample 71 Unknown 0 F12 Sample 72 Unknown 0 G1 Sample 73 Unknown 0 G2 Sample 74 Unknown 02122014blaZkvantifikace 12/2/2014 Page 6 of 8 Results Inc Pos Name Type CP Concentration Standard Status 0 G3 Sample 75 Unknown 0 G4 Sample 76 Unknown 0 G5 Sample 77 Unknown 0 G6 Sample 78 Unknown 0 G7 Sample 79 Unknown 0 G8 Sample 80 Unknown 0 G9 Sample 81 Unknown 0 G10 Sample 82 Unknown 0 G11 Sample 83 Unknown 0 G12 Sample 84 Unknown 0 H1 Sample 85 Unknown 0 H2 Sample 86 Unknown 0 H3 Sample 87 Unknown 0 H4 Sample 88 Unknown 0 H5 Sample 89 Unknown 0 H6 Sample 90 Unknown 0 H7 Sample 91 Unknown 0 H8 Sample 92 Unknown 0 H9 Sample 93 Unknown 0 H10 Sample 94 Unknown 18.67 1.53E-1 0 H11 Sample 95 Unknown 0 H12 Sample 96 Unknown Statistics Samples Mean Cp Std Cp Mean cone Std cone A4, A5, A6 10.20 0.46 1.45E1 3.69E0 A7, A8, A9 15.51 0.20 8.30E-1 8.60E-2 A10, A11, A12 20.03 0.27 7.42E-2 1.13E-2 B1, B2, B3 23.93 0.52 9.41 E-3 2.40E-3 B4, B5, B6 27.68 0.52 1.27E-3 3.47E-4 B7, B8, B9 32.11 0.26 9.58E-5 1.48E-5 C1, C2, C3 12.72 0.29 3.71 E0 5.48E-1 C4, C5, C6 16.33 0.25 5.38E-1 6.86E-2 C7, C8, C9 15.12 0.32 1.03E0 1.69E-1 C10,C11,C12 17.95 0.18 2.25E-1 2.20E-2 D1, D2, D3 02122014blaZkvantifikace 12/2/2014 Page 7 of 8 Amplification Curves 47.S4CH 42.S4CH 37.84f> 32.S4CH « 27.S4CH Lfl r 22.84CH 17.S4CH I 12.S4CH 7.84f> 2.S4CH -2.16f> Standard Curve Error: 0.0396 Efficiency: 1.707 Slope: -4.305 Ylntercept: 15.16 Link: 0.00126 — Sttf. curve Samples -2 -1.5 -1 Log Concentration ÖS Analysis Notes 02122014blaZkvantifikace 12/2/2014 Page 8 of 8