Version: 1 Revision Date: 2012-11-30
Product Data Sheet
Brilliant Violet 421™ Mouse IgG2a, κ Isotype Ctrl
Catalog # / Size: 400259 / 25 tests
400260 / 100 tests
Clone: MOPC-173
Isotype: Mouse IgG2a, κ
Preparation: The immunoglobulin was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal
conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Storage: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to
light. Do not freeze.
Applications:
Applications: FC, ICFC - Quality tested
Recommended Usage: Each lot of this mouse IgG2a, κ isotype control antibody is quality control tested by immunofluorescent staining with
flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your
primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended
for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Application Notes: The MOPC-173 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western
blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The
LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays
(Cat. No. 400224) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™
purified antibody (Cat. No. 400264) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin
<0.01 EU/µg).
Application References: 1. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
2. Burman AC, et al. 2007. Blood 110:1064.
3. Goo SY, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M701876200.
4. Podolin PL, et al. 2008. J. Immunol. 180:7989. PubMed
Description: The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is Mouse IgG2a, κ. This
antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat
and human tissues.
Related Products:Product Clone Application
Cell Staining Buffer FC, ICC, ICFC
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