Report
LightCycler® 480 Software . iiiiiiiiiii
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02122014mecAkvantifikace Experiment
Creation Date	12/2/2014 4:40:29 PM	Last Modified Date	12/3/2014 10:52:39 AM
Operator	LMDM	Owner	LMDM
Start Time	12/2/2014 4:50:05 PM	End Time	12/2/2014 5:51:22 PM
Run State	Completed	Software Version	LCS480 1.5.1.62
Macro		Macro Owner	
Macro Status			
Templates		Plate ID	03413008
Test ID		Lot ID	
Color Comp ID			
Run Notes			
Programs
Program Name		Stagel							
Cycles		1	Analysis Mode		None				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per °C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	50	None		00:00:02	4.40		0	0	0
Program Name		Stage2							
Cycles		1	Analysis Mode		None				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per °C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:05:00	4.40		0	0	0
Program Name		Stage3							
Cycles		40	Analysis Mode		Quantification				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per °C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:00:10	4.40		0	0	0
	60	Single		00:00:30	2.20		0	0	0
Program Name		Stage4							
Cycles		1	Analysis Mode		Melting Curves				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per °C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:00:15	4.40		0	0	0
	60	None		00:01:00	2.20		0	0	0
	95	Continuous			0.11	5	0	0	0
02122014mecAk\«intifikace
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Program Name		cooling							
Cycles		1	Analysis Mode		None				
	Target	Acquisition		Hold	Ramp Rate	Acquisitions	Sec Target	Step size	Step Delay
	(°C)	Mode		(hh:mm:ss)	(°C/s)	(per °C)	(°C)	(°C)	(cycles)
	40	None		00:00:01	2.20		0	0	0
Samples
Sample Count
Subset
96
All Samples
Pos	Name	ID	Repl. Of	Sample Notes
A1	JB ehr. DNA		A1	
A2	JB ehr. DNA		A1	
A3	JB ehr. DNA		A1	
A4	JB ehr. DNA		A4	
A5	JB ehr. DNA		A4	
A6	JB ehr. DNA		A4	
A7	JB ehr. DNA		A7	
A8	JB ehr. DNA		A7	
A9	JB ehr. DNA		A7	
A10	JB ehr. DNA		A10	
A11	JB ehr. DNA		A10	
A12	JB ehr. DNA		A10	
B1	JB ehr. DNA		B1	
B2	JB ehr. DNA		B1	
B3	JB ehr. DNA		B1	
B4	JB ehr. DNA		B4	
B5	JB ehr. DNA		B4	
B6	JB ehr. DNA		B4	
B7	JB ehr. DNA		B7	
B8	JB ehr. DNA		B7	
B9	JB ehr. DNA		B7	
B10	non template		B10	
B11	non template		B10	
B12	non template		B10	
C1	07/629		C1	
C2	07/629		C1	
C3	07/629		C1	
C4	07/629redena		C4	
C5	07/629redena		C4	
C6	07/629redena		C4	
C7	07/759		C7	
C8	07/759		C7	
C9	07/759		C7	
02122014mecAk\^ntifikace
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Samples
Sample Count] 96					
Subset All Samples					
	Pos	Name	ID	Repl. Of	Sample Notes
	C10	07/759redena		C10	
	C11	07/759redena		C10	
	C12	07/759redena		C10	
	D1	Sample 37			
	D2	Sample 38			
	D3	Sample 39			
	D4	Sample 40			
	D5	Sample 41			
	D6	Sample 42			
	D7	Sample 43			
	D8	Sample 44			
	D9	Sample 45			
	D10	Sample 46			
	D11	Sample 47			
	D12	Sample 48			
	E1	Sample 49			
	E2	Sample 50			
	E3	Sample 51			
	E4	Sample 52			
	E5	Sample 53			
	E6	Sample 54			
	E7	Sample 55			
	E8	Sample 56			
	E9	Sample 57			
	E10	Sample 58			
	E11	Sample 59			
	E12	Sample 60			
	F1	Sample 61			
	F2	Sample 62			
	F3	Sample 63			
	F4	Sample 64			
	F5	Sample 65			
	F6	Sample 66			
	F7	Sample 67			
	F8	Sample 68			
	F9	Sample 69			
	F10	Sample 70			
	F11	Sample 71			
	F12	Sample 72			
02122014mecAkvantifikace 12/3/2014 Page 3 of 8
Samples
Sample Count] 96					
Subset All Samples					
	Pos	Name	ID	Repl. Of	Sample Notes
	G1	Sample 73			
	G2	Sample 74			
	G3	Sample 75			
	G4	Sample 76			
	G5	Sample 77			
	G6	Sample 78			
	G7	Sample 79			
	G8	Sample 80			
	G9	Sample 81			
	G10	Sample 82			
	G11	Sample 83			
	G12	Sample 84			
	H1	Sample 85			
	H2	Sample 86			
	H3	Sample 87			
	H4	Sample 88			
	H5	Sample 89			
	H6	Sample 90			
	H7	Sample 91			
	H8	Sample 92			
	H9	Sample 93			
	H10	Sample 94			
	H11	Sample 95			
	H12	Sample 96			
Revision History
Reusion	Date	User	Reason
			
Abs Quant/2nd Derivative Max for All Samples (Abs Quant/2nd Derivative Max) Settings
Channel	465-510		
Color Compensation	Off		
Program	Stage3	Units	
Mode	High Confidence		
Subset Name
All Samples
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve
02122014mecAkvantifikace
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Page 4 of 8
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	A1	JB chr. DNA	Standard	13.52	2.58E1	3.00E1	
0	A2	JB chr. DNA	Standard	13.71	2.29E1	3.00E1	
0	A3	JB chr. DNA	Standard	13.32	2.93E1	3.00E1	
0	A4	JB chr. DNA	Standard	16.63	3.52E0	3.00E0	
0	A5	JB chr. DNA	Standard	16.60	3.59E0	3.00E0	
□	A6	JB chr. DNA	Standard			3.00E0	
0	A7	JB chr. DNA	Standard	20.44	3.05E-1	3.00E-1	
0	A8	JB chr. DNA	Standard	31.37	2.76E-4	3.00E-1	
0	A9	JB chr. DNA	Standard	20.46	3.02E-1	3.00E-1	
0	A10	JB chr. DNA	Standard	23.89	3.33E-2	3.00E-2	
0	A11	JB chr. DNA	Standard	23.80	3.53E-2	3.00E-2	
0	A12	JB chr. DNA	Standard	24.21	2.71 E-2	3.00E-2	
0	B1	JB chr. DNA	Standard	27.87	2.60E-3	3.00E-3	
0	B2	JB chr. DNA	Standard	27.95	2.47E-3	3.00E-3	
0	B3	JB chr. DNA	Standard	28.00	2.39E-3	3.00E-3	
0	B4	JB chr. DNA	Standard	30.89	3.74E-4	3.00E-4	
0	B5	JB chr. DNA	Standard	31.13	3.20E-4	3.00E-4	
0	B6	JB chr. DNA	Standard	31.22	3.04E-4	3.00E-4	
0	B7	JB chr. DNA	Standard	31.93	1.93E-4	3.00E-5	
0	B8	JB chr. DNA	Standard	35.00	2.68E-5	3.00E-5	>
0	B9	JB chr. DNA	Standard	35.00	2.68E-5	3.00E-5	>
□	B10	non template	Negate Control				
□	B11	non template	Negate Control				
□	B12	non template	Negate Control				
0	C1	07/629	Unknown	10.14	2.27E2		E
0	C2	07/629	Unknown	11.48	9.56E1		E
0	C3	07/629	Unknown	11.36	1.04E2		E
0	C4	07/629redena	Unknown	14.96	1.02E1		
0	C5	07/629redena	Unknown	15.17	8.96E0		
0	C6	07/629redena	Unknown	15.04	9.75E0		
0	C7	07/759	Unknown	12.74	4.27E1		E
0	C8	07/759	Unknown	12.85	3.97E1		E
0	C9	07/759	Unknown	12.84	3.99E1		E
0	C10	07/759redena	Unknown	16.20	4.63E0		
0	C11	07/759redena	Unknown	16.27	4.43E0		
0	C12	07/759redena	Unknown	16.29	4.36E0		
0	D1	Sample 37	Unknown				
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve
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Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	D2	Sample 38	Unknown				
0	D3	Sample 39	Unknown				
0	D4	Sample 40	Unknown				
0	D5	Sample 41	Unknown				
0	D6	Sample 42	Unknown				
0	D7	Sample 43	Unknown				
0	D8	Sample 44	Unknown				
0	D9	Sample 45	Unknown				
0	D10	Sample 46	Unknown				
0	D11	Sample 47	Unknown				
0	D12	Sample 48	Unknown				
0	E1	Sample 49	Unknown				
0	E2	Sample 50	Unknown				
0	E3	Sample 51	Unknown				
0	E4	Sample 52	Unknown				
0	E5	Sample 53	Unknown				
0	E6	Sample 54	Unknown				
0	E7	Sample 55	Unknown				
0	E8	Sample 56	Unknown				
0	E9	Sample 57	Unknown				
0	E10	Sample 58	Unknown				
0	E11	Sample 59	Unknown				
0	E12	Sample 60	Unknown				
0	F1	Sample 61	Unknown				
0	F2	Sample 62	Unknown				
0	F3	Sample 63	Unknown				
0	F4	Sample 64	Unknown				
0	F5	Sample 65	Unknown				
0	F6	Sample 66	Unknown				
0	F7	Sample 67	Unknown				
0	F8	Sample 68	Unknown				
0	F9	Sample 69	Unknown				
0	F10	Sample 70	Unknown				
0	F11	Sample 71	Unknown				
0	F12	Sample 72	Unknown				
0	G1	Sample 73	Unknown	18.10	1.37E0		
0	G2	Sample 74	Unknown				
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve
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Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	G3	Sample 75	Unknown				
0	G4	Sample 76	Unknown				
0	G5	Sample 77	Unknown				
0	G6	Sample 78	Unknown				
0	G7	Sample 79	Unknown				
0	G8	Sample 80	Unknown				
0	G9	Sample 81	Unknown				
0	G10	Sample 82	Unknown				
0	G11	Sample 83	Unknown				
0	G12	Sample 84	Unknown				
0	H1	Sample 85	Unknown				
0	H2	Sample 86	Unknown				
0	H3	Sample 87	Unknown				
0	H4	Sample 88	Unknown				
0	H5	Sample 89	Unknown				
0	H6	Sample 90	Unknown				
0	H7	Sample 91	Unknown				
0	H8	Sample 92	Unknown				
0	H9	Sample 93	Unknown				
0	H10	Sample 94	Unknown				
0	H11	Sample 95	Unknown				
0	H12	Sample 96	Unknown				
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve
Statistics
Samples	Mean Cp	Std Cp	Mean cone	Std cone
A1, A2, A3	13.52	0.19	2.60E1	3.20E0
A4, A5	16.61	0.02	3.56E0	5.15E-2
A7, A8, A9	24.09	6.30	2.03E-1	1.75E-1
A10, A11, A12	23.97	0.22	3.19E-2	4.27E-3
B1, B2, B3	27.94	0.07	2.49E-3	1.09E-4
B4, B5, B6	31.08	0.17	3.33E-4	3.67E-5
B7, B8, B9	33.98	1.77	8.22E-5	9.58E-5
C1, C2, C3	10.99	0.74	1.42E2	7.34E1
C4, C5, C6	15.06	0.11	9.65E0	6.44E-1
C7, C8, C9	12.81	0.06	4.08E1	1.69E0
C10,C11,C12	16.25	0.05	4.47E0	1.37E-1
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Amplification Curves
Standard Curve
— Sttf. curve Samples
Error: 0.0213 Efficiency: 1.899 Slope: -3.590 Ylntercept: 18.59 Link: 0.000
-2 -1.5 -1 Log Concentration
1.5
Analysis Notes
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