Fotoinhibice fotosyntézy
fluorometrické metody stanovení
M. Barták
Produkční biologie rostlin
Metody fluorescence chlorofylu
Podzim  2012

Textové pole: http://www.esf.edu/efb/schulz/Limnology/phytoecol1.html
http://www.esf.edu/efb/schulz/Limnology/phytoecol1.html
PI2

http://www.jeffs251lab.com/category/lab4/
Photosynthesis-Curve1


Basic de-excitation mechanisms of Chl a
n
Chl a*
Photosynth.process (linear transport e- )
Thermal dissipation
Regulation between PSII and PSI
Chlorophyll fluorescence

Reversible transport
n                                      Qa
n     3(P680+ Pheo-)          1(P680+ Pheo-)
n
n
n          3 P680*                                   1 P680*
n
n3 B carotene*    P 680                 Fluorescence
n                   O2*
n3 B carotene*
n
3Chla*           Chla*
heat
heat
Singlet
Recombin.
Charge
separation
Triplet
Recomb.
Antennae q.
Photosynthetic pathway

http://www.clr.pdx.edu/projects/lakes/waldo.php
time-series-FVFm
Fv/Fm  : potential yield of photochemical processes in photosystem II
daily course of Fv/Fm indicates light-dependent adjustment
of photosynthetic processes (lower effectivity )

puddlelake
qP  = (Fm’- F) / (Fm’-Fo’)                       Fm’-= Fms
NPQ = (Fm - Fm’) / (Fm’)
qN = qE + qT + qI
qE (energy-dependent quenching)
Slow Chl fluorescence kinetics with quenching analysis

http://www.biol.s.u-tokyo.ac.jp/users/seitaipl/personal/terashima/terashima_e.html
fig5
Control – before stress treatment
Just after stress treatment
Partial recovery
After 60 min. in dark