SECOND DERIVATIVE			
			
Samples	MeanCp	STD Cp	
"C1, D1"	22.92383721	0.047875658	
"C2, D2"	25.83890216	0.27383692	
"C3, D3"	21.54948291	0.528780968	
"C4, D4"	21.46518144	0.129699175	
"C6, D6"	27.4464008	0.024057648	
"C7, D7"	23.00674242	0.010596774	
"C8, D8"	23.58475822	0.036264242	
"C9, D9"	0	0	
"C10, D10"	0	0	
"E1, F1"	14.88371791	0.064857509	
"E2, F2"	14.8880545	0	
"E3, F3"	14.85874923	0.027447604	
"E4, F4"	14.93108556	0.042899481	
			
Include	Pos	Name	Cp
True	C1	HPRT DMSO I	22.89
True	C2	HPRT TCDD 1	25.65
True	C3	HPRT DMSO II	21.92
True	C4	HPRT TCDD II	21.56
True	C5	ABC B1 DMSO	
True	C6	ABC B1 TCDD	27.43
True	C7	ABC C1 DMSO	23.01
True	C8	ABC C1 TCDD	23.61
True	D1	HPRT DMSO I	22.96
True	D2	HPRT TCDD 1	26.03
True	D3	HPRT DMSO II	21.18
True	D4	HPRT TCDD II	21.37
True	D5	ABC B1 DMSO	
True	D6	ABC B1 TCDD	27.46
True	D7	ABC C1 DMSO	23
True	D8	ABC C1 TCDD	23.56
True	E1	GAPDH DMSO I	14.84
True	E2	GAPDHT TCDD I	14.89
True	F1	GAPDH DMSO I	14.93
True	F2	GAPDHT TCDD I	
			
			
FIT POINTS			
			
Include	Pos	Name	Cp
True	C1	HPRT DMSO I	22.89
True	C2	HPRT TCDD 1	25.65
True	C3	HPRT DMSO II	21.92
True	C4	HPRT TCDD II	21.56
True	C5	ABC B1 DMSO	
True	C6	ABC B1 TCDD	27.43
True	C7	ABC C1 DMSO	23.01
True	C8	ABC C1 TCDD	23.61
True	D1	HPRT DMSO I	22.96
True	D2	HPRT TCDD 1	26.03
True	D3	HPRT DMSO II	21.18
True	D4	HPRT TCDD II	21.37
True	D5	ABC B1 DMSO	
True	D6	ABC B1 TCDD	27.46
True	D7	ABC C1 DMSO	23
True	D8	ABC C1 TCDD	23.56
True	E1	GAPDH DMSO I	15.08
True	E2	GAPDHT TCDD I	15.12
True	E3	GAPDH DMSO II	14.97
True	E4	GAPDH TCDD II	14.98
True	F1	GAPDH DMSO I	15.01
True	F2	GAPDHT TCDD I	15.11
True	F3	GAPDH DMSO II	14.94
True	F4	GAPDH TCDD II	14.9
			
Samples	MeanCp	STD Cp	
"C1, D1"	24.15796626	0.033981265	
"C2, D2"	29.38120391	1.22687794	
"C3, D3"	21.19222668	0.683264153	
"C4, D4"	21.45716699	0.233364996	
"C6, D6"	26.30047707	0.345705132	
"C7, D7"	23.49959838	0.22864545	
"C8, D8"	24.7071717	0.126839232	
"C9, D9"	0	0	
"C10, D10"	0	0	
"E1, F1"	15.042851	0.052746574	
"E2, F2"	15.11116369	0.00641953	
"E3, F3"	14.95458034	0.021749891	
"E4, F4"	14.94275025	0.05558291	
##### Sheet/List 2 #####
ABS. FIT POINTS			
			
Include	Pos	Name	Cp
True	A3	ABC B1 DMSO	26.77
True	A4	ABC B1 TCDD	27.68
True	A5	ABC C1 DMSO	25.27
True	A6	ABC C1 TCDD	25.69
True	A7	ABC G2 DMSO	27.02
True	A8	ABC G2 TCDD	27.61
True	B3	ABC B1 DMSO	27.11
True	B4	ABC B1 TCDD	27.77
True	B5	ABC C1 DMSO	25.62
True	B6	ABC C1 TCDD	25.67
True	B7	ABC G2 DMSO	27.35
True	B8	ABC G2 TCDD	27.86
			
			
Samples	MeanCp	STD Cp	
"A1, B1"	0	0	
"A2, B2"	0	0	
"A3, B3"	26.93802261	0.238182411	
"A4, B4"	27.72399197	0.062346097	
"A5, B5"	25.44446044	0.24621653	
"A6, B6"	25.6824303	0.01423861	
"A7, B7"	27.18605132	0.233230616	
"A8, B8"	27.7350624	0.175130143	
			
			
2ND DERIVATIVE			
			
Include	Pos	Name	Cp
True	A3	ABC B1 DMSO	27.34
True	A4	ABC B1 TCDD	27.85
True	A5	ABC C1 DMSO	25.18
True	A6	ABC C1 TCDD	25.07
True	A7	ABC G2 DMSO	26.12
True	A8	ABC G2 TCDD	26.63
True	B3	ABC B1 DMSO	27.29
True	B4	ABC B1 TCDD	27.55
True	B5	ABC C1 DMSO	25.22
True	B6	ABC C1 TCDD	25.23
True	B7	ABC G2 DMSO	26.22
True	B8	ABC G2 TCDD	26.39
			
Samples	MeanCp	STD Cp	
"A1, B1"	0	0	
"A2, B2"	0	0	
"A3, B3"	27.31697159	0.036702946	
"A4, B4"	27.70341095	0.213891605	
"A5, B5"	25.20357171	0.027401569	
"A6, B6"	25.14791291	0.111803602	
"A7, B7"	26.16886347	0.068273997	
"A8, B8"	26.51253239	0.16760838	
##### Sheet/List 3 #####
Cviceni qRTPCR 2016	qRT-PCR cviceni moderni metody 2016						
							
"""Melting curve"""							
							
							
							
							
							
							
							
							
							
							
							
							
							
							
							
							
							
							
							
							
							
							
							
							
							
							
							
pro vsechny geny jsme ziskali jen jeden produkt - kontrola specifity reace probehla uspesne							
VYHODNOCENI FIT POINTS							
		MEAN Cp	ST. DEV				
GAPDH DMSO	15.042851	15.042851	0.052746574				
GAPDH TCDD	15.11116369	15.11116369	0.00641953		misto HPRT jsme vzali jako referencni house-keeping		
ABC B1 DMSO		26.93802261	0.238182411		"gene GAPDH, protoze dava vice konzistentni vysledky"		
ABC B1 TCDD		27.72399197	0.062346097		neovlivnene TCDD		
ABC C1 DMSO		25.44446044	0.24621653				
ABC C1 TCDD		25.6824303	0.01423861		1. porovnej vysledky ziskane pomoci fit points a 		
ABC G2 DMSO		27.18605132	0.233230616		druhe derivace a do dalsiho vyhodnoceni vezmi 		
ABC G2 TCDD		27.7350624	0.175130143		druhou derivaci		
							
VYHODNOCENI 2nd DERIVATIVE					2. podle tabulky v protokolu vyhodnot vysledky		
GAPDH DMSO		14.88371791	0.064857509			DCp	2^-DCp
GAPDH TCDD		14.8880545	0.057896				
ABC B1 DMSO		27.31697159	0.036702946		3. porovnej vliv TCDD na expresi ABC transporteru		
ABC B1 TCDD		27.70341095	0.213891605				
ABC C1 DMSO		25.20357171	0.027401569				
ABC C1 TCDD		25.14791291	0.111803602				
ABC G2 DMSO		26.16886347	0.068273997				
ABC G2 TCDD		26.51253239	0.16760838