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LightCycler® 480 Software
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20161129tetKskupina II Experiment
Creation Date	11/29/2016 4:00:24 PM	Last Modified Date	11/29/2016 5:51:55 PM
Operator	LMDM	Owner	LMDM
Start Time	11/29/2016 4:07:46 PM	End Time	11/29/2016 5:12:27 PM
Run State	Completed	Software Version	LCS480 1.5.1.62
Macro		Macro Owner	
Macro Status			
Templates	blaZ kvantifikace cviko	Plate ID	C0384607
Test ID		Lot ID	
Color Comp ID			
Run Notes	dhghrhjdrjj. u,ldr,r,.ryr.ru.;.;d		
Programs
Program Name		stage 1							
Cycles			Analysis Mode		None				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	50	None		00:02:00	4.40		0	0	0
Program Name Cycles		stage 2							
		1	Analysis Mode		None				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:05:00	4.40		0	0	0
Program Name		stage 3							
Cycles		40	Analysis Mode | Quantification						
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:00:10	4.40		0	0	0
	60	Single		00:00:30	2.20		0	0	0
Program Name		stage 4							
Cycles		1	Analysis Mode		Melting Curves				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:00:15	4.40		0	0	0
	60	None		00:01:00	2.20		0	0	0
	95	Continuous			0.11	5	0	0	0
20161129tetKskupina II
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Page 1 of 9
Program Name		cooling							
Cycles		1	Analysis Mode		None				
	Target	Acquisition		Hold	Ramp Rate	Acquisitions	Sec Target	Step size	Step Delay
	(°C)	Mode		(hh:mm:ss)	(°C/s)	(per°C)	(°C)	(°C)	(cycles)
	40	None		00:01:00	2.20		0	0	0
Samples
Sample Count
Subset
96
All Samples
Pos	Name	ID	Repl. Of	Sample Notes
A1	pT181 DNA		A1	
A2	pT181 DNA		A1	
A3	pT181 DNA		A1	
A4	pT181 DNA		A4	
A5	pT181 DNA		A4	
A6	pT181 DNA		A4	
A7	pT181 DNA		A7	
A8	pT181 DNA		A7	
A9	pT181 DNA		A7	
A10	pT181 DNA		A10	
A11	pT181 DNA		A10	
A12	pT181 DNA		A10	
B1	pT181 DNA		B1	
B2	pT181 DNA		B1	
B3	pT181 DNA		B1	
B4	pT181 DNA		B4	
B5	pT181 DNA		B4	
B6	pT181 DNA		B4	
B7	pT181 DNA		B7	
B8	pT181 DNA		B7	
B9	pT181 DNA		B7	
B10	pT181 DNA		B10	
B11	pT181 DNA		B10	
B12	pT181 DNA		B10	
C1	10		C1	
C2	10		C1	
C3	10		C1	
C4	100		C4	
C5	100		C4	
C6	100		C4	
C7	10		C7	
C8	10		C7	
C9	10		C7	
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Samples
Sample Count
96
Subset All Samples
Pos	Name	ID	Repl. Of	Sample Notes
C10	100		C10	
C11	100		C10	
C12	100		C10	
D1	H20		D1	
D2	H20		D1	
D3	H20		D1	
D4	H20		D4	
D5	H20		D4	
D6	H20		D4	
D7	Sample 43			
D8	Sample 44			
D9	Sample 45			
D10	Sample 46			
D11	Sample 47			
D12	Sample 48			
E1	Sample 49			
E2	Sample 50			
E3	Sample 51			
E4	Sample 52			
E5	Sample 53			
E6	Sample 54			
E7	Sample 55			
E8	Sample 56			
E9	Sample 57			
E10	Sample 58			
E11	Sample 59			
E12	Sample 60			
F1	Sample 61			
F2	Sample 62			
F3	Sample 63			
F4	Sample 64			
F5	Sample 65			
F6	Sample 66			
F7	Sample 67			
F8	Sample 68			
F9	Sample 69			
F10	Sample 70			
F11	Sample 71			
F12	Sample 72			
20161129tetKskupina II
11/29/2016
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Samples
Sample Count
96
Subset All Samples
Pos	Name	ID	Repl. Of	Sample Notes
G1	Sample 73			
G2	Sample 74			
G3	Sample 75			
G4	Sample 76			
G5	Sample 77			
G6	Sample 78			
G7	Sample 79			
G8	Sample 80			
G9	Sample 81			
G10	Sample 82			
G11	Sample 83			
G12	Sample 84			
H1	Sample 85			
H2	Sample 86			
H3	Sample 87			
H4	Sample 88			
H5	Sample 89			
H6	Sample 90			
H7	Sample 91			
H8	Sample 92			
H9	Sample 93			
H10	Sample 94			
H11	Sample 95			
H12	Sample 96			
Instrument
Name	29712	Serial Number	29712
Revision History
Revision	Date	User	Reason
			
Abs Quant/2nd Derivative Max for All Samples (Abs Quant/2nd Derivative Max) Settings
Channel	465-510		
Color Compensation	Off		
Program	stage 3	Units	
Mode	High Confidence		
20161129tetKskupina II
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Subset Name All Samples
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
□	A1	pT181 DNA	Standard			1.26E5	
□	A2	pT181 DNA	Standard			1.26E5	
0	A3	pT181 DNA	Standard	6.00	1.29E5	1.26E5	
0	A4	pT181 DNA	Standard	9.13	1.32E4	1.26E4	
0	A5	pT181 DNA	Standard	9.08	1.37E4	1.26E4	
□	A6	pT181 DNA	Standard			1.26E4	
0	A7	pT181 DNA	Standard	12.59	1.07E3	1.26E3	
0	A8	pT181 DNA	Standard	12.47	1.17E3	1.26E3	
0	A9	pT181 DNA	Standard	12.58	1.08E3	1.26E3	
0	A10	pT181 DNA	Standard	15.78	1.06E2	1.26E2	
0	A11	pT181 DNA	Standard	15.96	9.26E1	1.26E2	
0	A12	pT181 DNA	Standard	15.76	1.07E2	1.26E2	
0	B1	pT181 DNA	Standard	18.43	1.54E1	1.26E1	
0	B2	pT181 DNA	Standard	18.21	1.79E1	1.26E1	
0	B3	pT181 DNA	Standard	18.21	1.79E1	1.26E1	
0	B4	pT181 DNA	Standard	22.05	1.10E0	1.26E0	
0	B5	pT181 DNA	Standard	22.02	1.13E0	1.26E0	
0	B6	pT181 DNA	Standard	21.89	1.24E0	1.26E0	
0	B7	pT181 DNA	Standard	25.23	1.25E-1	1.26E-1	
0	B8	pT181 DNA	Standard	25.14	1.33E-1	1.26E-1	
0	B9	pT181 DNA	Standard	25.01	1.45E-1	1.26E-1	
0	B10	pT181 DNA	Standard	29.15	1.25E-2	1.26E-2	
0	B11	pT181 DNA	Standard	29.18	1.23E-2	1.26E-2	
0	B12	pT181 DNA	Standard	29.03	1.33E-2	1.26E-2	
0	C1	10	Unknown				
0	C2	10	Unknown				
0	C3	10	Unknown	8.23	2.54E4		
0	C4	100	Unknown	11.77	1.94E3		
0	C5	100	Unknown	11.01	3.39E3		
0	C6	100	Unknown	11.88	1.80E3		
0	C7	10	Unknown				
0	C8	10	Unknown				
0	C9	10	Unknown	33.80	1.37E-3		E
0	C10	100	Unknown				
0	C11	100	Unknown				
E - Extrapolated concentration in standard curve
20161129tetKskupina II
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Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	C12	100	Unknown				
0	D1	H20	Negative Control	9.15	1.31E4		
0	D2	H20	Negative Control	10.28	5.75E3		
0	D3	H20	Negative Control	9.26	1.21E4		
0	D4	H20	Unknown	12.54	1.11E3		
0	D5	H20	Unknown	12.55	1.10E3		
0	D6	H20	Unknown	12.70	9.87E2		
0	D7	Sample 43	Unknown				
0	D8	Sample 44	Unknown				
0	D9	Sample 45	Unknown				
0	D10	Sample 46	Unknown				
0	D11	Sample 47	Unknown				
0	D12	Sample 48	Unknown				
0	E1	Sample 49	Unknown				
0	E2	Sample 50	Unknown				
0	E3	Sample 51	Unknown				
0	E4	Sample 52	Unknown				
0	E5	Sample 53	Unknown				
0	E6	Sample 54	Unknown				
0	E7	Sample 55	Unknown				
0	E8	Sample 56	Unknown				
0	E9	Sample 57	Unknown				
0	E10	Sample 58	Unknown				
0	E11	Sample 59	Unknown				
0	E12	Sample 60	Unknown				
0	F1	Sample 61	Unknown				
0	F2	Sample 62	Unknown				
0	F3	Sample 63	Unknown				
0	F4	Sample 64	Unknown				
0	F5	Sample 65	Unknown				
0	F6	Sample 66	Unknown				
0	F7	Sample 67	Unknown				
0	F8	Sample 68	Unknown				
0	F9	Sample 69	Unknown				
0	F10	Sample 70	Unknown				
0	F11	Sample 71	Unknown				
0	F12	Sample 72	Unknown				
E - Extrapolated concentration in standard curve
20161129tetKskupina II
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Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	G1	Sample 73	Unknown				
0	G2	Sample 74	Unknown				
0	G3	Sample 75	Unknown				
0	G4	Sample 76	Unknown				
0	G5	Sample 77	Unknown				
0	G6	Sample 78	Unknown				
0	G7	Sample 79	Unknown				
0	G8	Sample 80	Unknown				
0	G9	Sample 81	Unknown				
0	G10	Sample 82	Unknown				
0	G11	Sample 83	Unknown				
0	G12	Sample 84	Unknown				
0	H1	Sample 85	Unknown				
0	H2	Sample 86	Unknown				
0	H3	Sample 87	Unknown				
0	H4	Sample 88	Unknown				
0	H5	Sample 89	Unknown				
0	H6	Sample 90	Unknown				
0	H7	Sample 91	Unknown				
0	H8	Sample 92	Unknown				
0	H9	Sample 93	Unknown				
0	H10	Sample 94	Unknown				
0	H11	Sample 95	Unknown				
0	H12	Sample 96	Unknown				
E - Extrapolated concentration in standard curve
Statistics
Samples	Mean Cp	Std Cp	Mean cone	Std cone
A4, A5	9.11	0.04	1.35E4	3.50E2
A7, A8, A9	12.55	0.07	1.11E3	5.72E1
A10, A11, A12	15.83	0.11	1.02E2	7.86E0
B1, B2, B3	18.29	0.12	1.71E1	1.49E0
B4, B5, B6	21.99	0.08	1.16E0	7.22E-2
B7, B8, B9	25.13	0.11	1.34E-1	9.69E-3
B10, B11, B12	29.12	0.08	1.27E-2	5.52E-4
C1, C2, C3	8.23		2.54E4	
C4, C5, C6	11.55	0.47	2.37E3	8.78E2
C7, C8, C9	33.80		1.37E-3	
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Statistics
Samples	Mean Cp	Std Cp	Mean cone	Std cone
C10, C11, C12				
D1, D2, D3	9.56	0.62	1.03E4	3.97E3
D4, D5, D6	12.60	0.09	1.07E3	6.91E1
Amplification Curves
Standard Curve
Error: 0.0243		
Efficiency: 2.069		2&.
Slope: -3.167		2£
Ylntercept: 22.18		24-i
Link: 1.129		22
	* LL.	2CH
	=	iai
		
	£ u	
		
		1 CH
		
		G
Std. curve Samples
1 1.5 2 2.5 Log Concentration
3.5
20161129tetKskupina II
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Analysis Notes
20161129tetKskupina II 11/29/2016 Page 9 of 9