Melting curves						
								
								
								
								
								
								
								
								
								
								
								
								
								
								
								
								
								
								
								
								
								
Ct pomocí druhé derivace								
		průměr	HPRT	DCt	2^-DCt			
ABC B1 DMSO	31.05	31.14	23.415	7.725	0.0047265			
ABC B1 DMSO	31.23							
ABC B1 TCDD	30.48	30.13	20	10.13	0.0008924			
ABC B1 TCDD	29.78							
ABC C1 DMSO	21.9	21.545	23.415	-1.87	3.6553258			
ABC C1 DMSO	21.19							
ABC C1 TCDD	32	32	20	12	0.0002441			
ABC C1 TCDD	32							
ABC G2 DMSO	20.42	20.215	23.415	-3.2	9.1895868			
ABC G2 DMSO	20.01							
ABC G2 TCDD	19.56	19.585	20	-0.415	1.3332987			
ABC G2 TCDD	19.61							
HPRT DMSO	23.6	23.415						
HPRT DMSO	23.23							
HPRT TCDD	19.89	20						
HPRT TCDD	20.11							
								
								
								
								
##### Sheet/List 2 #####
25.10.	2nd						fit						
Samples	MeanCp	STD Cp	Mean conc	STD conc			Samples	MeanCp	STD Cp	Mean conc	STD conc		
"A1, A10, A11, A12, A2, A3, A4, A5, A6, A7, A8, A9, B10, B11, B12, B3, B4, B5, B6, B7, B8, B9, C10, C11, C12, C3, C4, C5, C6, C7, C8, C9, D10, D11, D12, D3, D4, D5, D6, D7, D8, D9, E10, E11, E12, E3, E4, E5, E6, E7, E8, E9, F10, F11, F12, F3, F4, F5, F6, F7, F8, F9, G10, G11, G12, G3, G4, G5, G6, G7, G8, G9, H10, H11, H12, H3, H4, H5, H6, H7, H8, H9"	31.13743767	0.124969914	0	0			"A1, A10, A11, A12, A2, A3, A4, A5, A6, A7, A8, A9, B10, B11, B12, B3, B4, B5, B6, B7, B8, B9, C10, C11, C12, C3, C4, C5, C6, C7, C8, C9, D10, D11, D12, D3, D4, D5, D6, D7, D8, D9, E10, E11, E12, E3, E4, E5, E6, E7, E8, E9, F10, F11, F12, F3, F4, F5, F6, F7, F8, F9, G10, G11, G12, G3, G4, G5, G6, G7, G8, G9, H10, H11, H12, H3, H4, H5, H6, H7, H8, H9"	32.45416851	0.923514464	0	0		
"B1, B2"	30.13063859	0.496106915	0	0			"B1, B2"	31.01900325	0	0	0		
"C1, C2"	21.54688607	0.502657316	0	0			"C1, C2"	23.35375286	1.149032264	0	0		
"D1, D2"	32	0	0	0			"D1, D2"	33.55503275	1.106496713	0	0		
"E1, E2"	20.21395095	0.288863981	0	0			"E1, E2"	22.88434399	0.608317546	0	0		
"F1, F2"	19.58477117	0.030025318	0	0			"F1, F2"	20.51146059	0.200522715	0	0		
"G1, G2"	23.41614533	0.260689725	0	0			"G1, G2"	24.89905458	0.748023103	0	0		
"H1, H2"	20.00056331	0.157682749	0	0			"H1, H2"	23.31544661	1.790574714	0	0		
													
													
Include	Pos	Name	Cp				Include	Pos	Name	Cp	Concentration	Standard	Status
True	A1	ABC B1 DMSO	31.05				True	A1	ABC B1 DMSO	32.77		0	
True	A2	ABC B1 DMSO	31.23				True	A2	ABC B1 DMSO	31.41		0	
True	B1	ABC B1 TCDD	30.48				True	B1	ABC B1 TCDD	31.02		0	
True	B2	ABC B1 TCDD	29.78				True	B2	ABC B1 TCDD			0	
True	C1	ABC C1 DMSO	21.9				True	C1	ABC C1 DMSO	22.54		0	
True	C2	ABC C1 DMSO	21.19				True	C2	ABC C1 DMSO	24.17		0	
True	D1	ABC C1 TCDD	32				True	D1	ABC C1 TCDD	32.77		0	
True	D2	ABC C1 TCDD	32				True	D2	ABC C1 TCDD	34.34		0	
True	E1	ABC G2 DMSO	20.42				True	E1	ABC G2 DMSO	23.31		0	
True	E2	ABC G2 DMSO	20.01				True	E2	ABC G2 DMSO	22.45		0	
True	F1	ABC G2 TCDD	19.56				True	F1	ABC G2 TCDD	20.65		0	
True	F2	ABC G2 TCDD	19.61				True	F2	ABC G2 TCDD	20.37		0	
True	G1	HPRT DMSO	23.6				True	G1	HPRT DMSO	25.43		0	
True	G2	HPRT DMSO	23.23				True	G2	HPRT DMSO	24.37		0	
True	H1	HPRT TCDD	19.89				True	H1	HPRT TCDD	24.58		0	
True	H2	HPRT TCDD	20.11				True	H2	HPRT TCDD	22.05		0